Search results for "Mono"

The effect of carvacrol on enteric viruses.

2014

Carvacrol, a monoterpenic phenol, is said to have extensive antimicrobial activity in a wide range of food spoilage or pathogenic fungi, yeast and bacteria. The aim of this study was to assess its antiviral activity on norovirus surrogates, feline calicivirus (FCV), murine norovirus (MNV), and hepatitis A virus (HAV), as well as its potential in food applications. Initially, different concentrations of carvacrol (0.25, 0.5, 1%) were individually mixed with each virus at titers of ca. 6-7 log TCID50/ml and incubated 2h at 37°C. Carvacrol at 0.5% completely inactivated the two norovirus surrogates, whereas 1% concentration was required to achieve ca. 1 log reduction of HAV. In lettuce wash wa…

Functional properties of a monoclonal antibody inhibiting the hepatitis C virus RNA-dependent RNA polymerase.

2001

The hepatitis C virus (HCV) RNA-dependent RNA polymerase (RdRp), represented by nonstructural protein 5B (NS5B), has recently emerged as a promising target for antiviral intervention. Here, we describe the isolation, functional characterization, and molecular cloning of a monoclonal antibody (mAb) inhibiting the HCV RdRp. This mAb, designated 5B-12B7, binds with high affinity to a conformational epitope in the palm subdomain of the HCV RdRp and recognizes native NS5B expressed in the context of the entire HCV polyprotein or subgenomic replicons. Complete inhibition of RdRp activity in vitro was observed at equimolar concentrations of NS5B and mAb 5B-12B7, whereas RdRp activities of classica…

Translocation of the nuclear autoantigen La to the cell surface of herpes simplex virus type 1 infected cells.

1992

Recently we developed a procedure to translocalize one of the extractable nuclear antigens (ENAs), the La protein, to the cell surface of CV-1 cells. Here we report that herpes simplex virus type 1 infection can also induce a translocation of the autoantigen to the cell surface. On the cell surface we detected La protein assembled with large protrusions. Within these protrusions La protein colocalized with virus particles. These protrusions are known to be released from the cell after virus infections. Such complexes consisting of self and virus could provide helper determinants for an anti-self response, and therefore be important in generation of autoimmunity.

Rapid and sensitive detection of metapneumovirus in clinical specimens by indirect fluorescence assay using a monoclonal antibody.

2008

Human metapneumovirus, with two known genotypes named A and B, is associated with mild respiratory symptoms to severe LRTI in children, high-risk adults and the elderly. Rapid and reliable methods of hMPV detection in clinical samples are essential to implement appropriate care, to better understand the pathology of hMPV and to determine its epidemiology. Respiratory samples from 1,386 patients collected during 2 consecutive years were screened for hMPV using indirect immunofluorescence (IFA) assay with a monoclonal antibody. Forty-three patients tested positive for hMPV by the IFA method. In parallel, the samples were examined with RT-PCR on the F gene. Of these, 41 specimens were RT-PCR p…

Correlazione fra deficit di vit.D monoidrossilata e danno epatico in soggetti talassemici adulti

2002

MELNBALTĀ FOTO IZMANTOŠANAS KALENDĀRU DIZAINĀ VIZUĀLI STILISTISKIE ASPEKTI

2019

Diplomdarba “Melnbaltā foto izmantošanas kalendāru dizainā vizuāli stilistiskie aspekti” teorētiskajā daļā autore pēta fotogrāfijas vēsturi, melnbaltās fotogrāfijas izveides principus, kalendāru vēsturi un to veidus. Darba pētnieciskajā daļā pēc vairākiem kritērijiem tiek analizēti 21. gadsimta melnbalto fotokalendāru dizainparaugi no Latvijas un visas pasaules. Darba radošajā daļā tiek izveidots praktiski pielietojams melnbalts foto kalendārs ar kaķu portretiem. Darbs sastāv no 77 lappusēm, 68 attēliem un 1. tabulas. Tā izveidē izmantoti 62 avoti.

Vīriešu kimono aktualitāte Japānā 21. gadsimtā

2019

Maģistra darba nosaukums ir Vīriešu kimono aktualitāte Japānā 21. gadsimtā. Šī darba mērķis ir izpētīt vīriešu kimono nēsāšanas tradīcijas dažādos Japānas laikmetos un tā un attīstības gaitu, analizējot iemeslus, kāpēc mūsdienās kimono sastopamība vīriešu vidū ir ievērojami samazinājusies, salīdzinot ar sievietēm. Būtiska darba daļa veltīta vīriešu kimono tipoloģijai, izvēršot tā veidus un fiksējot to simboliku, kas skatīta ietērpa aktualitātē un sastopamībā mūslaikos. Pētnieciskā darba izstrādes laikā veikta aptauja, anketējot 100 japāņu vīriešus saistībā ar viņu kimono nēsāšanas paradumiem un relevanci mūsdienās. Iegūtie rezultāti ļāvuši deducēt japāņu tradicionālā ietērpa aktualitāti mūs…

The wage curve and local monopsony power

2019

AbstractUsing longitudinal micro-data from Finland, a country with a geographically dispersed population and relatively long distances between local labor markets, this paper examines the responsiveness of the pay level to local unemployment conditions. In particular, this study tests the hypothesis that the pay level is more responsive to the unemployment level in less agglomerated and more remote regions as might be expected if employers have a higher degree of monopsony power in such regions. The results consistently suggest that the pay level is lower in localities with a higher unemployment level and, hence, provide strong support for the so-called wage curve hypothesis, which predicts…

Development of an immunochromatographic assay based on carbon nanoparticles for the determination of the phytoregulator forchlorfenuron

2013

Rapid analytical methods enabling the determination of diverse targets are essential in a number of research areas, from clinical diagnostics to feed and food quality and safety. Herein, the development of a quantitative immunochromatographic assay for the detection of the synthetic phytoregulator forchlorfenuron (CPPU) is described. The competitive lateral flow immunoassay (LFIA) was based on the immobilization onto a nitrocellulose membrane of an ovalbumin-CPPU conjugate (test line) and on the use of an immunodetection ligand consisting of carbon nanoparticles labeled with an anti-CPPU monoclonal antibody through interaction with a secondary antibody. The presence of CPPU in horticultural…

On the convergence of zero-point vibrational corrections to nuclear shieldings and shielding anisotropies towards the complete basis set limit in wat…

2016

The method and basis set dependence of zero-point vibrational corrections (ZPVCs) to nuclear magnetic resonance shielding constants and anisotropies has been investigated using water as a test system. A systematic comparison has been made using the Hartree–Fock, second-order Møller–Plesset perturbation theory (MP2), coupled cluster singles and doubles (CCSD), coupled cluster singles and doubles with perturbative triples corrections (CCSD(T)) and Kohn–Sham density functional theory with the B3LYP exchange-correlation functional methods in combination with the second-order vibrational perturbation theory (VPT2) approach for the vibrational corrections. As basis sets, the correlation consisten…