Search results for "NGS"

showing 10 items of 6306 documents

Early experiences from one of the first treatment programs for chronic hepatitis B in sub-Saharan Africa

2017

Treatment for chronic hepatitis B (CHB) is virtually absent in sub-Saharan Africa. Here we present early experiences from a pilot program for treatment of CHB in Ethiopia. Adults (≥18 years) with CHB were included in a cohort study at St. Paul’s Hospital Millennium Medical College, Addis Ababa, from February 2015. The baseline assessment included liver function tests, viral markers and transient elastography (Fibroscan 402, Echosense, France). Logistic regression models were used to identify predictors of fibrosis. Tenofovir disoproxil fumarate (TDF) was initiated based on the European Association for the Study of the Liver (EASL) criteria, with some modifications. The initial 300 patients …

AdultLiver CirrhosisMaleHepatitis B virusmedicine.medical_specialtyCirrhosisPilot ProjectsAntiviral therapyAntiviral Agentslcsh:Infectious and parasitic diseasesCohort StudiesYoung Adult03 medical and health sciencesHepatitis B Chronic0302 clinical medicinePregnancyInterquartile rangeInternal medicinemedicineHumanslcsh:RC109-216Hepatitis B e Antigens030212 general & internal medicinePregnancy Complications InfectiousTenofovirAIDS-Related Opportunistic Infectionsmedicine.diagnostic_testCoinfectionbusiness.industryAlanine TransaminaseMiddle AgedViral LoadHepatitis Bmedicine.diseaseResource-limited settingsInfectious DiseasesHBeAgAfricaImmunologyFemale030211 gastroenterology & hepatologyEthiopiaTransient elastographyLiver function testsbusinessViral loadBiomarkersResearch ArticleCohort study
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Treatment of chronic hepatitis B in sub-Saharan Africa: 1-year results of a pilot program in Ethiopia

2018

Background The World Health Organization has set an ambitious goal of eliminating viral hepatitis as a major public health threat by 2030. However, in sub-Saharan Africa, antiviral treatment of chronic hepatitis B (CHB) is virtually unavailable. Herein, we present the 1-year results of a pilot CHB treatment program in Ethiopia. Methods At a public hospital in Addis Ababa, CHB patients were treated with tenofovir disoproxil fumarate based on simplified eligibility criteria. Baseline assessment included liver function tests, viral markers, and transient elastography (Fibroscan). Changes in laboratory markers were analyzed using Wilcoxon signed-rank tests. Adherence to therapy was measured by …

AdultLiver CirrhosisMalemedicine.medical_specialtyAdolescentEpidemiologylcsh:MedicinePilot ProjectsAntiviral therapymedicine.disease_causeAntiviral AgentsYoung Adult03 medical and health sciencesHepatitis B Chronic0302 clinical medicineChronic hepatitisInternal medicineEpidemiologymedicineHumansPilot program030212 general & internal medicineViral hepatitisTenofovirHepatitis B virusmedicine.diagnostic_testbusiness.industryPublic healthlcsh:RGeneral MedicineMiddle AgedViral Loadmedicine.diseaseResource-limited settingsTreatment Adherence and ComplianceTreatment OutcomeFemale030211 gastroenterology & hepatologyEthiopiaTransient elastographybusinessLiver function testsViral hepatitisBiomarkersFollow-Up StudiesResearch ArticleBMC Medicine
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Predictors of mortality in patients under treatment for chronic hepatitis B in Ethiopia: a prospective cohort study

2019

Abstract Background Antiviral treatment for chronic hepatitis B (CHB) is largely unavailable in sub-Saharan Africa; hence, little is known about the prognosis after initiating treatment in African CHB patients. In this study we aimed to assess predictors of mortality in one of the largest CHB cohorts in sub-Saharan Africa. Methods Two-hundred-and-seventy-six CHB patients who started treatment with tenofovir disoproxil fumarate at a public hospital in Ethiopia between March 18, 2015, and August 1, 2017, were included in this analysis. Patients were followed up until October 1, 2017, and deaths were ascertained through hospital records and telephone interview with relatives. Decompensated cir…

AdultLiver CirrhosisMalemedicine.medical_specialtyTime FactorsSurvivalAdolescentEpidemiologyPhysical examinationAntiviral therapyAntiviral AgentsBody Mass Index03 medical and health sciencesYoung Adult0302 clinical medicineHepatitis B ChronicInterquartile rangeRisk FactorsInternal medicineEpidemiologyMedicineHumansProspective Studieslcsh:RC799-869Prospective cohort studyTenofovirProportional Hazards Modelsmedicine.diagnostic_testbusiness.industryHazard ratioGastroenterologyAge FactorsGeneral MedicineHepatitis BHepatologyMiddle Agedmedicine.diseaseHepatitis BResource-limited settingsSurvival RateVDP::Medisinske Fag: 700::Helsefag: 800030220 oncology & carcinogenesislcsh:Diseases of the digestive system. Gastroenterology030211 gastroenterology & hepatologyFemaleEthiopiabusinessBody mass indexResearch Article
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Potential clinical value of quantitative fluorine-18-fluorodeoxyglucose-PET/computed tomography using a graph-based method analysis in evaluation of …

2019

Objectives To identify the clinical relevance of incidentally detected lesions (IDLs) in the gastrointestinal tract (GIT) with 18F-FDG PET/CT and to assess the potential benefit of using semiquantitative PET measures to discern malignant from benign lesions. Methods Forty-one patients who underwent F-FDG PET/CT scans during the oncologic follow-up, revealing abnormal incidental 18F-FDG accumulations in the GIT were included in this retrospective analysis. Incidental PET/CT findings were correlated with endoscopic and histological findings. Semiquantitative PET values (SUVmax, SUVmean, SULpeak, and TLG) were evaluated by using a new graph-based method. Two sample t-test analysis has been per…

AdultMale-Endoscopy Gastrointestinal030218 nuclear medicine & medical imagingCorrelation03 medical and health sciences0302 clinical medicineFluorodeoxyglucose F18Positron Emission Tomography Computed TomographyBiopsymedicineImage Processing Computer-AssistedHumansRadiology Nuclear Medicine and imagingClinical significanceGastrointestinal NeoplasmsRetrospective StudiesGastrointestinal tractIncidental FindingsFluorine-18-fluorodeoxyglucosemedicine.diagnostic_testbusiness.industryRetrospective cohort studyGeneral Medicinemedicine.diseaseEndoscopyTumor BurdenDysplasia030220 oncology & carcinogenesisFemalebusinessNuclear medicineGlycolysisNuclear medicine communications
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Influence of the structural components of artificial turf systems on impact attenuation in amateur football players

2019

AbstractThe purpose of this research was to evaluate the influence of the structural components of different 3rd generation artificial turf football field systems on the biomechanical response of impact attenuation in amateur football players. A total of 12 amateur football players (24.3 ± 3.7 years, 73.5 ± 5.5 kg, 178.3 ± 4.1 cm and 13.7 ± 4.3 years of sport experience) were evaluated on three third generation artificial turf systems (ATS) with different structural components. ATS were composed of asphalt sub-base and 45 mm of fibre height with (ATS1) and without (ATS2) elastic layer or compacted granular sub-base, 60 mm of fibre height without elastic layer (ATS3). Two triaxial accelerome…

AdultMale0301 basic medicineAccelerationPoison controlSTRIDElcsh:MedicineFútbolArticleLower energyRunningYoung Adult03 medical and health sciences0302 clinical medicineFloors and FloorcoveringsSoccerArtificial turfHumanslcsh:ScienceMathematicsOrthodonticsEsportsFootball playersMultidisciplinaryAttenuationlcsh:RDeporteBone quality and biomechanicsBiomechanical Phenomena030104 developmental biologyRisk factorsAthletesFootball fieldSuperficies deportivas sintéticaslcsh:QEducació físicaAmateur030217 neurology & neurosurgeryInstalación deportiva
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Identification of a novel LMF1 nonsense mutation responsible for severe hypertriglyceridemia by targeted next-generation sequencing

2016

Background Severe hypertriglyceridemia (HTG) may result from mutations in genes affecting the intravascular lipolysis of triglyceride (TG)-rich lipoproteins. Objective The aim of this study was to develop a targeted next-generation sequencing panel for the molecular diagnosis of disorders characterized by severe HTG. Methods We developed a targeted customized panel for next-generation sequencing Ion Torrent Personal Genome Machine to capture the coding exons and intron/exon boundaries of 18 genes affecting the main pathways of TG synthesis and metabolism. We sequenced 11 samples of patients with severe HTG (TG>885 mg/dL–10 mmol/L): 4 positive controls in whom pathogenic mutations had pre…

AdultMale0301 basic medicineCandidate geneEndocrinology Diabetes and MetabolismDNA Mutational AnalysisNonsense mutationPanel-based NGS sequencing030204 cardiovascular system & hematologyBiologymedicine.disease_causeDNA sequencing03 medical and health sciencessymbols.namesakeExon0302 clinical medicineNutrition and DieteticInternal MedicinemedicineHumansGeneHypertriglyceridemiaSanger sequencingGeneticsMutationNutrition and DieteticsLMF1 geneNonsense mutationHigh-Throughput Nucleotide SequencingInfantMembrane ProteinsIon semiconductor sequencingMiddle AgedIon torrent PGM sequencingPhenotype030104 developmental biologyChild PreschoolsymbolsFemaleCardiology and Cardiovascular MedicineJournal of Clinical Lipidology
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Dermatitis due to Mesostigmatic mites (Dermanyssus gallinae, Ornithonyssus [O.] bacoti, O. bursa, O. sylviarum ) in residential settings

2018

No abstract available (letter to the Editor)

AdultMale0301 basic medicineMite InfestationsVeterinary medicineDermanyssus gallinae040301 veterinary sciencesDermatitisDermatologyBiologymedicine.disease_causeDermatiti0403 veterinary scienceResidential settings.03 medical and health sciencesInfestationSettore MED/35 - Malattie Cutanee E VenereemedicineAnimalsHumansOrnithonyssusRetrospective StudiesMite InfestationsOutbreak04 agricultural and veterinary sciencesMiddle Agedbiology.organism_classification030104 developmental biologyMesostigmatic miteFemaleJDDG: Journal der Deutschen Dermatologischen Gesellschaft
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Impact of a 4-Week Intensified Endurance Training Intervention on Markers of Relative Energy Deficiency in Sport (RED-S) and Performance Among Well-T…

2020

Cyclists often apply block periodization to high training volumes in meso- and macrocycles to optimize training adaptation and to prepare for competition. Body mass influences performance in many sports, including endurance disciplines, and conditions related to the syndrome Relative Energy Deficiency in Sports (RED-S) such as metabolic adaptations and premature osteoporosis have also been reported in male cyclists. This study aimed to determine how a 4-week mesocycle of intensified endurance training designed to increase performance, would affect markers of RED-S in well-trained male cyclists. Twenty-two participants (age: 33.5 ± 6.6 years, height: 181.4 ± 5.2 cm, weight: 76.5 ± 7.4 kg, pe…

AdultMale0301 basic medicineVDP::Medisinske Fag: 700::Idrettsmedisinske fag: 850::Treningslære: 851Endocrinology Diabetes and Metabolismmale cyclistsOsteoporosisenergy availability030209 endocrinology & metabolismAthletic PerformanceHigh-Intensity Interval Traininglcsh:Diseases of the endocrine glands. Clinical endocrinologyhormonal responseInterval training03 medical and health sciencesEndocrinology0302 clinical medicineAnimal scienceEndurance trainingHumansRelative Energy Deficiency in SportMedicineProspective Studiesresting metabolic rateTestosteroneOriginal Researchlcsh:RC648-665Triiodothyroninebusiness.industryendurance athletesVO2 maxVDP::Medisinske Fag: 700::Idrettsmedisinske fag: 850medicine.diseaseEndurance Training030104 developmental biologytestosteroneBasal metabolic ratetraining interventionBasal MetabolismbusinessBiomarkersRelative energyFrontiers in Endocrinology
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A case of bowel schistosomiasis not adhering to endoscopic findings

2005

Schistosomiasis is a chronic worm infection caused by a species of trematodes, the Schistosomes. We may distinguish a urinary form from Schistosomes haematobium and an intestinal-hepatosplenic form mainly from Schistosomes mansoni characterized by nausea, meteorism, abdominal pain, bloody diarrhea, rectal tenesmus, and hepatosplenomegaly. These infections represent a major health issue in Africa, Asia, and South America, but recently S mansoni has increased its prevalence in other continents, such as Europe countries and North America, due to international travelers and immigrants, with several diagnostic and prevention problems. We report a case of a 24-year-old patient without HIV infecti…

AdultMaleAbdominal painmedicine.medical_specialtySettore MED/09 - Medicina InternaHepatosplenomegalyColonoscopyCase ReportSchistosomiasisGastroenterologyPraziquantelFeceschemistry.chemical_compoundMesalazineIntestinal mucosaInternal medicineparasitic diseasesmedicineAnimalsHumansIntestinal MucosaParasite Egg CountSchistosomaAnthelminticsbiologymedicine.diagnostic_testGastroenterologybowel schistosomiasis; endoscopic findingsGeneral Medicinebiology.organism_classificationmedicine.diseaseRectal tenesmusSchistosomiasis mansonichemistryImmunologySchistosomamedicine.symptomWorld Journal of Gastroenterology
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Childhood Physical Activity and Adulthood Earnings

2016

Purpose: This study examined the associations between childhood physical activity level and adulthood earnings. Methods: The data were drawn from the ongoing longitudinal Young Finns Study, which was combined with register-based Finnish Longitudinal Employer–Employee Data and registerbased parents_ background information from the Longitudinal Population Census of Statistics Finland. The study consisted of children who were 9 yr (n = 1257, 52% boys), 12 yr (n = 1662, 51% boys), and 15 yr (n = 1969, 49% boys) of age at the time when physical activity was measured. The children were followed until 2010, when they were between 33 and 45 yr old. Leisure-time physical activity in childhood was se…

AdultMaleAdolescentPhysical activityphysical activityPhysical Therapy Sports Therapy and RehabilitationDevelopmental psychology03 medical and health sciences0302 clinical medicinechildren0502 economics and businessadultsHumansOrthopedics and Sports MedicineLongitudinal Studies050207 economicsChildta315Exerciseta512Finlandaikuisetta511Earnings05 social scienceshealth030229 sport sciencesPhysical activity levelIncomeFemalelabor marketPsychologyterveysearningsMedicine and Science in Sports and Exercise
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