Search results for "Micropropagation"

Ex vitro mycorrhization of vitro-derived plantlets of 'Carrizo' citrange [C. Sinensis (L.) Osb. × P. Trifoliata (L.) Raf.]

2015

In this study, vitro-derived plantlets of 'Carrizo' citrange were inoculated with Glomus intraradices during the acclimatization phase, from in vitro to in vivo. Plantlets were grown in presence (+M) and in absence of inoculum (-M) and in addition, in sterile and non-sterile substrate. After three months of culture, some vegetative parameters and the level of infection, varied greatly in the different theses. In particular, the highest formation of new branched roots has been observed in inoculated plantlets cultivated in sterile substrate. Good results were observed also in non-inoculated plantlets cultivated in non-sterile substrate, in presence of endemic inoculum. The same trend was obs…

The effects of benzyladenine and meta-Topolin on in vitro sprouting and regrowth after encapsulation of C35 citrange [Citrus sinensis (L.) Osb. × Pon…

2016

C35, a hybrid of ‘Ruby Blood’ sweet orange and trifoliate orange, is a promising Citrus rootstock, mainly due to its resistance to Citrus Tristeza Virus (CTV), one of the most serious pathology affecting citriculture. The use of resistant rootstocks, such as citranges, is an efficient tool to fight this plant disease. This implies a continuous increasing demand of resistant rootstocks, in order to carry out the turnover of sour orange, still the most used Citrus rootstock in Italy. Micropropagation, can provide innovative solutions to obtain, in a short time and in a small space, a number of plants higher than traditional propagation. Micropropagation efficiency is also affected by the cult…

Citrus biotechnology

2020

Agricultural crops that can better withstand the changing climatic and pathogen landscape have been produced through natural selection throughout the millennia and, in recent years, through the process of human-assisted plant breeding and selection. However, a lack of genetic diversity in many commercially cultivated crops (due to monoculture) has made them more vulnerable to biotic and abiotic stresses (Esquinas-Alcazar, 2005; Keneni et al., 2012). Citrus, belonging to the Rutaceae family, is one of the most important commercial woody fruit crops in the tropical and subtropical areas of the world. The origin of citrus is traced back to parts of tropical and subtropical Southeast Asia (Wu e…

In vitro establishment and culture of two Sicilian cultivars of Olea europaea L

2012

Sicily is among the Italian regions with the largest cultivation area of olive (Olea europaea L.). The applications of in vitro tissue culture, as practical tool of propagation and germplasm storage, were not sufficiently exploited in this species, because of the lack of efficient in vitro establishment protocols and culture for the majority of the cultivars. In fact, it is well known that the success of in vitro culture is strongly dependent on the species and, within the species, on the cultivars. This study reports the in vitro establishment of two Sicilian olive cultivars, grown in greenhouse. Both genotypes showed a percentage of in vitro sprouting higher than that one previously obtai…

USE OF IN VITRO TISSUE CULTURE IN PROPAGATION AND GENETIC IMPROVEMENT OF FRUIT TREES

2020

La coltura in vitro, applicata alla propagazione e miglioramento genetico della biodiversità vegetale, può rappresentare uno strumento efficace per affrontare i problemi attuali come i cambiamenti climatici, le nuove esigenze dei consumatori e indirettamente lo sviluppo delle aree rurali. Inoltre, può assumere un ruolo strategico nel miglioramento genetico e nella propagazione delle cultivar al fine di ottenere genotipi resistenti, con frutti migliori dal punto di vista organolettico e piante capaci di adattarsi ai cambiamenti climatici. Il miglioramento genetico attraverso i metodi convenzionale è limitato da molti fattori infatti, gli alberi da frutto sono caratterizzati da un lungo perio…

In vitro propagation of Lithodora rosmarinifolia (Ten.) Johnst., a rare endemic Sicilian shrub with potential as ornamental plant.

2007

Lithodora rosmarinifolia is a rare shrub endemic to the Mediterranean island of Sicily and its minor islands. Propagation of Lithodora rosmarinifolia by standard methods is difficult due to erratic seed production and low percentage of rooted cuttings. As the plant is suited for domestication, the possibility of establishing an efficient in vitro technique for propagation and conservation of this threatened species was investigated. Nodal segments from shoot tips were used as primary explants to establish in vitro culture. A 2 X 5 factorial experiment with five concentrations (0 to 17.74 mM) of N6-benzyladenine (BA) and two concentrations (0 and 2.46 mM) of indole-3-butyric acid (IBA) was u…

Synthetic seed production of Hibiscus rosa-sinensis (L.)

2010

Endangered taxa of the Sicilian flora and conservation perspectives

2011

Perspectives of the encapsulation technology in the nursery activity of Citrus

2007

The genetic and phytosanitary certification has to guarantee the quality of the propagation material also in Citrus. Traditional methods can be assisted by the techniques of in vitro culture, which are fundamental for the mass production of high quality plants. The encapsulation technology represents an innovative tool to combine the advantages of micropropagation with handling ease, storability, reduced size of the propagules, mechanization potentiality, transportability of the gamic seeds. In this work somatic embryos obtained from in vitro anther culture of the cultivar Mandarino Tardivo di Ciaculli (Citrus reticulata Blanco) were encapsulated in a sodium alginate matrix and synthetic se…

Elimination of in vitro bacterial contaminants in shoot cultures of ‘MRS 2/5’ plum hybrid by the use of Melia azedarach extracts

2008

The antimicrobial activity of leaf and callus extracts of Melia azedarach was tested on in vitro shoot cultures of the peach rootstoch 'MRS 2/5' (Prunus cerasifera × Prunus spinosa) that were heavily contaminated with Sphingomonas paucimobilis (Sp) and Bacillus circulans (Bc). The extracts were filter-sterilised and added at 0%, 1%, 5%, 10% and 20% to a modified Murashige and Skoog proliferation medium previously autoclave-sterilised. Up to about 17% shoots died with 10-20% extract, except for Sp-contaminated shoots, whose survival was reduced to 50% after treatment with 20% extract. No shoots died with 1% to 5% supplement. The undiluted leaf extract showed bactericidal activity on plated S…