Search results for "Microscopy"
showing 10 items of 3390 documents
Endocytosis in skeletal muscle fibers.
1999
Defining the organization of endocytic pathway in multinucleated skeletal myofibers is crucial to understand the routing of membrane proteins, such as receptors and glucose transporters, through this system. Here we analyzed the organization of the endocytic trafficking pathways in isolated rat myofibers. We found that sarcolemmal-coated pits and transferrin receptors were concentrated in the I band areas. Fluid phase markers were taken up into vesicles in the same areas along the whole length of the fibers and were then delivered into structures around and between the nuclei. These markers also accumulated beneath the neuromuscular and myotendinous junctions. The recycling compartment, lab…
Organic salts and aromatic substrates in two-component gel phase formation: the study of properties and release processes
2015
To identify gel phases able to act as confined reaction media or materials for the removal of organic pollutants, we studied two-component gel phases formed by naphthalenedisulfonate diimidazolium salts in the presence of some organic guests, in 1-propanol solution. Guests differing in π-surface area, bulkiness and electronic properties were taken into account. Soft materials obtained were investigated for their thermal stability, self-repairing ability and morphology. Furthermore, two-component gel phase formation was studied using resonance light scattering (RLS) measurements. Guest release processes from the gel phase were also studied. These processes were monitored as a function of tim…
Characterizing Low-Permeable Granitic Rock from Micrometer to Centimeter Scale: X-ray Microcomputed Tomography, Confocal Laser Scanning Microscopy an…
2006
AbstractFirst results of combining X-ray microcomputer tomography (µCT), confocal laser-scanning microscopy (CLSM) and 14C-polymethylmethacrylate (14C-PMMA) impregnation techniques in the study of granitic rock samples are reported. Combining results of µCT and CLSM with those of the 14C-PMMA technique, the mineral-specific porosity and morphology of the open pore space, as well as its connectivity, could be analyzed from a micrometer up to a decimeter scale.Three different types of granite were studied. In two cases part of the micro-fissure and pore apertures were found to be in a micrometer scale, but in one case all grain-boundary openings were below the detection limit. Micrometer-scal…
The influence of the raw material and production method on the morphology, microstructure, chemical and phase composition of tetrabasic lead sulfate …
1992
Abstract Tetrabasic lead sulfate (4BS) produced by two different methods (solution and paste) and from two different raw materials (tetragonal PbO and leady oxide) were charaterized by different analytical techniques. The techniques applied were wet-chemical methods, scanning electron microscopy, transmission electron microscopy and X-ray powder diffraction. The preparation method was observed to be the most decisive factor influencing the structure and the morphology of the 4BS pastes in this study.
Correlations between transmural mechanical and morphological properties in porcine thoracic descending aorta.
2015
Determination of correlations between transmural mechanical and morphological properties of aorta would provide a quantitative baseline for assessment of preventive and therapeutic strategies for aortic injuries and diseases. A multimodal and multidisciplinary approach was adopted to characterize the transmural morphological properties of descending porcine aorta. Histology and multi-photon microscopy were used for describing the media layer micro-architecture in the circumferential-radial plane, and Fourier Transform infrared imaging spectroscopy was utilized for determining structural protein, and total protein content. The distributions of these quantified properties across the media thi…
Effect of the nanotube aspect ratio and surface functionalization on the morphology and properties of multiwalled carbon nanotube polyamide-based fib…
2013
In this study, the effect of the carbon nanotube (CNT) aspect ratio and surface functionalization on the mechanical behavior and morphological changes of polyamide (PA)-based fibers was investigated. Composites were prepared by the melt blending of CNTs with PA, and at a later time, the fibers were prepared by melt spinning and cold drawing. A reinforcement effect was noticed for all of the CNTs samples, and the increase in the mechanical properties and dimensional stability was more pronounced for highly oriented filaments. When the elongational flow was increased, the orientation of CNTs along the fiber direction was observed, but the nanotube alignment was much more difficult for CNTs wi…
Speckle-based configuration for simultaneous in vitro inspection of mechanical contractions of cardiac myocyte cells.
2013
An optical lensless configuration for a remote noncontact measuring of mechanical contractions of a vast number of cardiac myocytes is proposed. All the myocytes were taken from rats, and the measurements were done in an in vitro mode. The optical method is based on temporal analysis of secondary reflected speckle patterns generated in lensless microscope configuration. The processing involves analyzing the movement and the change in the statistics of the secondary speckle patterns that are created on top of the cell culture when it is illuminated by a spot of laser beam. The main advantage of the proposed system is the ability to measure many cells simultaneously (∼1000 cells) and to extra…
Distribution of Cytoglobin in the Mouse Brain
2016
Cytoglobin (Cygb) is a vertebrate globin with so far poorly defined function. It is expressed in the fibroblast cell-lineage but has also been found in neurons. Here we provide, using immunohistochemistry, a detailed study on the distribution of Cygb in the mouse brain. While Cygb is a cytoplasmic protein in active cells of the supportive tissue, in neurons it is located in the cytoplasm and the nucleus. We found the expression of Cygb in all brain regions, although only a fraction of the neurons was Cygb-positive. Signals were of different intensity ranging from faint to very intense. Telencephalic neurons in all laminae of the cerebral cortex (CCo), in the olfactory bulb (in particular pe…
The monocyte-macrophage system is affected in lysosomal storage diseases: an immunoelectron microscopic study
1997
Studying peripheral blood mononuclear cells (PBMCs) has become an important diagnostic tool in lysosomal storage diseases. Previous studies revealed that B and subclasses of T lymphocytes participate in the storage process, whereas the role of circulating monocytes was not clear. In this study, the involvement of CD14+ monocytes in lysosomal diseases was investigated. Blood samples from six patients with different lysosomal storage disorders were studied, including one with late--infantile and three with juvenile neuronal ceroid--lipofuscinoses, and two with mucopolysaccharidosis type VI. CD14+ cells were separated immunomagnetically from PBMCs and studied by light and electron microscopy. …
Synthesis and characterization of indium oxide at high pressures
2018
Introducción: La naturaleza es sorprendente pero a la vez limitada. A mi entender, nada tiene más potencial que aplicar el ingenio humano para modificar lo que nos rodea y crear algo completamente nuevo. La Física de la Materia Condensada es un campo que actualmente está ganando importancia en la Física moderna. En virtud de los éxitos logrados en Física de la Materia Condensada se han producido enormes avances en el campo de la electrónica cuántica, de los semiconductores y de la ciencia de materiales, teniendo como resultado numerosas aplicaciones tecnológicas que han cambiado nuestras vidas drásticamente en los últimos 50 años. Una de las ramas de la Física de la Materia Condensada es el…