Search results for "Minas"

Effects of Pulp and Paper Mill Effluent (BKME) on Physiology and Biochemistry of the Roach (Rutilus rutilus L.)

1996

The effects of bleached kraft pulp and paper mill effluent (BKME) on the roach (Rutilus rutilus L.) were studied under experimental and natural field conditions. In the acute experiment (72 h exposure to the concentrated BKME), the roach suffered from a general stress syndrome, characterized by a significant increase of cortisol and blood glucose, as well as a significant decrease of leucocrit and total plasma protein. In three weeks' exposure in a polluted and an unpolluted lake and in fish caught from the same lakes, the more specific effects of BKME treatments appeared. During the three weeks' exposure, slight hyperglycaemia as well as a decrease in a transaminase activity (GPT) and incr…

Oxidative Stress And Ubiquitin Ligases: Their Involvement In Alzheimer’s Disease Pathophysiology

2015

Oxidative stress is a major hallmark in Alzheimer’s Disease. We showed that amyloid beta (Aβ 1-42 ), induces mitochondrial oxidative stress. We focused on dysregulations of ubiquitin ligases in Alzheimer’s and their relation to oxidative stress. The anaphase-promoting complex/cyclosome (APC/C)-Cdh1 ubiquitin ligase has a role as cell cycle regulator in proliferating cells and, recently another role in the regulation the degradation of key glycolytic enzyme 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase-3 has been found (Almeida et al., 2012). Herrero-Mendez et al. observed in 2009 that inhibition of Cdh1 leads to an upregulation of Pfkfb3 in neurons and that this results in the activ…

Effect of substituted phenols on transaminase activity in the fish,Leuciscus idus melanotus L.

1986

Messung der gesamtaktivität von glutamat-oxalacetat-transaminase in leberhomogenaten

1971

Abstract A method is described, suitable for routine measurements of the total glutamicoxaloacetic transaminase activity in a large number of liver samples by treatment of homogenates with the detergent Triton X-100. Comparison with other methods and with results of other investigators shows that this method is superior. Neither sonic treatment nor freezing and thawing leads to a complete liberation of the mitochondrial transaminase activity. A number of other enzyme activities can be assayed in Triton-treated homogenates, such as glutamic-pyruvic transaminase, glutamate dehydrogenase, NADP-isocitric dehydrogenase, lactate dehydrogenase, and malate dehy drogenase, β-Hydroxybutyrate dehydrog…

Analysis of kynurenine transaminase activity in Drosophila by high performance liquid chromatography

1991

Abstract A sensitive assay for kynurenine transaminase activity (E.C. 2.6.1.7) based on rapid separation of the reaction product by high performance liquid chromatography (HPLC) has been developed. Drosophila sordidula extracts have been assayed by this new method and this is the first time that kynurenine transaminase activity has been demonstrated in Drosophila . The method of assay developed can be extended to any other organism. Kynurenine and 3-hydroxykynurenine were both used as substrates, and they were transaminated to kynurenic acid and xanthruenic acid, respectively. HPLC is used to separate and quantitate these reaction products from all other components in the reaction mixture. …

Epidemiology and pathogenesis of celiac disease and non-celiac gluten (wheat) sensitivity

2021

Abstract While in the past, celiac disease (CD) was considered the only clinical entity caused by the ingestion of gluten-containing grains, now there is evidence that a spectrum of gluten-related disorders, including also wheat allergy and non-celiac gluten (wheat) sensitivity (NCGS/NCWS), exists. The prevalence of gluten-related disorders is rising, and increasing numbers of individuals are empirically trying a gluten-free diet for a variety of signs and symptoms. CD is a gluten-induced immune-mediated enteropathy characterized by a specific genetic genotype [human leukocyte antigen (HLA)-DQ2 and HLA-DQ8 genes] and autoantibodies (antitissue transglutaminase and antiendomysial). NCGS/NCWS…

Biosynthesis of xanthurenic acid 8-O-beta-D-glucoside in Drosophila. Characterization of the xanthurenic acid:UDP-glucosyltransferase activity.

1990

Xanthurenic acid 8-glucoside is a side metabolite of the tryptophan-xanthommatin pathway in Drosophila. From 3-hydroxykynurenine, two biosynthetic pathways can be envisaged, one via xanthurenic acid, and another via 3-O-glucoside of 3-hydroxykynurenine. In this report evidence is presented to show that the synthesis takes place via xanthurenic acid. (a) We have demonstrated that the Drosophila melanogaster vermilion purple mutant (unable to synthesize 3-hydroxykynurenine) synthesizes xanthurenic acid 8-glucoside when fed with xanthurenic acid; and (b) the activities required for its synthesis via xanthurenic acid have been found (3-hydroxykynurenine transaminase and xanthurenic acid:UDP-glu…

The amphipathic peptide mellitin as a tool to study the membrane-dependent activation of tissue transglutaminase

2001

The role of membrane phospholipids on the cross-linking activity of guinea pig liver (tissue) transglutaminase has been investigated using the amphipathic model peptide melittin as glutaminyl substrate and the primary amine monodansylcadaverine as extrinsic amine donor. A marked increase of transglutaminase catalytic activity was observedin vitro assays in the presence of neutral membrane phospholipids. In contrast, activation was abolished in the presence of membranes containing pure anionic lipids. Enzyme activation could be ascribed to a direct binding of the lipid to the protein as demonstrated in enzymatic assays using a non membrane-interacting peptide (Cbz-Gln-Gly). The data obtained…

Zur Frage des Verhaltens der Glutamins�ure-Brenztraubens�ure-Transaminase im Blut-und Hautblasenserum bei Ekzem und bull�sen Dermatosen

1959

Vergleichende Untersuchungen der Glutaminsaure-Brenztraubensaure-Transaminase im Blutserum und dem Serum von Spontan-und Cantharidinblasen zeigten Aktivitatsunterschiede, die keinen Zusammenhang mit dem jeweiligen Hautprozes erkennen lassen. Die in Blut und Hautblasenflussigkeits oftmals nur wenig differenten Enzymaktivitaten dieser Aminopherase sprechen eher gegen als fur die Annahme ihrer Entstehung in menschlicher Epidermis.

Estudio de seguimiento de los niveles de vitamina d en pacientes con lupus eritematoso cutáneo y correlación con la severidad de la enfermedad

2014

INTRODUCCIÓN: La fuente más importante de vitamina D es la exposición de la piel a la radiación ultravioleta. Se han encontrado niveles bajos de vitamina D en pacientes con enfermedades autoinmunes, incluyendo diabetes tipo I, artritis reumatoide, esclerosis múltiple y lupus eritematoso sistémico. El lupus eritematoso cutáneo (LEC) se exacerba por la exposición solar y no existen estudios sobre prevalencia del déficit de vitamina D en los pacientes con LEC. Asimismo, se ha encontrado relación entre los niveles bajos de vitamina D y la prevalencia de enfermedades cardiovasculares. Esta asociación podría deberse a una relación entre los niveles de vitamina D y de lípidos en sangre. Llevamos a…