Search results for "Moduli"
showing 10 items of 206 documents
A bacterial metabolite, trimethylamine N-oxide, disrupts the hemostasis balance in human primary endothelial cells but no coagulopathy in mice
2019
: The gut microbial metabolite, trimethylamine N-oxide (TMAO), was previously reported to induce platelet hypersensitivity, which leads to thrombotic risk. However, the molecular mechanism underlying the effects of TMAO on endothelial cells (EC), which is the primary vessel wall contact with the lumen, remains unclear. Here, we investigated the impact of TMAO on procoagulant activity (PCA) in EC and mice, for a possible link between microbiota and coagulation. To test the PCA of TMAO in EC, we performed one-stage clotting assays and converted into PCA. Antitissue factor (TF) antibody was used to test the TF role in PCA. Quantitative PCR was performed to measure the TF, thrombomodulin, IL-6,…
Ethylene modulates gene expression in cells of the marine sponge Suberites domuncula and reduces the degree of apoptosis.
1999
Sponges (phylum Porifera) live in an aqueous milieu that contains dissolved organic carbon. This is degraded photochemically by ultraviolet radiation to alkenes, particularly to ethylene. This study demonstrates that sponge cells (here the demosponge Suberites domuncula has been used), which have assembled to primmorphs, react to 5 microM ethylene with a significant up-regulation of intracellular Ca(2+) concentration and with a reduction of starvation-induced apoptosis. In primmorphs from S. domuncula the expression of two genes is up-regulated after exposure to ethylene. The cDNA of the first gene (SDERR) isolated from S. domuncula encodes a potential ethylene-responsive protein, termed ER…
Histamine Upregulates Gene Expression of Endothelial Nitric Oxide Synthase in Human Vascular Endothelial Cells
2003
Background— Histamine has a short-term, transient, stimulating effect on endothelial nitric oxide synthase (eNOS) activity; however, long-term effects on eNOS have not been described yet. In addition, the vascular effect of histamine seems to depend critically on eNOS functionality. Therefore, we studied the effects of histamine on eNOS gene expression and function. Methods and Results— In human umbilical vein endothelial cells (HUVECs) and HUVEC-derived EA.hy 926 cells, histamine upregulated eNOS mRNA (RNase protection assay) and protein (electron microscopic immunocytochemistry) expression. The upregulation of eNOS could be prevented by mepyramine, a selective antagonist at the H 1 recep…
Estudio funcional de polimorfismos de la vía de activación de la proteína C
2017
La trombosis venosa es una enfermedad multifactorial, originada por una suma de factores endógenos que predisponen al evento trombótico bajo la influencia de una exposición a factores exógenos. Dicha predisposición engloba factores de riesgo adquiridos que provocan en el individuo una disminución de su capacidad para enfrentarse a las alteraciones normales de la hemostasia, producto de la edad, dislipemias, diabetes, embarazo o puerperio, inmovilizaciones, intervenciones quirúrgicas, uso de píldoras anticonceptivas, tratamientos hormonales, etc. Además, se han identificado diferentes factores de riesgo genéticos, tales como las mutaciones factor V Leiden y protrombina G20210A, y el déficit …
Development of UWB localization network modules and integration in MansOS operating system
2021
Slimību pārnese starp cilvēkiem mūsdienās ir ļoti aktuāla problēma. Ir nepieciešams rast risinājumus šīs problēmas novēršanai un vai seku mīkstināšanai. Tādēļ šī bakalaura darba ietvaros tika optimizēti lokalizācijas sistēmu tīkli priekš cilvēku kustību pārraudzīšanas telpās, izmantojot pieejamas lokalizācijas sistēmas, kuras izmanto UWB tehnoloģiju un jau iepriekš izveidotu lokalizācijas sistēmu arhitektūru. Problēmas risinājums tika veidots valsts pētījumu programmas (VPP) projekta “Multidisciplināra pieeja COVID19 un citu nākotnes epidēmiju monitorēšanai, kontrolei un ierobežošanai Latvijā” ietvaros. Tika izveidotas divas lokalizāciju sistēmu arhitektūras, kuras tika izmantotas testēšana…
rhoB encoding a UV-inducible Ras-related small GTP-binding protein is regulated by GTPases of the Rho family and independent of JNK, ERK, and p38 MAP…
1998
The small GTPase RhoB is immediate-early inducible by DNA damaging treatments and thus part of the early response of eukaryotic cells to genotoxic stress. To investigate the regulation of this cellular response, we isolated the gene for rhoB from a mouse genomic library. Sequence analysis of the rhoB gene showed that its coding region does not contain introns. The promoter region of rhoB harbors regulatory elements such as TATA, CAAT, and Sp1 boxes but not consensus sequences for AP-1, Elk-1, or c-Jun/ATF-2. The rhoB promoter was activated by UV irradiation, but not by 12-O-tetradecanoylphorbol-13-acetate treatment. rhoB promoter deletion constructs revealed a fragment of 0.17 kilobases in …
Some Moduli and Constants Related to Metric Fixed Point Theory
2001
Indeed, there are a lot of quantitative descriptions of geometrical properties of Banach spaces. The most common way for creating these descriptions, is to define a real function (a “modulus” depending on the Banach space under consideration, and from this define a suitable constant or coefficient closely related to this function. The moduli and/or the constants are attempts to get a better understanding about two things: The shape of the unit ball of a space, and The hidden relations between weak and strong convergence of sequences.
CRM sistēmas ieviešana uzņēmumā "North Technology Group"
2021
Bakalaura darba tēma ir “CRM sistēmas ieviešana uzņēmumā “North Technology Group””. Darba mērķis ir konstatēt klientu attiecību pārvaldības sistēmas integrēšanas nepieciešamību uzņēmumā un izstrādāt ieviešanas plānu. Pētījuma procesā bija izanalizētas vairākas integrēšanas iespējas. Rezultātā darba autore secināja, ka labāk ir izmantot integrācijas moduļus, jo tas ievērojami atvieglo un paātrina implementēšanas procesu. Kopējais bakalaura darba apjoms ir 60 lapaspuses. Darbā ir iekļautas 7 tabulas un 14 attēli.
Characterization of Membrane-Bound Cyclic Nucleotide Phosphodiesterases from Bovine Aortic Smooth Muscle
1992
This study reports the isolation and characterization of cyclic nucleotide phosphodiesterases (PDEs) associated with membrane fraction in comparison to cytosolic forms from bovine aorta. DEAE-Sephacel chromatography of a solubilized membrane fraction from a homogenate, prepared under isotonic conditions in the presence of protease inhibitors, yielded one major peak of PDE activity that specifically hydrolyzed cAMP and was not stimulated by calmodulin: It appeared to contain two subtypes of PDE. The first subtype belonged to the cyclic GMP (cGMP)-inhibited PDE family, (PDE III): It had an apparent Km value of 0.4 microM and was potently inhibited by cGMP, LY186126, and cilostamide. The secon…
Interaction of endothelial cells and neutrophilsin vitro: kinetics of thrombomodulin, intercellular adhesion molecule-1 (ICAM-1), E-selectin, and vas…
1999
SUMMARYRecently markers of endothelial cell activation or injury gained increasing interest as serological parameters of disease activation in vasculitides. Among these, soluble serum thrombomodulin, ICAM-1, VCAM-1 and E-selectin are of particular interest. However, only thrombomodulin showed the expected close correlation. The objective of this study was to investigate in vitro the kinetics of these endothelial cell receptors after interaction of unstimulated or cytokine-activated polymorphonuclear neutrophils (PMN) and endothelial cells in order to find evidence explaining these different clinical findings. Over the time period of up to 48 h of incubation the kinetics of thrombomodulin, I…