Search results for "Motet"
showing 9 items of 9 documents
2019
Integral membrane proteins of the aquaporin family facilitate rapid water flux across cellular membranes in all domains of life. Although the water-conducting pore is clearly defined in an aquaporin monomer, all aquaporins assemble into stable tetramers. In order to investigate the role of protomer–protomer interactions, we analyzed the activity of heterotetramers containing increasing fractions of mutated monomers, which have an impaired oligomerization propensity and activity. In order to enforce interaction between the protomers, we designed and analyzed a genetically fused homotetramer of GlpF, the aquaglyceroporin of the bacterium Escherichia coli (E. coli). However, increasing fractio…
A rhamnose-binding lectin from sea bass (Dicentrarchus labrax) plasma agglutinates and opsonizes pathogenic bacteria
2014
Abstract The discovery of rhamnose-binding lectins (RBLs) in teleost fish eggs led to the identification of a novel lectin family characterized by a unique sequence motif and a structural fold, and initially proposed to modulate fertilization. Further studies of the RBL tissue localization and gene organization were also suggestive of role(s) in innate immunity. Here we describe the purification, and biochemical and functional characterization of a novel RBL (DlRBL) from sea bass (Dicentrarchus labrax) serum. The purified DlRBL had electrophoretic mobilities corresponding to 24 kDa and 100 kDa under reducing and non-reducing conditions, respectively, suggesting that in plasma the DlRBL is p…
Voce “Il Verso, Antonio”
2018
Biotin Induces Tetramerization of a Recombinant Monomeric Avidin
2001
Chicken avidin, a homotetramer that binds four molecules of biotin was converted to a monomeric form by successive mutations of interface residues to alanine. The major contribution to monomer formation was the mutation of two aspartic acid residues, which together account for ten hydrogen bonding interactions at the 1-4 interface. Mutation of these residues, together with the three hydrophobic residues at the 1-3 interface, led to stable monomer formation in the absence of biotin. Upon addition of biotin, the monomeric avidin reassociated to the tetramer, which exhibited properties similar to those of native avidin, with respect to biotin binding, thermostability, and protease resistance. …
Geometrie della natura. Matematica ed epistemologia
2004
Sismotettonica del Margine Continentale della Sicilia nord-occidentale: implicazioni per la valutazione del geohazard
2017
I processi tettonici attivi nel margine continentale della Sicilia settentrionale (MCSS) sono stati analizzati per la realizzazione della carta sismotettonica, al fine di ottenere uno strumento utile per la valutazione della pericolosità sismica della regione. La carta sismotettonica è realizzata attraverso la sovrapposizione di layer che rappresentano la distribuzione di tematismi, quali litostratigrafia, tettonica, sismicità, flussi di calore, gravimetria, magnetometria, profondità della Moho, movimenti orizzontali e verticali, frane, fuoriuscite di fluidi, evidenziando la relazione tra questi e le strutture attive. Il MCSS si trova in una zona di transizione tra la catena siciliano-maghr…