Search results for "Multiplex"
showing 10 items of 337 documents
Comparative analysis of short tandem repeats and single nucleotide polymorphisms on the Y-chromosome in Germans, Chinese and Thais.
2003
We have typed genomic DNA samples from 95 individuals from Western Germany, 78 individuals from Bangkok/Thailand and 56 individuals from Chengdu/China for 11 Y-chromosomal diallelic polymorphisms and eight short tandem repeat (STR) systems. For single nucleotide polymorphism (SNP) analysis, a rapid method was applied using the single base extension technology (minisequencing) in combination with capillary electrophoresis. PCR products for SRY-8299, Tat, SRY2627, 92R7, SRY1532, M9, M13, M17/M19 and M20 were pooled and used as templates for the commercially available SNaPshot kit. In addition to these ten SNPs we also tested the Y-chromosomal diallelic Alu repeat insertion DYS287 (YAP) by aga…
Semiquantitative multiplex PCR: a useful tool for large rearrangement screening and characterization
2006
Methods presently employed for detection of large rearrangements have several drawbacks, such as the amount of sample and time required, technical difficulty, or the probability of false-negative carriers. Using the low-density-lipoprotein receptor (LDLR) gene, whose mutations are responsible for familial hypercholesterolemia (FH), we have developed a procedure to detect large rearrangements in this gene based on semiquantitative PCR, with important improvements as compared to previous methods. Our method covers the complete LDLR gene and introduces an internal control in the reaction. The procedure discriminates the four different large rearrangements (two deletions and two insertions) tha…
A multiplex assay with 52 single nucleotide polymorphisms for human identification.
2006
A total of 52 SNPs reported to be polymorphic in European, Asian and African populations were selected. Of these, 42 were from the distal regions of each autosome (except chromosome 19). Nearly all selected SNPs were located at least 100 kb distant from known genes and commonly used STRs. We established a highly sensitive and reproducible SNP-typing method with amplification of all 52 DNA fragments in one PCR reaction followed by detection of the SNPs with two single base extension reactions analysed using CE. The amplicons ranged from 59 to 115 bp in length. Complete SNP profiles were obtained from 500 pg DNA. The 52 loci were efficiently amplified from degraded samples where previously on…
Spatial information transmission using orthogonal mutual coherence coding.
2005
We use the coherence of a light beam to encode spatial information. We apply this principle to obtain spatial superresolution in a limited aperture system. The method is based on shaping the mutual intensity function of the illumination beam in a set of orthogonal distributions, each one carrying the information for a different frequency bandpass or spatial region of the input object. The coherence coding is analogous to time multiplexing but with multiplexing time slots that are given by the coherence time of the illumination beam. Most images are static during times much longer than this coherence time, and thus the increase of resolution in our system is obtained without any noticeable c…
Exon deletions of the PAH gene in Italian hyperphenylalaninemics
2010
A consistent finding of many studies describing the spectrum of mutant phenylalanine hydroxylase alleles underlying hyperphenylalaninemia is the impossibility of achieving a 100% mutation ascertainment rate using conventional gene-scanning methods such as Denaturing Gradient Gel Electrophoresis (DGGE), Denaturing High Performance Liquid Chromatography (DHPLC), direct sequencing. In recent years, it has been shown that a significant proportion of these undetermined alleles consist of large deletions overlapping one or more exons. These deletions have been difficult to detect in compound heterozygotes by gene-scanning methods due to the masking effect by the non-deleted allele. To date no sys…
An Improved Detection Technique for Cyclic-Prefixed OFDM
2010
A novel Orthogonal Frequency Division Multiplexing detection technique compatible to standard (e.g. Wireless LAN) transmitters is proposed. It features enhanced error-rate performance with flexible computational complexity and robustness to imperfect channel estimation. It is based on exploitation of the redundancy available in the cyclic prefix after cancellation of interference from the preceding block. In order to show the effectiveness of our proposal, an analysis of computational complexity and a number of comparisons to the standard per-subcarrier receiver and a previously existing method in terms of error rates are reported.
Resource allocation for clustered network MIMO OFDMA systems
2012
In this article, we address the resource allocation problem for the downlink of a large network multiple input multiple output orthogonal frequency division multiplexing system with 3-sector base stations. The system is statically divided into a number of disjoint clusters of sectors. A two-step resource allocation scheme is proposed involving the inter-cluster and the intra-cluster levels. As a first step or inter-cluster level, two cooperative frequency reuse approaches are designed to mitigate the inter-cluster interference. A user partition method is proposed to divide the users of each cluster into cluster-edge and cluster-center users. To balance the cell-edge and the cell-average per…
Energy-Efficient and Secure Resource Allocation for Multiple-Antenna NOMA with Wireless Power Transfer
2018
Non-orthogonal multiple access (NOMA) is considered as one of the promising techniques for providing high data rates in the fifth generation mobile communication. By applying successive interference cancellation schemes and superposition coding at the NOMA receiver, multiple users can be multiplexed on the same subchannel. In this paper, we investigate resource allocation algorithm design for an OFDM-based NOMA system empowered by wireless power transfer. In the considered system, users who need to transmit data can only be powered by the wireless power transfer. With the consideration of an existing eavesdropper, the objective is to obtain secure and energy efficient transmission among mul…
Continuous Monitoring of Parasitic Elements in Boost Converter Circuit
2021
The given paper explains the necessity of condition monitoring for DC/DC boost converter circuit. Further, an analytical model of circuit parasitic estimation is presented based on measured quantities in the circuit. The implementation of continuous estimation of circuit parasitic elements is analytically explained and verified by simulations and experimental results. Obtained results are acceptable for condition monitoring.
Wireless ECG and cardiac monitoring systems: State of the art, available commercial devices and useful electronic components
2021
Abstract Wireless ElectroCardioGram (ECG) systems are employed in manifold application fields: tele-monitoring, sport applications, support to ageing people at home, fetal ECG, wearable devices and ambulatory monitoring. The presence of cables often hinders user’s free movements, alongside clinicians’ routine operations. Therefore, wireless ECG systems are desirable. This paper aims at reviewing the solutions described in the literature, besides commercially available devices and electronic components useful to setup laboratory prototypes. Several systems have been developed, different in terms of the adopted technology; when approaching the development of a wireless ECG system, some import…