Search results for "Microscopy"
showing 10 items of 3390 documents
Optical-sectioning microscopy by patterned illumination
2010
We propose a very simple method for the flexible production of 1D structured illumination for high resolution 3D microscopy. Specifically, we propose the insertion of a Fresnel biprism after a monochromatic point source for producing a pair of twin, fully coherent, virtual point sources. The resulting interference fringes are projected into the 3D sample and, by simply varying the distance between the biprism and the point source, one can tune the period of the fringes, while keeping their contrast, in a very versatile and efficient way.
Optical properties of the low-molecular amorphous azochromophores and their application in holography
2015
The films based on the low-molecular amorphous azochromophore 2-(3-(4-((4- (Ethyl(2-(trityloxy)ethyl)amino)phenyl)diazenyl)styryl)-5,5-dimethylcyclohex-2-enylidene) malononitrile (IWK-2M) were prepared. The optical properties of the material, such as transmittance and reflection spectra of the film, sensitivity to polarization holographic recording by two wavelengths (405 and 532 nm) were studied. The direct relief formation during the polarization holographic recording was explored, relief depth dependence on exposure and record beam intensity was investigated. The holographic matrix on this material base was produced without chemical etching process; the replication of holographic image w…
Line shape measurements of rubidium 5S-7S two-photon transition
2014
We report the use of a digital lock to measure the line profile and center frequency of rubidium 5S-7S two-photon transitions with a cw laser referenced to an optical frequency comb. The narrow, two-photon transition, 5S-7S (760 nm), insensitive to first-order in a magnetic field, is a promising candidate for frequency reference.
Anomalous behaviour of periodic domain structure in Gd-doped LiNbO3single crystals
2007
Atomic force microscopy studies of etching patterns, stability of regular domain structure, and anomalies of electrical characteristics in the 300 - 385 K range of a series of Gddoped lithium niobate single crystals grown under equal conditions are reported.
Single-exposure super-resolved interferometric microscopy by RGB multiplexing in lensless configuration
2016
Abstract Single-Exposure Super-Resolved Interferometric Microscopy (SESRIM) reports on a way to achieve one-dimensional (1-D) superresolved imaging in digital holographic microscopy (DHM) by a single illumination shot and digital recording. SESRIM provides color-coded angular multiplexing of the accessible sample׳s range of spatial frequencies and it allows their recording in a single CCD (color or monochrome) snapshot by adding 3 RGB coherent reference beams at the output plane. In this manuscript, we extend the applicability of SESRIM to the field of digital in-line holographic microscopy (DIHM), that is, working without lenses. As consequence of the in-line configuration, an additional r…
Raman Spectroscopic Signatures of Echovirus 1 Uncoating
2014
ABSTRACT In recent decades, Raman spectroscopy has entered the biological and medical fields. It enables nondestructive analysis of structural details at the molecular level and has been used to study viruses and their constituents. Here, we used Raman spectroscopy to study echovirus 1 (EV1), a small, nonenveloped human pathogen, in two different uncoating states induced by heat treatments. Raman signals of capsid proteins and RNA genome were observed from the intact virus, the uncoating intermediate, and disrupted virions. Transmission electron microscopy data revealed general structural changes between the studied particles. Compared to spectral characteristics of proteins in the intact v…
Bactericidal Effect of the Er:YAG Laser on Dental Implant Surfaces: An In Vitro Study
2002
The aim of the in vitro study was to examine the bactericidal effect of an Er:YAG laser on common dental implant surfaces.Seventy-two titanium platelets with 3 different surfaces--sandblasted and acid-etched (SA), titanium plasma-sprayed (TPS), and hydroxyapatite-coated (HA)--were incubated with a suspension of Streptococcus sanguinis (ATCC 10556). Irradiation at pulse energies of 60 and 120 mJ and a frequency of 10 pps was performed on a computer-controlled XY translation stage. After laser treatment the specimens were sonicated and the bacterial growth examined by counting colony forming units on blood agar plates. Temperature elevations during irradiation were investigated using K-type t…
Transcription of heat shock gene loci versus non-heat shock loci in Chironomus polytene chromosomes: evidence for heat-induced formation of novel put…
1995
The heat shock response of Chironomus polytene chromosomes was reexamined. The in vivo effects of heat shock on chromosomal [3H]uridine labeling, RNA polymerase II distribution and ribonucleoprotein (RNP) formation were investigated. One primary result is a clarification of the number and location of chromosomal sites strongly induced by treatment at 37 degrees C for 60 min. In total, seven major heat shock loci were identified by transcription autoradiography in Chironomus tentans: I-20A, II-16B, II-10C, II-4B, II-1C, III-12B, and IV-5C. Secondly, combining immunofluorescence with transcription autoradiography, I find RNA polymerase II occurring after heat shock at multiple chromosomal sit…
Magnetic Resonance Microscopy Contribution to Interpret High-Resolution Magic Angle Spinning Metabolomic Data of Human Tumor Tissue
2010
[EN] HRMAS NMR is considered a valuable technique to obtain detailed metabolic profile of unprocessed tissues. To properly interpret the HRMAS metabolomic results, detailed information of the actual state of the sample inside the rotor is needed. MRM (Magnetic Resonance Microscopy) was applied for obtaining structural and spatially localized metabolic information of the samples inside the HRMAS rotors. The tissue was observed stuck to the rotor wall under the effect of HRMAS spinning. MRM spectroscopy showed a transference of metabolites from the tissue to the medium. The sample shape and the metabolite transfer after HRMAS indicated that tissue had undergone alterations and it can not be s…
Histological and ultrastructural study of corneal tunnel incisions using diamond and steel keratomes
1998
Abstract Purpose: To study the morphology of corneal tunnel incisions using diamond and steel keratomes. Setting: Department of Ophthalmology, University of Giessen, Germany. Methods: Corneal tunnel incisions were performed in six human cadaver eyes using three types of diamond keratomes and a steel keratome. The incision profile and morphology were evaluated and compared using light and scanning electron microscopy. Results: The steel keratome caused more disruption of corneal stromal tissue, while the diamond keratomes produced a more regular, smoother incision. The dissecting incision resulted in a smoother surface of cut stromal tissue than the stab incision. Conclusions: The high quali…