Search results for "Non-target"
showing 6 items of 16 documents
Genetic determinisms, evolution and detection of non-target-site based resistance to herbicides inhibiting acetolactate-synthase in rye-grass (Lolium…
2016
The aim of this study is to unravel the genetic determinism of non-target-site resistance (NTSR) to herbicides inhibiting acetolactate-synthase (ALS) in the major weed rye-grass (Lolium sp.), and to estimate the feasibility of NTSR diagnosis based on genetic data. On the one hand, this work contributes to the understanding of the processes driving the evolution of NTSR to herbicides, and on the other hand it lays the foundations for the development of a diagnosis tool to identify NTSR to ALS inhibiting herbicides. - Using a quantitative transcriptome sequencing approach, we showed that NTSR evolve by recurrent selection of higher and higher constitutive expression levels of genes involved i…
DIAGSOL : development of a new functional marker of exposure to herbicides B-triketones in an agricultural soil
2020
Numerous herbicides target an enzyme found not only in weeds but also in « non-target organisms » such as microorganisms. This proof-of-concept study aims to use microbial gene encoding the targeted enzyme or the targeted enzyme itself as a marker for herbicide exposure in soils. The hppd gene and the encoded enzyme (HPPD; 4-hydroxyphenylpyruvate dioxygenase), targeted by B-triketone herbicides, are the subject of this study. In silico analyses reveal that the hppd gene is spread out in all bacterial phyla. Primers specific to this gene were designed. This primer pair is used to measure the abundance, the composition and the diversity of the hppd bacterial community in soil microcosms expos…
Identification de gènes liés à la résistance aux herbicides non liée à la cible chez les Ivraies : vers des tests « moléculaires » de diagnostic
2016
Identification of genes linked to non-target-site resistance to herbicides in ryegrass : towards "molecular" diagnosis assays. Setting up molecular diagnosis tools would greatly foster preventing and managing non-target-sitebased resistance to herbicides (NTSR). This is currently hampered by the complexity of NTSR genetic determinism, which is largely based upon differences in the expression of an unknown number of genes between resistant and sensitive plants. Identifying NTSR genes is thus a challenging task. Herein, we report the identification of 19 genes which expression patterns are correlated to NTSR to ALS-inhibiting herbicides in rye-grasses (Lolium sp.). We demonstrate the potentia…
Conséquences de l’utilisation de variétés de tournesol tolérantes aux herbicides sur la flore des agrosystèmes
2022
The farming practices associated with the use of sunflower herbicide-tolerant varieties (HTVs) have raised concerns among public authorities, who have recommended monitoring the potential effects of HTVs on biodiversity. In this context, at the scale of three French departments, the vegetation of 239 sunflower fields and their margins was studied between 2017 and 2019. This lower weed diversity can be attributed to shorter crop rotations and higher herbicide use. Admittedly, the higher densities of A. artemisiifolia observed in plots with HTVs are the cause of the use of these varieties rather than the consequence of these practices. However, these practices do not seem to solve the problem…
Les phytoprotecteurs (« Safeners ») associés aux herbicides jouent-ils un rôle dans la sélection de résistances non liées à la cible ?
2016
Do herbicide-associated "Safeners" play a role in the selection for non-target-site resistances ? To ensure or improve crop selectivity, some herbicide molecules are applied in association with a “safener”. Safeners are compounds that, at the rate applied, enhance herbicide-degrading metabolic pathways supposedly specifically in crop plants. The metabolic pathways enhanced by safeners in crop plants are highly similar to those exacerbated in taxonomically closely related weed species that evolved non-target-site based resistance (NTSR) to herbicides. Herein, we show an enhancing effect of herbicide safeners on NTSR in a major noxious grass weed: rye-grass (Lolium sp.).
Universal method to determine acidic licit and illicit drugs and personal care products in water by liquid chromatography quadrupole time-of-flight
2016
Graphical abstract