Search results for "NIH"

showing 10 items of 1190 documents

The transcriptional programme of contact-inhibition.

2010

Proliferation of non-transformed cells is regulated by cell-cell contacts, which are referred to as contact-inhibition. Vice versa, transformed cells are characterised by a loss of contact-inhibition. Despite its generally accepted importance for cell-cycle control, little is known about the intracellular signalling pathways involved in contact-inhibition. Unravelling the molecular mechanisms of contact-inhibition and its loss during tumourigenesis will be an important step towards the identification of novel target genes in tumour diagnosis and treatment. To better understand the underlying molecular mechanisms we identified the transcriptional programme of contact-inhibition in NIH3T3 fib…

Blotting WesternClone (cell biology)Cell Cycle ProteinsBiologyBiochemistryMiceComplementary DNATranscriptional regulationAnimalsMolecular BiologyGeneRegulator geneOligonucleotide Array Sequence AnalysisContact InhibitionReverse Transcriptase Polymerase Chain ReactionGene Expression ProfilingCell CycleContact inhibitionCell BiologyFibroblastsFlow CytometryMolecular biologyGene expression profilingNIH 3T3 CellsDNA microarraySignal TransductionJournal of cellular biochemistry
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Evidence for long-lived, optically generated quenchers of excitons in single-walled carbon nanotubes.

2011

The nonlinear dependence of near-infrared photoluminescence (PL) emission on excitation intensity has been measured for individual nanotubes representing six different (n,m) species. Significant deviations from linearity are observed for intensities as low as ~100 W/cm(2), and an approximate inverse correlation is found between nonlinearity and PL action cross section (brightness). A model in which all PL nonlinearity arises from exciton-exciton annihilation is insufficient to account for the experimental data using realistic parameters. It is proposed that additional nonlinear quenching arises from photoinduced quenching states or species with longer lifetimes than emissive excitons. Evide…

BrightnessPhotoluminescenceLightExcitonBioengineeringCarbon nanotubelaw.inventionAbsorptionCondensed Matter::Materials SciencelawMetastabilityScattering RadiationGeneral Materials ScienceComputer SimulationParticle SizeQuenching (fluorescence)AnnihilationChemistryNanotubes CarbonMechanical EngineeringGeneral ChemistryCondensed Matter PhysicsRefractometryModels ChemicalAtomic physicsExcitationNano letters
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The receptor NLRP3 is a transcriptional regulator of TH2 differentiation.

2015

The receptor NLRP3 is involved in the formation of the NLRP3 inflammasome that activates caspase-1 and mediates the release of interleukin 1β (IL-1β) and IL-18. Whether NLRP3 can shape immunological function independently of inflammasomes is unclear. We found that NLRP3 expression in CD4(+) T cells specifically supported a T helper type 2 (TH2) transcriptional program in a cell-intrinsic manner. NLRP3, but not the inflammasome adaptor ASC or caspase-1, positively regulated a TH2 program. In TH2 cells, NLRP3 bound the Il4 promoter and transactivated it in conjunction with the transcription factor IRF4. Nlrp3-deficient TH2 cells supported melanoma tumor growth in an IL-4-dependent manner and …

CD4-Positive T-LymphocytesInflammasomesImmunologyBlotting WesternBiologyInterleukin 21MiceTh2 CellsCell Line TumorNLR Family Pyrin Domain-Containing 3 ProteinImmunology and AllergyCytotoxic T cellAnimalsIL-2 receptorPromoter Regions GeneticInterleukin 3Oligonucleotide Array Sequence AnalysisMice KnockoutCD40integumentary systemReverse Transcriptase Polymerase Chain ReactionZAP70Gene Expression ProfilingCell DifferentiationNeoplasms ExperimentalAsthmaCell biologyGene Expression Regulation NeoplasticMice Inbred C57BLInterleukin 10Interferon Regulatory FactorsInterleukin 12biology.proteinNIH 3T3 CellsTrans-ActivatorsFemaleInterleukin-4Carrier ProteinsProtein BindingSignal TransductionNature immunology
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Measurement and interpretation of the $W$-pair cross-section in $e^+e^-$ interactions at 161 GeV

1997

In 1996 LEP ran at a centre-of-mass energy of 161~GeV, just above the threshold of W-pair production. DELPHI accumulated data corresponding to an integrated luminosity of $9.93 {\mathrm{~pb^{-1}}}$, and observed 29 events that are considered as candidates for W-pair production. From these, a cross-section for the doubly resonant $e^+e^-\to\mathrm{WW}$ process of $3.67~^{+0.97}_{-0.85} \pm 0.19{\mathrm{~pb}}$ has been measured. Within the Standard Model, this cross-section corresponds to a mass of the W-boson of ${\mathrm{80.40~\pm~0.44~(stat.)~\pm~0.09~(syst.) ~\pm 0.03~(LEP)~GeV}}/c^2$. Alternatively, if $m_{\mathrm{W}}$ is held fixed at its current value determined by other experiments, t…

COLLISIONSNuclear and High Energy PhysicsParticle physicsElectron–positron annihilation01 natural sciencesBOSON MASS; ROOT-S=1.8 TEV; COLLISIONS; COUPLINGSPartícules (Física nuclear)Standard ModelInterpretation (model theory)Nuclear physics0103 physical sciences[PHYS.HEXP]Physics [physics]/High Energy Physics - Experiment [hep-ex]010306 general physicsDetectors de radiacióDELPHIPhysicsLuminosity (scattering theory)010308 nuclear & particles physicsROOT-S=1.8 TEVCOUPLINGSLARGE ELECTRON POSITRON COLLIDERBOSON MASSCross section (geometry)PARTICLE PHYSICS; LARGE ELECTRON POSITRON COLLIDER; DELPHIPARTICLE PHYSICSHigh Energy Physics::ExperimentParticle Physics - ExperimentPhysics Letters B
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Measurement of the lifetime of tau-lepton

1996

The tau lepton lifetime is measured with the L3 detector at LEP using the complete data taken at centre-of-mass energies around the Z pole resulting in tau_tau = 293.2 +/- 2.0 (stat) +/- 1.5 (syst) fs. The comparison of this result with the muon lifetime supports lepton universality of the weak charged current at the level of six per mille. Assuming lepton universality, the value of the strong coupling constant, alpha_s is found to be alpha_s(m_tau^2) = 0.319 +/- 0.015(exp.) +/- 0.014 (theory). The tau lepton lifetime is measured with the L3 detector at LEP using the complete data taken at centre-of-mass energies around the Z pole resulting in τ τ =293.2 ± 2.0 (stat) ± 1.5 (syst) fs . The c…

COLLISIONSNuclear and High Energy PhysicsParticle physicsLUND MONTE-CARLOPAIR PRODUCTIONElectron–positron annihilationFOS: Physical sciencesElementary particleddc:500.201 natural sciences7. Clean energyResonance (particle physics)JET FRAGMENTATIONDECAYSHigh Energy Physics - ExperimentNuclear physicsParticle decayHigh Energy Physics - Experiment (hep-ex)0103 physical sciences[PHYS.HEXP]Physics [physics]/High Energy Physics - Experiment [hep-ex]SILICON MICROVERTEX DETECTORPRECISE MEASUREMENTLimit (mathematics)QCD ANALYSIS010306 general physicsL3 EXPERIMENTCoupling constantPhysicsMuonAnnihilationTEST BEAME+E-PHYSICS010308 nuclear & particles physicsALPHA(S)High Energy Physics::PhenomenologyDetectorPair productionSPECTRAL FUNCTIONSComputingMethodologies_DOCUMENTANDTEXTPROCESSINGHigh Energy Physics::ExperimentParticle Physics - ExperimentLeptonNuclear and Particle Physics Proceedings
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Search for neutral heavy leptons produced in Z decays

1997

Weak isosinglet Neutral Heavy Leptons ($\nu_m$) have been searched for using data collected by the DELPHI detector corresponding to $3.3\times 10^{6}$ hadronic~Z$^{0}$ decays at LEP1. Four separate searches have been performed, for short-lived $\nu_m$ production giving monojet or acollinear jet topologies, and for long-lived $\nu_m$ giving detectable secondary vertices or calorimeter clusters. No indication of the existence of these particles has been found, leading to an upper limit for the branching ratio $BR($Z$^0\rightarrow \nu_m \overline{\nu})$ of about $1.3\times10^{-6}$ at 95\% confidence level for $\nu_m$ masses between 3.5 and 50 GeV/$c^2$. Outside this range the limit weakens rap…

COLLISIONSParticle physicsE+E ANNIHILATIONPhysics and Astronomy (miscellaneous)Electron–positron annihilationHadronMASSJet (particle physics)HIGH-ENERGY-PHYSICS; MONOJET PRODUCTION; E+E ANNIHILATION; MONTE-CARLO; BOSON; LIMITS; MASS; LEP; PERFORMANCE; COLLISIONSHIGH-ENERGY-PHYSICS01 natural sciences7. Clean energyMONOJET PRODUCTIONNuclear physicsLIMITSMONTE-CARLO0103 physical sciences[PHYS.HEXP]Physics [physics]/High Energy Physics - Experiment [hep-ex]010306 general physicsDELPHIBosonPhysics010308 nuclear & particles physicsBranching fractionHigh Energy Physics::PhenomenologyBOSONLEPPERFORMANCELARGE ELECTRON POSITRON COLLIDERPARTICLE PHYSICS; LARGE ELECTRON POSITRON COLLIDER; DELPHILarge Electron–Positron ColliderPARTICLE PHYSICSHigh Energy Physics::ExperimentNeutrinoParticle Physics - ExperimentLepton
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Charged particle multiplicity distributions in restricted rapidity intervals in Z0 hadronic decays.

1991

The multiplicity distributions of charged particles in restricted rapidity intervals in Z0 hadronic decays measured by the DELPHI detector are presented. The data reveal a shoulder structure, best visible for intervals of intermediate size, i.e. for rapidity limits around ±1.5. The whole set of distributions including the shoulder structure is reproduced by the Lund Parton Shower model. The structure is found to be due to important contributions from 3-and 4-jet events with a hard gluon jet. A different model, based on the concept of independently produced groups of particles, "clans", fluctuating both in number per event and particle content per clan, has also been used to analyse the pres…

COLLISIONSParticle physicsE+E ANNIHILATIONPhysics and Astronomy (miscellaneous)LUND MONTE-CARLOElectron–positron annihilationHadronElementary particlePETRA ENERGIES01 natural sciences250 GEV/CNuclear physicsDEPENDENCE0103 physical sciences[PHYS.HEXP]Physics [physics]/High Energy Physics - Experiment [hep-ex]RapidityNuclear Experiment010306 general physicsParton showerEngineering (miscellaneous)LUND MONTE-CARLO; JET PRODUCTION-RATES; E+E ANNIHILATION; 250 GEV/C; PETRA ENERGIES; COLLISIONS; DEPENDENCE; FRAGMENTATION; QCD; RESONANCEPhysics010308 nuclear & particles physicsJET PRODUCTION-RATESMultiplicity (mathematics)RESONANCEQCDCharged particleGluonPhysique des particules élémentairesHigh Energy Physics::ExperimentFRAGMENTATIONParticle Physics - Experiment
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"Me halutaan ymmärtää" : vanhempien käsityksiä fysioterapeuttisesta ohjauksesta CP-lapsen botuliinihoidon yhteydessä

2014

Kemialliset ja ortopediset hoidot ovat nousseet perinteisen fysioterapian rinnalle spastisuuden hoitokeinona. Siitä huolimatta että kahden viime vuosikymmenen aikana näiden hoitojen suosio on kasvanut, ne eivät korvaa perinteistä fysioterapiaa. Kemiallisena hoitona botuliinitoksiini A -hoidon suosio on perustunut leikkaustarpeen viivyttämiseen, kivun hallintaan sekä spastisuuden helpottumisen kautta toiminnallisuuden lisääntymiseen erityisesti lievästi tai keskivaikeasti CP-vammaisilla lapsilla ja nuorilla. Botuliinihoidon tehokas hyödyntäminen edellyttää sekä fysioterapian että perheen fysioterapeuttisen ohjauksen toteutumista. Lapsen elinympäristö on perhe, ja vanhempien käsityksiä botuli…

CP-oireyhtymäFenomenografiabotuliinihoitospastisuusohjausfysioterapia
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Membrane vesicles shed by oligodendroglioma cells induce neuronal apoptosis.

2006

In order to investigate the mechanism by which oligodendrogliomas cause neuronal damage, media conditioned by G26/24 oligodendroglioma cells, were fractionated into shed vesicles and vesicle-free supernatants, and added to primary cultures of rat fetal cortical neurons. After one night treatment with vesicles, a reproducible, dose-dependent, inhibitory effect on neurite outgrowth was already induced and, after 48-72 h of incubation, neuronal apoptosis was evident. Vesicle-free supernatants and vesicles shed by NIH-3T3 cells had no inhibitory effects on neurons. Western blot analyses showed that treated neurons expressed a decreased amount of neurofilament (NF), growth-associated protein (GA…

Cancer ResearchCell signalingProgrammed cell deathPathologymedicine.medical_specialtyNeurofilamentFas Ligand ProteinNeuriteCellOligodendrogliomaApoptosisCell CommunicationBiologyRats Sprague-DawleyMiceWestern blotmedicineAnimalsMyelin SheathCerebral CortexNeuronsmedicine.diagnostic_testVesicleCytoplasmic Vesiclesoligodendroglioma membrane vesicles neuronal apoptosis Fas-L Nogo.Cell biologyRatsmedicine.anatomical_structureOncologyNIH 3T3 CellsNeuronInternational journal of oncology
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cIAP1 regulates TNF-mediated cdc42 activation and filopodia formation

2013

International audience; umour necrosis factor-α (TNF) is a cytokine endowed with multiple functions, depending on the cellular and environmental context. TNF receptor engagement induces the formation of a multimolecular complex including the TNFR-associated factor TRAF2, the receptor-interaction protein kinase RIP1 and the cellular inhibitor of apoptosis cIAP1, the latter being essential for NF-κB activation. Here, we show that cIAP1 also regulates TNF-induced actin cytoskeleton reorganization through a cdc42-dependent, NF-κB-independent pathway. Deletion of cIAP1 prevents TNF-induced filopodia and cdc42 activation. The expression of cIAP1 or its E3-ubiquitin ligase-defective mutant restore…

Cancer ResearchLung NeoplasmsBlotting WesternFluorescent Antibody Techniquemacromolecular substancesCDC42BiologyTransfectionInhibitor of Apoptosis ProteinsMice03 medical and health sciences0302 clinical medicineCell AdhesionGeneticsAnimalsHumansImmunoprecipitationNeoplasm InvasivenessPseudopodia[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronicscdc42 GTP-Binding ProteinMolecular Biology030304 developmental biology0303 health sciencesTumor Necrosis Factor-alphaActin cytoskeleton reorganizationCell PolarityActin remodelingSurface Plasmon ResonanceActin cytoskeletonCell biologyActin CytoskeletonDisease Models AnimalHEK293 CellsCdc42 GTP-Binding Protein030220 oncology & carcinogenesisNIH 3T3 CellsHeterografts[ SPI.NANO ] Engineering Sciences [physics]/Micro and nanotechnologies/MicroelectronicsPseudopodiaSignal transductionFilopodiaSignal TransductionOncogene
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