Search results for "Nephropidae"
showing 10 items of 15 documents
Lobster and cod benefit from small-scale northern marine protected areas: inference from an empirical before - after control-impact study
2013
Published version of an article from the journal: Proceedings of the Royal Society B-Biological Sciences. Alsop available from the Royal Society: http://dx.doi.org/10.1098/rspb.2012.2679 Open Access Marine protected areas (MPAs) are increasingly implemented as tools to conserve and manage fisheries and target species. Because there are opportunity costs to conservation, there is a need for science-based assessment of MPAs. Here, we present one of the northernmost documentations of MPA effects to date, demonstrated by a replicated before-after control-impact (BACI) approach. In 2006, MPAs were implemented along the Norwegian Skagerrak coast offering complete protection to shellfish and parti…
Conservation, Spillover and Gene Flow within a Network of Northern European Marine Protected Areas
2013
Published version of an article in the journal: PLoS ONE. Also available from the publisher at: http://dx.doi.org/10.1371/journal.pone.0073388 Open access To ensure that marine protected areas (MPAs) benefit conservation and fisheries, the effectiveness of MPA designs has to be evaluated in field studies. Using an interdisciplinary approach, we empirically assessed the design of a network of northern MPAs where fishing for European lobster (Homarus gammarus) is prohibited. First, we demonstrate a high level of residency and survival (50%) for almost a year (363 days) within MPAs, despite small MPA sizes (0.5-1 km2). Second, we demonstrate limited export (4.7%) of lobsters tagged within MPAs…
Identification, molecular cloning, and phylogenetic analysis of a non-respiratory pseudo-hemocyanin of Homarus americanus.
1999
Copper-containing hemocyanins serve to transport oxygen in many arthropod species. Here I describe the identification and cDNA cloning of a structurally closely related non-respiratory pseudo-hemocyanin (PHc) of the American lobster, Homarus americanus. This protein has lost the ability to bind copper and, therefore, oxygen because a histidine residue in copper-binding site A is replaced by tyrosine. Like many arthropod hemocyanins, PHc forms a hexamer. It consists of two different subunit types of 660 and 661 amino acids, respectively, that share a 94.4% sequence identity. Whereas Homarus hemocyanin is produced in the hepatopancreas, PHc is synthesized by the ovaries and the heart tissue. …
Allosteric Models for Multimeric Proteins: Oxygen-Linked Effector Binding in Hemocyanin
2005
In many crustaceans, changing concentrations of several low molecular weight compounds modulates hemocyanin oxygen binding, resulting in lower or higher oxygen affinities of the pigment. The nonphysiological effector caffeine and the physiological modulator urate, the latter accumulating in the hemolymph of the lobster Homarus vulgaris during hypoxia, increase hemocyanin oxygen affinity and decrease cooperativity of oxygen binding. To derive a model that describes the mechanism of allosteric interaction between hemocyanin and oxygen in the presence of urate or caffeine, studies of oxygen, urate, and caffeine binding to hemocyanin were performed. Exposure of lobster hemocyanin to various pH …
Small-signal analysis of the encoder mechanism in the lobster stretch receptor and the frog and cat muscle spindle.
1975
Ausgehend von der Leitwert-Potential-Beziehung als Bedingung fur die Impulsauslosung an erregbaren Membranen (Chaplain, 1974) wird eine Kleinsignal-Analyse des Kodierungsvorganges in Mechanorezeptoren beschrieben. Hierbei wird die Theorie der Systeme mit zeitvariablen Parametern angewendet. Das beschriebene mathematische Modell erlaubt quantitative Aussagen fur den Frequenzgang unabhangig davon ob die Momentafrequenz oder die Impulsdichte als Ausgangssignal betrachtet wird. Die Approximation experimenteller Daten gelingt sowohl fur die Ansteuerung des Kodierers bei intrazellularer Stromstimulierung im Fall des Dehnungsrezeptors wie auch durch Leitwertanderungen als Folge des mechano-elektri…
Genotype Reconstruction of Paternity in European Lobsters (Homarus gammarus)
2015
Decapod crustaceans exhibit considerable variation in fertilisation strategies, ranging from pervasive single paternity to the near-ubiquitous presence of multiple paternity, and such knowledge of mating systems and behaviour are required for the informed management of commercially-exploited marine fisheries. We used genetic markers to assess the paternity of individual broods in the European lobster, Homarus gammarus, a species for which paternity structure is unknown. Using 13 multiplexed microsatellite loci, three of which are newly described in this study, we genotyped 10 eggs from each of 34 females collected from an Atlantic peninsula in the south-western United Kingdom. Single recons…
Quaternary and subunit structure of Calliphora arylphorin as deduced from electron microscopy, electrophoresis, and sequence similarities with arthro…
1992
Arylphorin was purified from larvae of the blowfly Calliphora vicina and studied in its oligomeric form and after dissociation at pH 9.6 into native subunits. In accordance with earlier literature, it was electrophoretically shown to be a 500 kDa hexamer (1 x 6) consisting of 78 kDa polypeptides (= subunits). Electron micrographs of negatively stained hexamers show a characteristic curvilinear, equilateral triangle of 12 nm in diameter (top view) and a rectangle measuring 10 x 12 nm (side view). Alternatively, particles in the top view orientation exhibit a roughly circular shape 12 nm in diameter. Crossed immunoelectrophoresis revealed the presence of a major subunit type; the nature of a …
A STUDY OF POPULATION GENETICS IN THE EUROPEAN LOBSTER, HOMARUS GAMMARUS (DECAPODA, NEPHROPIDAE)
2001
By means of an RAPD analysis, the degree of genetic variation in the European lobster, Homarus gammarus, was assessed. We focused our attention on the Helgoland population, which is isolated by its geographical location. By comparison with seven other European lobster populations we demonstrated that the Helgoland lobster is genetically widely separated from other European lobster stocks. However, all populations investigated showed high values of genetic distance, indicating that genetic exchange among European populations of Homarus gammarus may be restricted. The European lobster populations appeared to be isolated by distance since the pattern of genetic relationships reflects their geo…
Influence of antibody binding on oxygen binding behavior of Panulirus interruptus hemocyanin
1997
Oxygen binding behavior of monomeric subunit a and the hexameric form of this subunit of hemocyanin of Panulirus interruptus is influenced by the binding of various monoclonal antibodies. These antibodies react with other surface parts of the subunit than its second domain in which the oxygen binding site is located. The influence of three monoclonal antibodies and their antigen binding fragments (F-ab) has been investigated. Two antibodies increase the oxygen affinity of monomeric hemocyanin from that observed in its low affinity T-state, while the third has little influence on this property. F-ab fragments abolish almost completely the cooperativity of oxygen binding by the hexameric hemo…
Binding of urate and caffeine to hemocyanin of the lobster Homarus vulgaris (E.) as studied by isothermal titration calorimetry.
2000
Hemocyanin serves as an oxygen carrier in the hemolymph of the European lobster Homarus vulgaris. The oxygen binding behavior of the pigment is modulated by metabolic effectors such as lactate and urate. Urate and caffeine binding to 12-meric hemocyanin (H. vulgaris) was studied using isothermal titration calorimetry (ITC). Binding isotherms were determined for fully oxygenated hemocyanin between pH 7.55 and 8.15. No pH dependence of the binding parameters could be found for either effector. Since the magnitude of the Bohr effect depends on the urate concentration, the absence of any pH dependence of urate and caffeine binding to oxygenated hemocyanin suggests two conformations of the pigme…