Search results for "Microfluorimetry"

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From the Golgi-Cajal mapping to the transmitter-based characterization of the neuronal networks leading to two modes of brain communication: Wiring a…

2007

After Golgi-Cajal mapped neural circuits, the discovery and mapping of the central monoamine neurons opened up for a new understanding of interneuronal communication by indicating that another form of communication exists. For instance, it was found that dopamine may be released as a prolactin inhibitory factor from the median eminence, indicating an alternative mode of dopamine communication in the brain. Subsequently, the analysis of the locus coeruleus noradrenaline neurons demonstrated a novel type of lower brainstem neuron that monosynaptically and globally innervated the entire CNS. Furthermore, the ascending raphe serotonin neuron systems were found to globally innervate the forebrai…

DopamineTortuosityBrain functionWiring transmissionSynaptic TransmissionDiffusionDual probe microdialysisMicrofluorimetrychemistry.chemical_compoundCatecholaminesPressure gradientsVolume transmissionHistofluorescenceLocus coeruleusExtracellular spaceNeurological and mental disordersNeurotransmitterNeuronsNeurotransmitter AgentsGeneral NeuroscienceBrain5-HydroxytryptamineAmygdalamedicine.anatomical_structure5-Hydroxytryptamine; Amygdala; Brain function; Brain uncoupling protein-2; Catecholamines; CA turnover; Clearance; Diffusion; Dopamine; Dorsal raphe; Dual probe microdialysis; Extracellular space; Extrasynaptic receptors; Histofluorescence; Local circuits; Locus coeruleus; Mapping of monoamine neurons; Microdensitometry; Microfluorimetry; Neurological and mental disorders; Noradrenaline; Nucleus accumbens; Pressure gradients; Receptor mosaics; Receptor–receptor interactions; Substantia nigra; Thermal gradients; Tortuosity; Transmitter–receptor mismatches; Volume fraction; Volume transmission; Wiring transmissionClearanceNucleus accumbensCA turnoverLocal circuitsReceptor–receptor interactionsSilver StainingMapping of monoamine neuronsModels NeurologicalNeurotransmissionBiologySerotonergicSubstantia nigramedicineBiological neural networkAnimalsHumansThermal gradientsTransmitter–receptor mismatchesVolume fractionExtrasynaptic receptorsMonoamine neurotransmitterchemistryReceptor mosaicsForebrainNoradrenalineLocus coeruleusBrain uncoupling protein-2Neurology (clinical)NeuronNerve NetMicrodensitometry5-Hydroxytryptamine Amygdala Brain function Brain uncoupling protein-2 Catecholamines CA turnover Clearance DiffusionNeuroscienceDorsal raphe
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Acetylsalicylic acid reduces heat responses in rat nociceptive primary sensory neurons – evidence for a new mechanism of action

2002

Acetylsalicylic acid (ASA) is thought to exert its peripheral analgesic effects via inhibition of cyclooxygenase. We now studied the effects of ASA on heat responses in primary nociceptive neurons by whole-cell patch-clamp and calcium microfluorimetry experiments. Heat-evoked inward currents in acutely dissociated rat dorsal root ganglion neurons were significantly reduced by ASA in a dose-dependent and reversible manner (IC(50) 375 nM, Hill slope -2.2, maximum effect 55%). Heat-evoked calcium transients (measured with FURA-2) were reversibly reduced by 53+/-14% (P0.05) by co-application of 1 microM ASA. The low IC(50) value, the rapid occurrence, and the reversibility of the observed effec…

MaleHot TemperaturePatch-Clamp TechniquesPainchemistry.chemical_elementCalciumPharmacologyIon ChannelsMembrane PotentialsRats Sprague-Dawleychemistry.chemical_compoundDorsal root ganglionGanglia SpinalmedicineAnimalsCyclooxygenase InhibitorsThermosensingCalcium SignalingNeurons AfferentPatch clampCells CulturedAspirinDose-Response Relationship DrugGeneral NeuroscienceNociceptorsMicrofluorimetryElectric StimulationSensory neuronRatsmedicine.anatomical_structurechemistryMechanism of actionBiochemistryCapsaicinNociceptorCalciumCapsaicinmedicine.symptomSignal TransductionNeuroscience Letters
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