Search results for "Mono"

Composition of the essential oil ofPallenis spinosa (L.) Cass. (Asteraceae)

2003

The essential oil of Pallenis spinosa (L.) Cass. was obtained by hydrodistillation. In total 38 components were identified by GC and GC–MS. Oxygenated sesquiterpenoids amounted to 60.2% of the oil. The main components were germacra-1(10),5-dien-3,4-diol (18.4%), α-cadinol (14.1%), 3-acetoxygermacra-1(10),5-dien-4-ol (13.0%), T-cadinol (8.2%) and δ-cadinene (5.8%). The oil does not show antimicrobial activity. Copyright © 2003 John Wiley & Sons, Ltd.

Essential oil composition ofArtemisia parvi?ora aerial parts

2003

The chemical composition of the essential oil from aerial parts of A. parviflora was analysed by GC and GC–MS. Twenty-two compounds, accounting for 72.7% of the oil, were identified. The main components identified were β-caryophyllene (15.3%), germacrene D (14.7%), camphor (11.4%), artemisia ketone (7.8%), 1,8-cineole (5.8%), α-copaene (2.6%), artemisia alcohol (2.6%), terpinene-4-ol (2.3%), caryophyllene oxide (1.2%), α-pinene (1.1%), sabinyl acetate (1.1%) and α-humulene (1.1%). Copyright © 2003 John Wiley & Sons, Ltd.

Interrelationships of the Haploporinae (Digenea: Haploporidae): A molecular test of the taxonomic framework based on morphology

2009

The taxonomic framework of the Haploporidae is evaluated and the relationships within the Haploporinae are assessed for the first time at the generic level using molecular data. Partial 28S and complete ITS2 rDNA sequences from representatives of six of the nine recognised genera within the Haploporinae were analysed together with published sequences representing members of two haploporid subfamilies and of the closely related family Atractotrematidae. Molecular analyses revealed: (i) a close relationship between the Atractotrematidae and the Haploporidae; (ii) strong support for the monophyly of the Haploporinae, Dicrogaster and Saccocoelium, and the position of Ragaia within the Haplopori…

M22 based (manual) ELISA for TSH-receptor antibody (TRAb) measurement is more sensitive than 2nd generation TRAb assays

2009

In Vitro Effects of Antiphospholipid Syndrome-IgG Fractions and Human Monoclonal Antiphospholipid IgG Antibody on Human Umbilical Vein Endothelial Ce…

2009

It has been shown that stimulation of endothelial cells and monocytes by antiphospholipid antibodies leads to a prothrombotic state involving upregulation of tissue factor (TF). We examined the in vitro effects of IgG fractions from patients with antiphospholipid syndrome (APS) and of a β-2-glycoprotein 1-independent human monoclonal antiphospholipid antibody (HL-5B) on human umbilical vein endothelial cells (HUVEC) in comparison to untreated cell controls and to exposure to monoclonal IgG control antibody. We also examined the effect of recombinant monocyte chemoattractant protein-1 (MCP-1) on peripheral blood monocytes. Stimulation of endothelial cells with APS IgG fractions or HL-5B resu…

OZONE THERAPY: MECHANISMS OF ACTION, RECENT LITERATURE AND NEWLY DISCOVERED BIOCHEMICAL PATHWAYS

2018

Ozone therapy is widely used in many countries since many years. Recently, the increasing widespread of this complementary therapy has been accomplished by an increased number of basic and clinic papers published on international journals. This lecture will deal on the first approach by a pharmacological point of view in the aim to characterize the mechanisms activated at sub-cellular level by ozone when used for medical and beauty application at low graded doses. The first theory was based upon the fact that the exposure to low, non-toxic, ozone concentrations could increase the efficacy of the endogenous antioxidant system by increasing the production or the activity of some enzymes exert…

Rituximab modulates the expression of IL-22 in the salivary glands of patients with primary Sjogren's syndrome

2012

We have recently demonstrated that interleukin (IL)-22, mainly produced by T-helper 17 effector cells, natural killer (NK)p44+NK cells and epithelial cells, may be potentially involved in the pathogenesis of primary Sjogren's syndrome (pSS).1 The IL-22/IL-22R pathway is known to play a role in the emergence of T and B-cell lymphoma2 ,3 and pSS is considered a risk factor for the development of lymphoma.4 Rituximab, which has historically been used for the treatment of B-cell lymphoma,5 has also been considered to be effective in the therapy of pSS.6 Ten consecutive patients with pSS (eight women and two men, with a mean duration of disease of 48±18 months), diagnosed according to the Americ…

Rôle de l’inflammasome dans les pathologies cardiovasculaires

2011

NOD-like receptors (NLRs) constitute a recently identified family of intracellular pattern recognition receptors which contains more than 20 members in mammals. Some of the NLRs, the NALP subfamily, constituted from 14 members, many of them without actual identified role, form multiproteic complex known as inflammasome, that initiate inflammation by processing inactive pro-caspase-1 to its active form, allowing the cleavage and subsequent activation of pro-IL-1β and pro-IL-18. We review the identified roles of NLRs in pathologies and argue for the role of inflammasome in the development of cardiovascular diseases. The atherogenic cytokines IL-1β and IL-18 are matured in NLRPs inflammasomes.…

In Vitro Stimulation and Expansion of Human Tumour-Reactive CD8+ Cytotoxic T Lymphocytes by Anti-CD3/CD28/CD137 Magnetic Beads

2011

Adoptive immunotherapy with tumour-reactive CD8(+) cytotoxic T lymphocytes (CTLs) requires efficient in vitro approaches allowing the expansion of CTLs to large numbers prior infusion. Here, we investigated the antigen-independent activation and the expansion of human T cells in peripheral blood mononuclear cells (PBMCs) and in tumour-reactive CTLs using Dynabeads coated with monoclonal antibodies to CD3 and to the costimulatory molecules CD28 and CD137 (4-1BB). T cells in PBMCs showed an increased expansion rate of 15- to 17-fold during a 2-week culture period using antibody-conjugated beads with interleukin-2 (IL-2) added versus IL-2 alone. No significant difference between CD3/CD28 beads…

Molecular specification of cell lines in the embryo of Platynereis (Annelida)

1992

In this study we describe the site and moment of histospecific differentiation in developmental stages of the annelid Platynereis dumerilii by use of biochemical markers. The monoclonal antibody (mab) OI7 and uncloned hybridoma supernatants (pAb's) OI8, OI10, OI46 and OI69 recognize neural antigens that appear asynchronously during development. By an enzymatic test, acetylcholinesterase (AChE) was found specific for nervous tissue as well. The patterns of neural structures labelled by antibodies differ, however, from those revealed by AChE staining. Experimental inhibition of transcription (with actinomycin D) and of translation (using puromycin) demonstrate that the expression of histospec…