Search results for "Nicotiana"
showing 4 items of 84 documents
Silenciamiento sistémico inducido por pequeños RNAs artificiales en Nicotiana benthamiana
2020
Los pequeños RNAs (sRNAs), microRNAs (miRNAs) y sRNAs de interferencia (siRNAs), degradan específicamente moléculas de RNA de secuencia complementaria, proceso conocido como silenciamiento génico. En algunos casos, los miRNAs de 22 nucleótidos inducen la producción de siRNAs secundarios a partir sus RNAs diana, y estos siRNAs secundarios se desplazan por la planta a corta y larga distancia propagando el silenciamiento. Experimentos previos mostraron que la expresión transitoria en hojas de Nicotiana benthamiana del miRNA artificial (amiRNA) amiR-NbSu-2 de 21 nucleótidos frente al gen SULPHUR inducía silenciamiento sistémico (SS) en hojas jóvenes no agroinfiltradas, además de silenciamiento …
Drought Stress Study on Nicotiana tabacum L., “Baladi”, an In Vitro Experimental Model
2021
Crops drought tolerance is a trait of outmost importance for agriculture especially today when climate change is affecting more the production for food and feed. The scope of this article is to evaluate in vitro drought stress response of Nicotiana tabacum L., “Baladi”. The experiment was set up for four successive stages starting with in vitro seedling development, hypocotyl cultivation, three generations of micropropagation, pre-acclimatization and acclimatization. The effect of abscisic acid (ABA) and/or polyethylene-glycol 6000 (PEG) on tobacco hypocotyl caulogenesis and micropropagation were investigated. Superoxide-dismutases (SODs) and peroxidases (POXs) are more active and different…
Adventiv-Embryonen aus Nicotiana-kallus, der bei Hohen Lichtintensitäten Kultiviert wurde
1965
The formation of embryonic structures in Nicotiana callus tissue has been reported. High intensity of light as a stimulating factor in differentiation is discussed.
Single-chain antibodies against a plant viral RNA-dependent RNA polymerase confer virus resistance.
2004
Crop loss due to viral diseases is still a major problem for agriculture today. We present a strategy to achieve virus resistance based on the expression of single-chain Fv fragments (scFvs) against a conserved domain in a plant viral RNA-dependent RNA polymerase (RdRp), a key enzyme in virus replication. The selected scFvs inhibited complementary RNA synthesis of different plant virus RdRps in vitro and virus replication in planta. Moreover, the scFvs also bound to the RdRp of the distantly related hepatitis C virus. T(1) and T(2) progeny of transgenic lines of Nicotiana benthamiana expressing different scFvs either in the cytosol or in the endoplasmic reticulum showed varying degrees of r…