Search results for "Microsatellite"
showing 10 items of 425 documents
Microsatellite marker-based genetic variability in Spanish rice cultivars and landraces
2010
The genetic relationships among japonica rice cultivars and landraces were studied. Most of them are of Spanish origin, and were developed and cultivated for varying time periods over more than a century. To uncover genetic diversity within each cultivar, we analysed 30 plants per cultivar or accession using 10 fluorescently labelled primer pairs for SSR markers. Six cultivars were included in the study, with accessions from four different regions of Spain of the traditional cultivar Bomba. A total of 37 alleles were detected with a mean of 3.7 alleles per locus. Polymorphism information content (PIC) ranged from 0 to 0.78 with an average of 0.51 per locus. Genetic diversity for cvs. Albufe…
Evaluation of morphological and genetic diversity of loquat accessions grown in sicily
2015
In this study 20 accessions of loquat (Eriobotrya japonica Lindl.) that originated from Sicily and other countries were characterized phenotypically and genotyped using seven microsatellite markers. The microsatellites clearly distinguished 14 accessions; three pairs of local accessions were not differentiated, and three accessions from abroad can be considered bud sport mutations of the main Spanish cultivar âAlgerieâ. The mean alleles per locus was four, ranging from two with the marker EJ075 and five with EJ012 and EJ271; the mean Polymorphic Information Content was 0.53; the average expected and observed heterozygosities resulted 0.62 and 0.59, respectively. These values revealed a …
SSR-based DNA fingerprints reveal the genetic diversity of Sicilian olive (Olea europaeaL.) germplasm
2005
Twelve published simple sequence repeat (SSR; microsatellite) markers, belonging to the ssrOeUA-DCA, GAPU and UDO series, were tested in a panel of 46 accessions of olive germplasm belonging to 30 unique cultivars collected in seven Provinces of Sicily. Four well-known reference olive cultivars were also added. The analysis was carried out on an automatic capillary sequencer using fluorescent dyes, and fragment sizes were determined using internal standards. The results allowed us to rank the SSRs assayed according to their information content and reproducibility. Up to 115 alleles were identified (119, if those unique to sport mutations were included), the frequency of which allowed geneti…
Molecular and morphological diversity of on-farm hazelnut (Corylus avellana L.) landraces from southern Europe and their role in the origin and diffu…
2013
Hazelnut (Corylus avellana L.) is a traditional nut crop in southern Europe. Germplasm exploration conducted on-farm in five countries (Portugal, Spain, Italy, Slovenia, and Greece) identified 77 landraces. The present work describes phenotypic variation in nut and husk traits and investigates genetic relationships using ten simple sequence repeat (SSR) markers among these landraces, 57 well-known references cultivars, and 19 wild accessions. Among the 77 landraces, 42 had unique fingerprints while 35 showed a SSR profile identical to a known cultivar. Among the 42 unique landraces, morphological observations revealed high phenotypic diversity, and some had characteristics appreciated by th…
Centennial olive trees as a reservoir of genetic diversity
2011
†Background and Aims Genetic characterization and phylogenetic analysis of the oldest trees could be a powerful tool both for germplasm collection and for understanding the earliest origins of clonally propagated fruit crops. The olive tree (Olea europaea L.) is a suitable model to study the origin of cultivars due to its long lifespan, resulting in the existence of both centennial and millennial trees across the Mediterranean Basin. †Methods The genetic identity and diversity as well as the phylogenetic relationships among the oldest wild and cultivated olives of southern Spain were evaluated by analysing simple sequence repeat markers. Samples from both the canopy and the roots of each tr…
Genetic variation of an Italian long shelf-life tomato (Solanum lycopersicon L.) collection by using SSR and morphological fruit traits
2014
The recovery of ancient germplasm in tomato (Solanum lycopersicon L.) has become necessary to limit the wide genetic erosion caused by the employment of modern cultivars. Among germplasm collections, long shelf-life landraces could represent an important source of biodiversity. The present study provides a first set of molecular and phenotypic data on long shelf-life (so called "da serbo" in southern Italy) tomato collection, mainly originated from Sicily together with some landraces from Campania and Apulia. The analysis of fruit traits showed a low intra-varietal variation, while exhibiting a quite higher inter-varietal variability. Overall, the cultivars have been classified in six fruit…
Assessment of genetic diversity of Latvian and Swedish sweet cherry (Prunus avium L.) genetic resources collections by using SSR (microsatellite) mar…
2009
Abstract Three previously described highly polymorphic SSR (microsatellite) primer pairs were tested on 126 sweet cherry ( Prunus avium L.) accessions to adapt a fast, reliable method for preliminary screening of sweet cherry germplasm collections and to compare two sweet cherry germplasm collections: at the Latvia State Institute of Fruit-Growing, Dobele (LIFG-Dobele) and at the Division of Horticultural Genetics and Plant Breeding at Balsgard, Department of Crop Sciences, Swedish University of Agricultural Sciences (SLU-Balsgard). The SSR loci were highly polymorphic with 4–10 different alleles and 5–18 genotypes. Heterozygosity values ranged from 0.431 to 0.809, gene diversity (PIC) valu…
Characterization of five sour orange clones through molecular markers and leaf essential oils analysis.
2006
Five clones of sour orange (Citrus aurantium L.) showing significant morphological differences were selected from our germplasm collection and characterized both by genetic and leaf volatiles analysis. The genetic studies were undertaken by the use of molecular markers developed by PCR-based techniques (ISSR and RAPD), while the leaf essential oil patterns were obtained by chromatographic and mass spectrometric determination. Data obtained suggest that reasonably similar information can be achieved from the two techniques, supporting each other in characterization studies.
Spatial genetic structure reveals migration directionality in Mediterranean Ruppia spiralis (Western Sicily)
2022
Mediterranean salinas, originally built for salt production, function as alternative wetlands. A variety of accompanying lagoon, ditch, and marsh systems are suitable habitats for salt-tolerant submerged macrophytes and often characterized by monospecific beds of Ruppia. Traditionally, birds are considered the main dispersal vector of submerged macrophytes. However, Ruppia spiralis habitats are under marine influence and therefore interference of coastal currents in their connectivity might be expected. In this study, we aim to infer connectivity and spatial patterns from population genetic structures. Using nuclear microsatellite loci, the nuclear ribosomal cistron and chloroplast sequence…
Commercial sharks under scrutiny: Baseline genetic distinctiveness supports structured populations of small-spotted catsharks in the Mediterranean Sea
2023
The present study, based on microsatellite markers, describes a population genetic analysis of the small-spotted catshark Scyliorhinus canicula (Linnaeus, 1758), representing one of the most abundant and commonly caught cartilaginous fishes in the Mediterranean Sea and adjacent areas. The analyses were performed to unravel the genetic features (variability, connectivity, sex-biased dispersal) of their relative geographic populations, both at the small (around the coast of Sardinia, Western Mediterranean Sea) and at a larger spatial scale (pan-Mediterranean level and between the Atlantic Ocean and the Mediterranean Sea). Individual clustering, multivariate and variance analyses rejected the …