Search results for "Minor"
showing 10 items of 877 documents
Depletion of Alloreactive Donor T Lymphocytes by CD95-Mediated Activation-Induced Cell Death Retains Antileukemic, Antiviral, and Immunoregulatory T …
2007
In allogeneic hematopoietic stem cell transplantation (AHSCT) graft-versus-host disease (GVHD) and graft-versus-leukemia (GVL) effect are closely but not invariably linked. Thus, harnessing donor lymphocyte mediated GVL immunity and separating it from GVHD is of particular interest. Based on results obtained in murine models we have explored the CD95-mediated activation-induced cell death (AICD) strategy to selectively deplete alloreactivity in human donor T lymphocytes in vitro. Following stimulation of CD3(+) T cells isolated from HLA-A* 0201-positive donors with HLA or minor histocompatibility antigen mismatched hematopoietic or nonhematopoietic cells in the presence of agonistic anti-CD…
Rapid identification of minor histocompatibility antigen HA-1 subtypes H and R using fluorescence-labeled oligonucleotides
2000
Acknowledgments: We thank Brigitte Schuch and Karola Schmidt for excellent technical assistance. This work was supported by a grant of the Deutsche Krebshilfe (Nr. 70-2427 and Nr. 70-2428). Abstract: Donor-recipient disparitiy of the minor histocompatibility antigen HA-1 is relevant for the development of graft-versus-host disease after HLA-matched sibling allogeneic bone marrow transplantation in HLA-A*0201-positive individuals. Two different alleles of HA-1 with a single amino acid polymorphism have been identified. Here we describe a time- and cost-efficient method for HA-1 typing of genomic DNA, using site-specific hybridization probes with the LightCycler. This method was compared with…
Allogeneic stem cell transplantation for renal cell carcinoma
2011
Allogeneic hematopoietic stem cell transplantation from a compatible donor has been utilized as adoptive immunotherapy in metastatic, cytokine-refractory renal cell carcinoma (RCC). Since the year 2000, several investigators have established that RCC is susceptible to a graft-versus-tumor effect: they reported that patients with renal cancer may have partial or complete disease responses, in the 20-40% range, after allogeneic transplantation following a reduced-intensity regimen. However, transplant-related mortality is still high in the 10-20% range, and responses are rarely durable. Experimental evidence suggests that donor-derived T cells and natural killer cells are the main mediators o…
Aminoquinolines: Fluorescent sensors to DNA – A minor groove probe. Experimental and in silico studies
2023
An aminoquinoline (AQ4) was developed and proven to be a new and efficient DNA minor groove fluorescent probe. The specificity for DNA minor groove was attested by comparing it with well-established DNA probes such as Hoechst stain, acridine orange, and ethidium bromide. AQ4 was similar to the Hoechst stain, a classic minor groove probe, and opposite to acridine orange and ethidium bromide, the typical intercalating probes. An advantage of AQ4 to the Hoechst stain was the higher fluorescent signal-to-noise ratio (+DNA/-DNA). The interaction with DNA leads to an exclusive fluorescent band centered at 590 nm. The red-shifted fluorescent band is associated with a new absorption band (490 nm), …
Consent to Treatment and Anamnesis as Problem of Communication with Minor Patients in Healthcare Decision-Making
2016
Abstract Lately lawyers and medical professionals pay more attention too the process of minor patient healthcare. The research shall address the issues of legal relationship between minor patients and medical professionals, consent to treatment of minor patients and communication problems including the scope of information which the doctor can provide to the minor patient’s relatives to protect themselves and patients. Legislation prescribes that the information provided by the medical professional to the minor patient must be not only easy-to-understand, but also be consistent with the patient’s age maturity. However, in Latvia, for example, there are no guidelines that specify how medical…
De la protesta a las propuesta
2002
Nuove relazioni tra tessuto urbano e agricolo nel Parco del Gugliotta a Piano Tavola a Carini
2014
A sud-ovest della città di Palermo, lungo la fascia a nord-est dellʼabitato di Carini, segnata da monte Colombrina e attraversata dal torrente Gugliotta, si estende tra città e campagna unʼampia zona, denominata Piano Tavola. Elementi differenti ne costituiscono i margini: la frangia urbana dei volumi che si attestano sullʼasse di corso Italia (a ovest), lʼuliveto sotto Pizzo Castellaccio (a est), la villa Dominici (a nord-est), lʼarea industriale e il tracciato ferroviario (a nord) e la cava in località Manostalla (a sud-est). La lettura del paesaggio evidenzia la pressione esercitata dal costruito verso lʼuliveto e la campagna, la presenza di strade che cingono il quartiere isolandolo e l…
"Table 18" of "Energy dependence of event shapes and of alpha(s) at LEP-2."
1999
Distribution of Thrust Minor (MINOR) at cm energy 183 GeV.
Nota a Cass. 20 settembre 2019, n. 43542, C., in materia di atti osceni in luogo pubblico
2020
La sentenza in epigrafe si segnala all’attenzione perché chiarisce i presupposti di incriminazione del reato di atti osceni alla luce del d.lgs. 15 gennaio 2016, n. 8. Com’è noto, con tale intervento di riforma (e in particolare attraverso l’art. 2, co. 1, lett. a) del predetto decreto) il legislatore ha depenalizzato la forma “semplice” di atti osceni in luogo pubblico o aperto al pubblico, tuttora disciplinata dall’art. 527, comma 1, c.p., ma adesso soggetta a una “mera” sanzione amministrativa pecuniaria (pur se di ammontare notevole, trattandosi di una somma da euro 5.000 a euro 30.000). Resta invece la natura delittuosa degli atti osceni commessi «all’interno o nelle immediate vicinanz…
Eye tracking analysis of minor details in films for audio description
2012
This article focuses on the many instances when minute details found in feature films may have direct implications upon the development of both the visual and plot narratives. The main question we would like to ask examines whether very subtle details which may easily go unnoticed by the viewer should be audio described. To assess the visual consciousness of such minute details, a perception experiment was conducted using eye-tracking technology and questionnaires. Though the result is not conclusive, it shows a clear methodological approach in the field of the audio description of visual details, and does give some indication as to what should be taken into consideration in future studies …