Search results for "NEXT"
showing 10 items of 178 documents
Next-Generation Sequencing in Clinical Practice
2019
Abstract During the past few decades, Sanger sequencing represented the “gold standard” technique. In order to better define the mutational status of several genes at the same time, next-generation sequencing methodologies have been introduced in the molecular laboratory workflow. In the era of personalized medicine, this technological improvement plays a key role in the comprehensive molecular characterization of cancer patients in order to get a “tile” target therapy. For different cancer patients, different target therapies are available and different genes serve as clinical it relevant biomarkers. This chapter reviews the principal features of these novel technologies and their applicat…
The child with overgrowth between clinical variability and genetic heterogeneity
2020
Either in the newborn or in the child overgrowth can be generalized or localized if it is limited to one or more body regions. When overgrowth depends on a metabolic imbalance, or it is constitutional, the excessive growth can be the only clinical sign. In most cases genomic or epigenetic alterations, which affect factors involved in cell proliferation and/or regulation of gene expression (observed also in tumours), are related to overgrowth syndromes, in which excess growth may be associated with dysmorphic features, neuromotor/intellectual disabilities and behavioural disorders. These rare conditions are characterized by clinical and molecular overlap. The paper describes the cases of thr…
Metagenomic, microbiological, chemical and sensory profiling of Caciocavallo Podolico Lucano cheese
2023
In this study, Caciocavallo Podolico Lucano (CPL) cheese was deeply characterized for its bacterial community, chemical composition and sensory aspects. The entire cheese making process (from milk collection to ripened cheese) was performed by strictly applying the traditional protocol for CPL production in four dairy factories (A–D) representative of the production area. The vat made of wood represents the main transformation tool for CPL cheese production and the biofilms hosted onto the internal surfaces of all vats analyzed in this study were dominated by lactic acid bacteria. Total mesophilic microorganisms present in bulk milk (4.7–5.0 log CFU/ml) increased consistently after contact …
Lightweight BWT Construction for Very Large String Collections
2011
A modern DNA sequencing machine can generate a billion or more sequence fragments in a matter of days. The many uses of the BWT in compression and indexing are well known, but the computational demands of creating the BWT of datasets this large have prevented its applications from being widely explored in this context. We address this obstacle by presenting two algorithms capable of computing the BWT of very large string collections. The algorithms are lightweight in that the first needs O(m log m) bits of memory to process m strings and the memory requirements of the second are constant with respect to m. We evaluate our algorithms on collections of up to 1 billion strings and compare thei…
Lightweight algorithms for constructing and inverting the BWT of string collections
2013
Recent progress in the field of \{DNA\} sequencing motivates us to consider the problem of computing the Burrows‚ÄìWheeler transform (BWT) of a collection of strings. A human genome sequencing experiment might yield a billion or more sequences, each 100 characters in length. Such a dataset can now be generated in just a few days on a single sequencing machine. Many algorithms and data structures for compression and indexing of text have the \{BWT\} at their heart, and it would be of great interest to explore their applications to sequence collections such as these. However, computing the \{BWT\} for 100 billion characters or more of data remains a computational challenge. In this work we ad…
Media Delivery to Remote Renderers Controlled by the Mobile Phone
2009
In today's content delivery solutions, service delivery and control are still tightly coupled, a service typically being delivered to the same device that controls the session. We present a solution that was designed with the goal to decouple service control and delivery. Using our approach, multimedia streaming services can be delivered to off-the-shelf DLNA devices in visited networks. The service provider receives information about the remote media player and access environment via a mobile phone. Proximity technologies (e.g. barcodes, NFC) of the control device are used for the exchange of required credentials. This paper describes a typical scenario and our prototype implementation.
A global service level guarantee in NGN networks
2010
In this paper, we propose an end-to-end service level negotiation protocol which enables a global service offer covering quality of service (QoS) and security while taking into account user's mobility in this offer. This protocol provides all the domains involved in the transport of a various offered services with a negotiation capability to achieve an agreement on a service level. This paper shows also results of measurements evaluating the performances of the considered negotiation protocol and security impact on QoS.
UWSIO: Towards automatic orchestration for the deployment of 5G monitoring services from bare metal
2018
The next generation mobile networks 5G are currently being intensively developed and standardized globally, with commercial prototyping 5G connections already emerging. At the 5G system level, one of the Key Performance Indicators (KPIs) defining 5G is substantially reduced service creation time for 5G network operators and/or service providers to increase the system efficiency and thus reduce operational costs. In this work, we focus on realize this challenging KPI in terms of speedy creation of monitoring services for 5G operators from scratch (no operating system pre-installed). A new 5G infrastructure orchestrator UWSIO is proposed to achieve fully automated deployment of 5G monitoring …
An Integrated Multi-service Software Simulation Platform: SIMPSONS Architecture
2005
This paper describes SIMPSONS (SIp and MPls Simulations On NS-2), which is a software platform for simulating multiple network scenarios, such as Telephony Over IP (TOIP), and multi-services networks extending the functionalities of Network Simulator NS-2. The innovative aspects of SIMPSONS is the complete integration of control protocols and traffic engineering mechanisms inside the same simulation tool. In fact, next generation networks must meet basically two fundamental requirements: support of Quality of Service (QoS) and Traffic Engineering (TE) functionalities. SIMPSONS is able to simulate DiffServ, MPLS, OSPF and SIP and their interaction in everything TOIP scenario. So with this po…
Transport service for session initiation protocol in SIP-T scenarios
2005
The traditional telephone service has undergone deep changes in the years concerning the techniques of commutation and signaling. Today, public switched telecom networks (PSTNs) and private integrated services networks (PINSs) are strongly directed to adopt IP-based backbone. If it is possible to transfer the voice on packet switching network, in such reliable way, using voice over IP (VoIP) techniques, there is not still a valid alternative to the signaling systems, reliable and unfailing, like signaling system n.7 (SST). Session initiation protocol (SIP) seems to be the best candidate. SIP is an application layer signaling protocol and the problem remains still opened for what it concerns…