Search results for "Monolithic HPLC column"
showing 2 items of 32 documents
The effect of the pore structure and zeta potential of porous polymer monoliths on separation performance in ion-exchange mode.
2007
Most often, in bioseparations involving charged macromolecules, the chromatographic systems have low Reynolds and high Peclet numbers. For such systems, an expression is developed and presented in this work for evaluating the throughput in polymeric monoliths where ion-exchange adsorption occurs, as a function of (i) the pressure drop along the length of the monolith, (ii) the functional form and width of the throughpore-size distribution of the monolith, and (iii) the magnitude of the zeta potential on the surface of the throughpores of the monolith. Gaussian and log-normal throughpore-size distributions whose mean throughpore-size and standard deviation values are based on experimentally …
Photografted methacrylate-based monolithic columns coated with cellulose tris(3,5-dimethylphenylcarbamate) for chiral separation in CEC
2018
A chiral capillary monolithic column for enantiomer separation in capillary electrochromatography was prepared by coating cellulose tris(3,5-dimethylphenylcarbamate) on porous glycidyl methacrylate-co-ethylene dimethacrylate monolith in capillary format grafted with chains of [2(methacryloyloxy)ethyl] trimethylammonium chloride. The surface modification of the monolith by the photografting of [2(methacryloyloxy)ethyl] trimethylammonium chloride monomer as well as the coating conditions of cellulose tris(3,5-dimethylphenylcarbamate) onto the grafted monolithic scaffold were optimized to obtain a stable and reproducible chiral stationary phase for capillary electrochromatography. The effect o…