Search results for "NAD"

showing 10 items of 2033 documents

Regulation of reactive oxygen species production by a 14-3-3 protein in elicited tobacco cells.

2007

International audience; The regulation of the system responsible for the production of reactive oxygen species (ROS) during plant–microorganism interaction is still largely unknown. The protein NtrbohD has been recently demonstrated as the plasma membrane oxidase responsible for ROS production in elicited tobacco cells. Here, its C-terminus part was used as a bait in a two-hybrid screen in order to identify putative regulators of this system. This led to the isolation of a cDNA coding for a member of the 14-3-3 protein family. The corresponding transcript was induced after infiltration of tobacco leaves with the fungal elicitor cryptogein. Tobacco cells transformed with an antisense constru…

0106 biological sciencesSIGNALLINGDNA ComplementaryProtein familyPhysiologyMolecular Sequence DataContext (language use)Plant ScienceBiology01 natural sciences03 medical and health sciencesTwo-Hybrid System TechniquesTobaccoNADPH OXIDASEAmino Acid Sequence14-3-3 protein030304 developmental biologychemistry.chemical_classification[SDV.EE]Life Sciences [q-bio]/Ecology environment0303 health sciencesReactive oxygen speciesOxidase testCRYPTOGEINNADPH oxidaseSequence Homology Amino AcidElicitorchemistryBiochemistry14-3-3 ProteinsNAD(P)H oxidasebiology.proteinReactive Oxygen Species010606 plant biology & botanyPlant, cellenvironment
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Seasonal variability of diet and trophic level of the gelatinous predator Pelagia noctiluca (Scyphozoa)

2018

13 pages, 5 figures, 2 tables, supplementary information https://doi.org/10.1038/s41598-018-30474-x

0106 biological sciencesSettore BIO/07 - EcologiaJellyfishFood ChainScyphozoaZoologylcsh:Medicine010603 evolutionary biology01 natural sciencesArticleZooplanktonPredationbiology.animalMediterranean SeaAnimals14. Life underwaterBites and StingsGonadslcsh:SciencePredatorTrophic levelCarbon IsotopesDetritusMultidisciplinarybiologyNitrogen Isotopes010604 marine biology & hydrobiologyFatty Acidslcsh:RScyphozoa15. Life on landPlanktonbiology.organism_classificationPelagia noctilucajellyfish stomach content stable isotope fatty acid food habit outbreak-forming speciesGastrointestinal Contents13. Climate actionPredatory Behaviorlcsh:QSeasonsBiomarkersScientific Reports
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Effects of starvation on survival, cannibalism, body mass, and intestinal protozoan profile in the subterranean termite Reticulitermes lucifugus

2019

Scarcity or inadequate nutrition can affect biological and behavioural aspects of subterranean termites and their intestinal protozoan profile. The aim of this study was to investigate changes in survival, cannibalism, body mass, and protist community structure of Reticulitermes lucifugus Rossi subspecies “Sicily” following starvation to provide basic knowledge for the development of more specific studies on a possible survival strategy under stressful conditions. In nature, this termite consumes many food sources and its feeding activity is continuous during the year. In a 35-day laboratory experiment, groups of 50 termites (worker/soldier ratio 49:1) were subjected to two diets, starvatio…

0106 biological sciencesStarvationSocial insectProtistInadequate nutritionCannibalismBehaviour changeZoologySurvivorshipBiologyIntestinal protozoan010603 evolutionary biology01 natural sciences010602 entomologySettore AGR/11 - Entomologia Generale E ApplicataBasic knowledgeInsect ScienceSurvivorship curveSurvival strategymedicineReticulitermes lucifugusmedicine.symptomLaboratory experimentEcology Evolution Behavior and SystematicsInsectes Sociaux
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The plasma membrane oxidase NtrbohD is responsible for AOS production in elicited tobacco cells

2002

Summary A cDNA encoding a protein, NtrbohD, located on the plasma membrane and homologue to the flavocytochrome of the neutrophil NADPH oxidase, was cloned in tobacco. The corresponding mRNA was accumulated when tobacco leaves and cells were treated with the fungal elicitor cryptogein. After elicitation with cryptogein, tobacco cells transformed with antisense constructs of NtrbohD showed the same extracellular alkalinization as the control, but no longer produced active oxygen species (AOS). This work represents the first demonstration of the function of a homologue of gp91–phox in AOS production in elicited tobacco cells.

0106 biological sciencesTime FactorsNicotiana tabacumMolecular Sequence DataPlant ScienceBiologyGenes Plant01 natural sciencesFungal Proteins[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics03 medical and health sciences[SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants geneticsComplementary DNATobaccoGene expressionGeneticsExtracellularAOSAmino Acid SequenceRNA MessengerCells CulturedComputingMilieux_MISCELLANEOUS030304 developmental biology0303 health sciencesOxidase testNADPH oxidaseGene Expression ProfilingAlgal ProteinsCell MembraneHydrogen PeroxideCell BiologyHydrogen-Ion ConcentrationPlants Genetically Modifiedbiology.organism_classification3. Good healthElicitorCell biologyPlant LeavesProtein TransportBiochemistryCell culturebiology.proteinOxidoreductasesReactive Oxygen Species010606 plant biology & botany
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Active vanadate-sensitive H+ translocation in corn roots membrane vesicles and proteoliposomes

1988

Abstract A member fraction from corn roots which contains a vanadate-sensitive ATPase activity has been prepared. The specific activity at 38°C is between 3 and mol 12 μmol · min −1 · mg −1 , depending on the age of roots. Addition of ATP promotes a very rapid quenching of the fluorescence of 9-amino-6-chloro-3-methoxy-acridin (ACMA). Proton pumping exhibits a delayed sensitivity to vanadate but is strongly and instantaneously inhibited by the new inhibitor SW 26. Both proton pumping, measured by the initial quenching rate, and ATP hydrolysis show maximum activities at ATP concentrations in the millimolar range, but the apparent K m -value for hydrolysis is higher than that observed for pro…

0106 biological sciencesTrisMolar concentrationATPase[SDV]Life Sciences [q-bio]Plant Science01 natural sciences03 medical and health scienceschemistry.chemical_compoundHydrolysisATP hydrolysisProton transportGeneticsVanadateComputingMilieux_MISCELLANEOUS030304 developmental biology0303 health sciencesQuenching (fluorescence)biologyGeneral Medicine[SDV] Life Sciences [q-bio]chemistryBiochemistrybiology.proteinBiophysicsAgronomy and Crop Science010606 plant biology & botany
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IPSIM-Cirsium, a Qualitative Expert-Based Model to Predict Infestations of Cirsium arvense

2021

Throughout Europe, Cirsium arvense is the most problematic perennial weed in arable crops, whether managed under organic or conventional agriculture. Non-chemical control methods are limited with partial efficacy. Knowledge is missing on their effect across a wide gradient of cropping systems and pedoclimates. To achieve effective Cirsium arvense management ensuring crop productivity while limiting the reliance of cropping systems on herbicide, expert-based models are needed to gather knowledge on the effect of individual levers and their interactions in order to (i) design and assess finely tuned combinations of farming practices in different pedoclimates and (ii) support decisions for Cir…

0106 biological sciencescropping practicesAgricultural engineeringlcsh:Plant culture01 natural sciencessoillcsh:AgricultureCirsiumlcsh:SB1-1110Cirsium arvenseclimateAgroecologyIPSIM-CirsiumMathematics2. Zero hungerbiologyCirsium arvensebusiness.industrylcsh:SGeneral MedicineCanada thistle04 agricultural and veterinary sciences15. Life on landWeed controlbiology.organism_classificationinjury profile SIMulatorintegrated weed managementAgriculture[SDE]Environmental Sciences040103 agronomy & agriculture0401 agriculture forestry and fisheriesqualitative modelingArable landbusinessWeedCroppingqualitative modelling010606 plant biology & botanyFrontiers in Agronomy
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Resource availability and competition shape the evolution of survival and growth ability in a bacterial community

2013

Resource availability is one of the main factors determining the ecological dynamics of populations or species. Fluctuations in resource availability can increase or decrease the intensity of resource competition. Resource availability and competition can also cause evolutionary changes in life-history traits. We studied how community structure and resource fluctuations affect the evolution of fitness related traits using a two-species bacterial model system. Replicated populations of Serratia marcescens (copiotroph) and Novosophingobium capsulatum (oligotroph) were reared alone or together in environments with intergenerational, pulsed resource renewal. The comparison of ancestral and evol…

0106 biological sciencesecological nichesBACTERIAL BIOFILMSResource (biology)media_common.quotation_subjectScienceAdaptation BiologicalBiologyco-evolutionEnvironment010603 evolutionary biology01 natural sciencesCompetition (biology)Bacterial evolution03 medical and health sciencesSpecies SpecificityAdaptive radiationCopiotrophSerratia marcescens030304 developmental biologymedia_commonEcological niche0303 health sciencesMultidisciplinaryEcologyMicrobiotaQCommunity structureRInterspecific competitionbiology.organism_classificationBiological EvolutionSurvival AnalysisSphingomonadaceaeSerratia marcescens1181 Ecology evolutionary biologyLinear ModelsMedicineMicrobial Interactionsta1181competitionResearch ArticlePLOS ONE
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The Spatial Dimension in Population Fluctuations

1997

Theoretical research into the dynamics of coupled populations has suggested a rich ensemble of spatial structures that are created and maintained either by external disturbances or self-reinforcing interactions among the populations. Long-term data of the Canadian lynx from eight Canadian provinces display large-scale spatial synchrony in population fluctuations. The synchronous dynamics are not time-invariant, however, as pairs of populations that are initially in step may drift out of phase and back into phase. These observations are in agreement with predictions of a spatially-linked population model and support contemporary population ecology theory.

0106 biological scienceseducation.field_of_studyMultidisciplinarybiology010604 marine biology & hydrobiologyPopulationCanadian lynxPopulation ecologybiology.organism_classification010603 evolutionary biology01 natural sciencesOut of phaseGeographyDimension (vector space)Population modelClimatologySynchronismeducationSpatial organizationScience
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Seasonal changes in European whitefish muscle and invertebrate prey fatty acid composition in a subarctic lake

2019

Ambient light and temperature show extreme seasonal variation in subarctic lakes due to the midnight sun period in summer and cold polar night period in winter. These changes have clear impacts on fish feeding and reproduction cycles, potentially affecting the fatty acid (FA) composition of muscle. Despite extensive research into fish FA over recent decades, we know little about intra‐annual changes of fish FA profile and content. We studied intra‐annual changes in the FA profile (mol%) and content (mg g‐1 dry weight) of sexually mature European whitefish (Coregonus lavaretus) muscle in a large and deep subarctic lake located in northern Fennoscandia. We collected fish, zooplankton, and ben…

0106 biological sciencesrasvahapotspawningAquatic ScienceBiology010603 evolutionary biology01 natural sciencesZooplanktontalvehtiminenravintochemistry.chemical_compoundAnimal scienceDry weighthighly unsaturated fatty acidsdiet shiftvaihtelu14. Life underwaterVDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920vuodenajatPolar night010604 marine biology & hydrobiologyn‐3/n‐6Subarctic climateEicosapentaenoic acidkutuGonadosomatic Indexchemistrysiika13. Climate actionDocosahexaenoic acidannualwinter ecologyStearidonic acidFreshwater Biology
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Migration strategies of brown trout (Salmo trutta) in a subarctic river system as revealed by stable isotope analysis

2018

We estimated the proportions of anadromous and freshwater‐resident brown trout (Salmo trutta) in different parts of the subarctic River Näätämöjoki/Neidenelva system (Finland and Norway) using carbon, nitrogen and hydrogen stable isotope analyses of archived scales as identifiers of migration strategy. Our results showed that carbon stable isotope values were the best predictor of migration strategy. Most individuals fell into two clearly distinct groups representing anadromous (47%) or freshwater‐resident (42%) individuals, but some fish had intermediate carbon values suggesting repeated movement between freshwater and the sea. The proportion of anadromous individuals decreased steadily wi…

0106 biological sciencesvaelluskalatpartial migrationEcologyEcology010604 marine biology & hydrobiologysalmonidAquatic ScienceBiologybiology.organism_classification010603 evolutionary biology01 natural sciencesSubarctic climateLife history theoryanadromyBrown troutmigraatio (biologia)taimenlife-history strategiesta1181SalmoresidencyEcology Evolution Behavior and SystematicsIsotope analysisEcology of Freshwater Fish
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