Search results for "Motif"
showing 10 items of 290 documents
Cytochrome-P450 phosphorylation as a functional switch
2002
Xenobiotic metabolizing cytochromes P450 (CYP) were shown to be phosphorylated in vitro (using purified protein kinases together with purified CYPs), in intact cells (in V79 cells after transfection of cDNAs coding for individual CYPs, in diagnostic mutants, in hepatocytes), and in whole organisms (rats). CYP phosphorylation is highly isoenzyme selective in that only some CYPs are phosphorylated. Protein kinase A (PKA) was identified as a major catalyst for the phosphorylation of CYPs. The PKA recognition motif Arg-Arg-X-Ser is present in several members of the CYP2 family, but is used by only some of them, most notably by CYP2B1/2B2 and CYP2E1. For CYP2B1 it was shown that a substantial po…
The transformation of the Horatian Sabine farm in Persius? Satire 6
2020
The purpose of this article is to focus on the motif of the countryside in Roman satire. In particular, it aspires to study the way the Sabine farm is delineated in Horace's Satire 2.6 and later in his Epistles, in order to elucidate the evolution of this motif in Persius? 6th Satire. In this poem, Persius addresses Caesius Bassus, who retreats to Sabine to seek poetic inspiration, but seems trapped in the paths of earlier poetry and cannot attain bliss, while the poet achieves it in the port of Luna.
F-Type Lectins: A highly diversified family of fucose-binding proteins with a unique sequence motif and structural fold, involved in self/non-self-re…
2017
The F-type lectin (FTL) family is one of the most recent to be identified and structurally characterized. Members of the FTL family are characterized by a fucose recognition domain [F-type lectin domain (FTLD)] that displays a novel jellyroll fold (“F-type” fold) and unique carbohydrate- and calcium-binding sequence motifs. This novel lectin family comprises widely distributed proteins exhibiting single, double, or greater multiples of the FTLD, either tandemly arrayed or combined with other structurally and functionally distinct domains, yielding lectin subunits of pleiotropic properties even within a single species. Furthermore, the extraordinary variability of FTL sequences (isoforms) th…
Prediction of Specific TCR-Peptide Binding From Large Dictionaries of TCR-Peptide Pairs
2019
Abstract The T cell repertoire is composed of T cell receptors (TCR) selected by their cognate MHC-peptides and naive TCR that do not bind known peptides. While the task of distinguishing a peptide-binding TCR from a naive TCR unlikely to bind any peptide can be performed using sequence motifs, distinguishing between TCRs binding different peptides requires more advanced methods. Such a prediction is the key for using TCR repertoires as disease-specific biomarkers. We here used large scale TCR-peptide dictionaries with state-of-the-art natural language processing (NLP) methods to produce ERGO (pEptide tcR matchinG predictiOn), a highly specific classifier to predict which TCR binds to which…
Semblanza iconográfica de la realeza sapiencial de Alfonso X: las miniaturas liminares de los códices regios
2016
La producción libraria de Alfonso X testimonia la simbiosis perfecta entre las funciones de gobierno y la labor intelectual y sistematiza su ideario político y cultural, no solo en texto, sino también en imagen. A partir de las miniaturas liminares de los códices regios, se evidencia que el motivo icónico privilegiado en la representación personal de Alfonso X es el rey con el libro: se analizan las distintas variantes de este modelo iconográfico que estampa la amplia gama de atribuciones que conforman el concepto de autoría alfonsí y consolida el modelo de la realeza sapiencial castellana.
cis-regulatory variation modulates susceptibility to enteric infection in the Drosophila genetic reference panel
2020
Abstract Background Resistance to enteric pathogens is a complex trait at the crossroads of multiple biological processes. We have previously shown in the Drosophila Genetic Reference Panel (DGRP) that resistance to infection is highly heritable, but our understanding of how the effects of genetic variants affect different molecular mechanisms to determine gut immunocompetence is still limited. Results To address this, we perform a systems genetics analysis of the gut transcriptomes from 38 DGRP lines that were orally infected with Pseudomonas entomophila. We identify a large number of condition-specific, expression quantitative trait loci (local-eQTLs) with infection-specific ones located …
Role of RNA Motifs in RNA Interaction with Membrane Lipid Rafts: Implications for Therapeutic Applications of Exosomal RNAs
2021
RNA motifs may promote interactions with exosomes (EXO-motifs) and lipid rafts (RAFT-motifs) that are enriched in exosomal membranes. These interactions can promote selective RNA loading into exosomes. We quantified the affinity between RNA aptamers containing various EXO- and RAFT-motifs and membrane lipid rafts in a liposome model of exosomes by determining the dissociation constants. Analysis of the secondary structure of RNA molecules provided data about the possible location of EXO- and RAFT-motifs within the RNA structure. The affinity of RNAs containing RAFT-motifs (UUGU, UCCC, CUCC, CCCU) and some EXO-motifs (CCCU, UCCU) to rafted liposomes is higher in comparison to aptamers withou…
Lejtmotyw Miłosierdzia Bożego w kulturze muzycznej od średniowiecza do współczesności
2017
Lejtmotyw miłosierdzia Bożego jest obecny w kulturze muzycznej wszystkich epok, od czasów średniowiecza, gdzie był obecny w śpiewach gregoriańskich, aż po czasy współczesne. Fundamentem śpiewów, powstałych na przestrzeni wieków, były najczęściej teksty biblijne sławiące tajemnicę miłosierdzia Bożego i Bożej łaskawości. Sposób wyrażenia lejtmotywu miłosierdzia Bożego zależał od zespolenia warstwy tekstu ze współczynnikami muzycznego stylu danej epoki, a także od duchowości i oblicza mistyki danej epoki. Obecnie jesteśmy świadkami powstania wielu kompozycji zawierających lejtmotyw miłosierdzia Bożego. Ma to związek z intensywnie rozwijającym się w ostatnich latach – zwłaszcza za pontyfikatu ś…
Motivic Pattern Extraction in Music, and Application to the Study of Tunisian Modal Music
2007
A new methodology for automated extraction of repeated patterns in time-series data is presented, aimed in particular at the analysis of musical sequences. The basic principles consists in a search for closed patterns in a multi-dimensional parametric space. It is shown that this basic mechanism needs to be articulated with a periodic pattern discovery system, implying therefore a strict chronological scanning of the time-series data. Thanks to this modelling global pattern filtering may be avoided and rich and highly pertinent results can be obtained. The modelling has been integrated in a collaborative pro ject between ethnomusicology, cognitive sciences and computer science, aimed at the…
New Melanocortin 1 Receptor Binding Motif Based on the C-Terminal Sequence of ?-Melanocyte-Stimulating Hormone
2006
The C-terminal tripeptide of the alpha-melanocyte stimulating hormone (alpha-MSH11-13) possesses strong antiinflammatory activity without known cellular target. In order to better understand the structural requirements for function of such motif, we designed, synthesized and tested out Trp- and Tyr-containing analogues of the alpha-MSH11-13. Seven alpha-MSH11-13 analogues were synthesized and characterized for their binding to the melanocortin receptors recombinantly expressed in insect (Sf9) cells, infected with baculovirus carrying corresponding MC receptor DNA. We also tested these analogues on B16-F1 mouse melanoma cells endogenously expressing the MC1 receptor for binding and for abili…