Search results for "NMT"
showing 6 items of 36 documents
Caracterización molecular de la anemia refractaria con sideroblastos en anillo mediante un panel de secuenciación de segunda generación
2019
Los síndromes mielodisplásicos (SMD) son un grupo heterogéneo de neoplasias hematológicas que presentan citopenias, mielodisplasia, hematopoyesis ineficaz y un riesgo variable de transformación a leucemia mieloide aguda (t-LMA). Los SMD con sideroblastos en anillo (SMD-SA) constituyen aproximadamente el 10-12% de todos los SMD y en médula ósea muestran SA, eritroblastos con depósitos anormales de ferritina, en porcentaje igual o superior al 5%. El objetivo terapéutico en los SMD-SA es mejorar las citopenias y su sintomatología, en especial el síndrome anémico. La administración de eritropoyetina y el soporte transfusional con concentrados de hematíes son las opciones terpéuticas de primera …
Cytosine methylation of tRNA-Asp by DNMT2 has a role in translation of proteins containing poly-Asp sequences
2015
AbstractThe Dnmt2 RNA methyltransferase catalyses the methylation of C38 in the anticodon loop of tRNA-Asp, but the molecular role of this methylation is unknown. Here, we report that mouse aspartyl-tRNA synthetase shows a four to fivefold preference for C38-methylated tRNA-Asp. Consistently, a 30% reduced charging level of tRNA-Asp was observed in Dnmt2 knockout (KO) murine embryonic fibroblast cells. Gene expression analysis with fluorescent reporter proteins fused to an N-terminal poly-Asp sequence showed that protein synthesis of poly-Asp-tagged reporter proteins was reduced in Dnmt2 KO cells as well. The same effect was observed with endogenous proteins containing poly-Asp sequences, i…
MiR-221 promotes stemness of breast cancer cells by targeting DNMT3b
2016
// Giuseppina Roscigno 1, 2 , Cristina Quintavalle 1, 2 , Elvira Donnarumma 3 , Ilaria Puoti 1 , Angel Diaz-Lagares 4 , Margherita Iaboni 1 , Danilo Fiore 1 , Valentina Russo 1 , Matilde Todaro 5 , Giulia Romano 6 , Renato Thomas 7 , Giuseppina Cortino 7 , Miriam Gaggianesi 5 , Manel Esteller 4 , Carlo M. Croce 6 , Gerolama Condorelli 1, 2 1 Department of Molecular Medicine and Medical Biotechnology, “Federico II” University of Naples, Naples, Italy 2 IEOS-CNR, Naples, Italy 3 IRCCS-SDN, Naples, Italy 4 Epigenetic and Cancer Biology Program (PEBC) IDIBELL, Hospital Duran I Reynals, Barcelona, Spain 5 Department of Surgical and Oncological Sciences, Cellular and Molecular Pathophysiology Lab…
Machine Translation and Gender biases in video game localisation: a corpus-based analysis
2021
The video game industry has been a historically gender-biased terrain due to a higher number of male protagonists and hypersexualised representations [Dietz, 1998; Downs & Smith, 2010; Lynch et al., 2016]. Nowadays, echoing the debate on inclusive language, companies attempt to erase gender disparity by introducing main female characters as well as non-binary characters. From a technological point of view, even though recent studies show that Machine Translation remains largely unadopted by individual video game localisers [Rivas Ginel, 2021], multilanguage vendors are willing to invest in these tools to reduce costs [LIND, 2020]. However, the predominance of the masculine in Natural La…
Dzimtes stereotipu mazināšana mašīntulkošanā: mērķvalodas gramatiskās dzimtes projekcijas avotvalodā
2020
Tulkojot “The secretary asked for details.” no valodas bez gramatiskās dzimtes (angļu) uz valodu ar gramatisko dzimti (piemēram, latviešu), nepieciešams noteikt subjekta “the secretary” dzimumu. Ja teikuma līmenī nav nepieciešamās informācijas, to ne vienmēr ir iespējams izdarīt. Šādos gadījumos mašīntulkošanas sistēmas izvēlās biežāk sastopamos un līdz ar to dzimtes stereotipiem atbilstošos tulkojumu variantus (t.i. “sekretāre”). Darbā tiek piedāvāta mašīntulkošanas sistēmu apmācības metode, kur avotvalodas vārdiem ir tieši norādīta dzimtes pazīme (sieviešu vai vīriešu). Sistēmu apmācības dati tiek sagatavoti, projicējot mērķvalodas vārdu gramatiskās dzimtes uz avotvalodas vārdiem. Rezultā…
Effects of 5-Azacitidine on Dnmt2/Trdmt1 expression levels and endoplasmic reticulum stress in cellular models of insulinoma
2021
Diabetes mellitus affects people all over the world of all ages or social groups making it a worldwide healthcare challenge. Moreover, untreated or badly treated diabetes carries a risk of serious complications including premature death (IDF Diabetes Atlas 9th edition, 2019). The main symptom of diabetes is insulin secretion and/or action disorder leading to hyperglycemia what results in impaired carbohydrate, fat, and protein metabolism (“Diagnosis and Classification of Diabetes Mellitus,” 2013). Dnmt2/ Trdmt1 in its structure and sequence is similar to DNA methyltransferases, however, it has been shown that mainly methylates aspartic acid transfer RNA, specifically at the cytosine-38 resi…