Search results for "OMEGA"

showing 10 items of 1174 documents

Right ventricular collagen type III and IV gene expression increases during early phases of endurance training in hypobaric hypoxic condition

1997

The objective of this study was to examine the effects of prolonged exposure to hypobaric hypoxic condition, physical training and their combination on collagen type I, III and IV gene expression in the ventricles and atria of rat heart. Male rats were assigned to four groups: normobaric sedentary (NS) and trained (NT), and hypobaric sedentary (HS) and trained (HT). Exposure to and treadmill running training in hypobaric condition were carried out in a hypobaric chamber (770–740 mbar, 2250–2550 m). Experimental periods were 10, 21 and 56 days; the groups of 91 days served as recovery groups from experimental settings of 56 days. Exposure to hypobaric condition as such and in combination wit…

MaleAtmosphere Exposure Chambersmedicine.medical_specialtyPhysiologyHeart VentriclesProcollagen-Proline DioxygenaseGene ExpressionCardiomegalyBiologyLeft ventricular hypertrophyCollagen Type IIIEndurance trainingRight ventricular hypertrophyPhysical Conditioning AnimalPhysiology (medical)Internal medicineGene expressionmedicineAnimalsHeart AtriaRNA MessengerRats WistarHypoxiaCardiac muscleBlotting Northernmedicine.diseaseRatsHydroxyprolineAtmospheric PressureEndocrinologymedicine.anatomical_structureMrna levelHypobaric chamberPhysical EnduranceCollagenCardiology and Cardiovascular MedicineBasic Research in Cardiology
researchProduct

The Major Virus-Producing Cell Type during Murine Cytomegalovirus Infection, the Hepatocyte, Is Not the Source of Virus Dissemination in the Host

2008

SummaryThe course of systemic viral infections is determined by the virus productivity of infected cell types and the efficiency of virus dissemination throughout the host. Here, we used a cell-type-specific virus labeling system to quantitatively track virus progeny during murine cytomegalovirus infection. We infected mice that expressed Cre recombinase selectively in vascular endothelial cells or hepatocytes with a murine cytomegalovirus for which Cre-mediated recombination would generate a fluorescently labeled virus. We showed that endothelial cells and hepatocytes produced virus after direct infection. However, in the liver, the main contributor to viral load in the mouse, most viruses…

MaleCancer ResearchCell typeMuromegalovirusMICROBIOvirusesGreen Fluorescent ProteinsCongenital cytomegalovirus infectionCre recombinaseViral transformationMice TransgenicBiologyVirus ReplicationMicrobiologyVirusMicrobiologyCell LineMiceImmunology and Microbiology(all)VirologymedicineAnimalsMolecular BiologyRecombination GeneticIntegrasesViral cultureEndothelial CellsHerpesviridae InfectionsFibroblastsmedicine.diseaseVirologyMice Inbred C57BLmedicine.anatomical_structureLiverHepatocyteHepatocytesParasitologyFemaleCELLBIOViral loadCell Host & Microbe
researchProduct

The ISWI chromatin remodeler organizes the hsrω ncRNA-containing omega speckle nuclear compartments.

2011

The complexity in composition and function of the eukaryotic nucleus is achieved through its organization in specialized nuclear compartments. The Drosophila chromatin remodeling ATPase ISWI plays evolutionarily conserved roles in chromatin organization. Interestingly, ISWI genetically interacts with the hsrω gene, encoding multiple non-coding RNAs (ncRNA) essential, among other functions, for the assembly and organization of the omega speckles. The nucleoplasmic omega speckles play important functions in RNA metabolism, in normal and stressed cells, by regulating availability of hnRNPs and some other RNA processing proteins. Chromatin remodelers, as well as nuclear speckles and their assoc…

MaleCancer ResearchRNA Untranslatedlcsh:QH426-470Gene ExpressionFluorescent Antibody TechniqueRNA-binding proteinBiologyEyeHeterogeneous ribonucleoprotein particleChromosomesHeterogeneous-Nuclear RibonucleoproteinsChromatin remodelingMolecular GeneticsGeneticsmedicineAnimalsDrosophila ProteinsOmega speckleBiologyMolecular BiologyTranscription factorAllelesGenetics (clinical)Ecology Evolution Behavior and SystematicsAdenosine TriphosphatasesCell NucleusGeneticsRNA-Binding ProteinsEpistasis GeneticChromatin Assembly and DisassemblyNon-coding RNAChromatinCell biologyCell nucleuslcsh:GeneticsPhenotypemedicine.anatomical_structureTandem Repeat SequencesChromatin remodeling non coding RNALarvaEpigeneticsDrosophilaRNA InterferenceResearch ArticleTranscription FactorsPLoS Genetics
researchProduct

The bite force and craniofacial morphology in patients with acromegaly: A pilot study

2013

Objectives: Acromegaly is a metabolic disorder caused by increased growth hormone secretion. As a consequence of acromegaly some typical craniofacial morphology changes appear. This pilot study was conducted to compare the bite force and the characteristic size and shape of the craniofacial components of acromegalic patients with the healthy Turkish individuals. In additon, the correlations between bite force and craniofacial morphology of patients with acromegaly and control individuals were evaluated. Study Design: The maximum bite force of the participants was recorded with strain-gage transducer. Lateral xray scans were made under standard conditions, in centric occlusion. On cephalogra…

MaleCephalometryCraniofacial abnormalityDentistryPilot ProjectsOdontologíaBite ForceCraniofacial AbnormalitiesAcromegalyHumansMedicineGonial angleCraniofacialGeneral DentistryOral Medicine and Pathologybusiness.industryResearchMandibleCraniometrymedicine.disease:CIENCIAS MÉDICAS [UNESCO]Ciencias de la saludBite force quotientSella turcicamedicine.anatomical_structureOtorhinolaryngologyAcromegalyUNESCO::CIENCIAS MÉDICASFemaleSurgerybusiness
researchProduct

Mast cells as rapid innate sensors of cytomegalovirus by TLR3/TRIF signaling-dependent and -independent mechanisms

2014

The succinct metaphor, ‘the immune system's loaded gun', has been used to describe the role of mast cells (MCs) due to their storage of a wide range of potent pro-inflammatory and antimicrobial mediators in secretory granules that can be released almost instantly on demand to fight invaders. Located at host–environment boundaries and equipped with an arsenal of pattern recognition receptors, MCs are destined to be rapid innate sensors of pathogens penetrating endothelial and epithelial surfaces. Although the importance of MCs in antimicrobial and antiparasitic defense has long been appreciated, their role in raising the alarm against viral infections has been noted only recently. Work on cy…

MaleChemokineImmunologyCytomegalovirusBiologyCD8-Positive T-LymphocytesCCL5MiceImmune systemImmunology and AllergyCytotoxic T cellAnimalsMast CellsMice KnockoutIntegrasesMacrophagesDegranulationPattern recognition receptorhumanitiesToll-Like Receptor 3Killer Cells NaturalMice Inbred C57BLAdaptor Proteins Vesicular TransportInfectious DiseasesTRIFImmunologyTLR3Cytomegalovirus Infectionsbiology.proteinFemaleResearch Article
researchProduct

Development and initial validation of a composite disease activity score for systemic juvenile idiopathic arthritis

2020

Made available in DSpace on 2021-06-25T10:38:01Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-11-01 Healthway Objective. To develop a composite disease activity score for systemic JIA (sJIA) and to provide preliminary evidence of its validity. Methods. The systemic Juvenile Arthritis Disease Activity Score (sJADAS) was constructed by adding to the four items of the original JADAS a fifth item that aimed to quantify the activity of systemic features. Validation analyses were conducted on patients with definite or probable/possible sJIA enrolled at first visit or at the time of a flare, who had active systemic manifestations, which should include fever. Patients were reassessed 2 wee…

MaleClinical assessment; Composite disease activity score; Disease activity; Outcome measures; Pediatric rheumatology; Still's disease; Systemic juvenile idiopathic arthritismedicine.medical_specialtyFeverClinical assessmentComposite disease activity scoreArthritisLymphadenopathyDiseaseSeverity of Illness IndexOutcome measuresOutcome measureJuvenile Arthritis Disease Activity ScoreRheumatologyCronbach's alphaSystemic juvenile idiopathic arthritisInternal medicineStill's diseaseContent validityMedicineJuvenileHumansPharmacology (medical)Pediatric rheumatologyDisease activityRange of Motion ArticularChildPain MeasurementSerositisThrombocytosisbusiness.industryConstruct validityReproducibility of ResultsAnemiaExanthemamedicine.diseaseArthralgiaRheumatologyArthritis JuvenileChild PreschoolSplenomegalyQuality of LifeFemaleHyperferritinemiabusinessHepatomegaly
researchProduct

Ablation of the Regulatory IE1 Protein of Murine Cytomegalovirus Alters In Vivo Pro-inflammatory TNF-alpha Production during Acute Infection

2012

Little is known about the role of viral genes in modulating host cytokine responses. Here we report a new functional role of the viral encoded IE1 protein of the murine cytomegalovirus in sculpting the inflammatory response in an acute infection. In time course experiments of infected primary macrophages (MΦs) measuring cytokine production levels, genetic ablation of the immediate-early 1 (ie1) gene results in a significant increase in TNFα production. Intracellular staining for cytokine production and viral early gene expression shows that TNFα production is highly associated with the productively infected MΦ population of cells. The ie1- dependent phenotype of enhanced MΦ TNFα production …

MaleCytomegalovirus InfectionMuromegalovirusViral Diseasesmedicine.medical_treatmentvirusesTNF TNF-alpha murine cytomegalovirus MCMV IEVirus ReplicationMice0302 clinical medicineGene expressionBiology (General)Mice Inbred BALB C0303 health scienceseducation.field_of_studyPhysicsvirus diseasesHerpesviridae InfectionsTransfection3. Good healthGenètica microbianaInterleukin 10PhenotypeInfectious DiseasesCytokineLiverCytokinesMedicineFemaleTumor necrosis factor alphaBIOMEDICINA I ZDRAVSTVO. Temeljne medicinske znanosti.Microbial geneticsSignal TransductionResearch ArticleDNA ReplicationGene Expression Regulation ViralQH301-705.5ImmunologyPopulationBiologyMicrobiologyCell LineImmediate-Early ProteinsViral Proteins03 medical and health sciencesIn vivoVirologyGeneticsmedicineAnimalseducationMolecular Biology030304 developmental biologyTumor Necrosis Factor-alphaMacrophagesBIOMEDICINE AND HEALTHCARE. Basic Medical Sciences.FísicaRC581-607Mice Inbred C57BLViral replicationDNA ViralImmunologyParasitologyImmunologic diseases. Allergy030215 immunology
researchProduct

Effect of cytomegalovirus-induced immune response, self antigen-induced immune response, and microbial translocation on chronic immune activation in …

2013

International audience; We evaluated the impact of cytomegalovirus (CMV)-induced immune responses, autoimmune-induced immune responses, and microbial translocation on immune activation in 191 human immunodeficiency virus type 1-infected patients from the ANRS CO3 Aquitaine Cohort. All enrolled subjects had achieved long-term virological suppression during receipt of combination antiretroviral therapy (cART). HLA-DR(+)/CD38(+) expression was 16.8% among CD8(+) T cells. Independent of age, CD4(+) T-cell count, 16S ribosomal DNA load, and regulatory T-cell count, positive results of Quantiferon CMV analysis (P = .02), positive results of CMV-pp65 enzyme-linked immunosorbent spot analysis (P = …

MaleCytomegalovirusAutoimmunityHIV InfectionsCD8-Positive T-LymphocytesCD38Lymphocyte Activationmedicine.disease_causeAutoimmunityCohort Studies0302 clinical medicine[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases[ SDV.MP ] Life Sciences [q-bio]/Microbiology and ParasitologyAntiretroviral Therapy Highly ActiveImmunology and Allergy030212 general & internal medicineMESH: Cytomegalovirus Infections/immunologyMESH: HLA-DR Antigens/metabolismMESH: Cohort Studies0303 health sciencesMESH: HIV Infections/drug therapyvirus diseasesViral Load3. Good healthInfectious Diseases[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologyMESH: CD8-Positive T-Lymphocytes/immunologyCytomegalovirus Infections[SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases[SDV.IMM]Life Sciences [q-bio]/ImmunologyFemaleFranceMESH: Cytomegalovirus/immunologyMESH: Viral Load[SDV.IMM] Life Sciences [q-bio]/ImmunologyCongenital cytomegalovirus infectionHuman leukocyte antigenBiologyQuantiFERONViral Matrix Proteins03 medical and health sciencesImmune systemMESH: Cross-Sectional StudiesAntigenMESH: AutoimmunitymedicineHumansMESH: Phosphoproteins/immunology[SDV.MP] Life Sciences [q-bio]/Microbiology and ParasitologyMESH: Viral Matrix Proteins/immunology030304 developmental biologyMESH: HumansMESH: HIV-1/geneticsMESH: HIV-1/physiologyMESH: HIV Infections/immunologyHLA-DR AntigensPhosphoproteinsmedicine.diseaseVirologyMESH: MaleMESH: Lymphocyte Activation/immunologyMESH: FranceCross-Sectional Studies[SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologieImmunologyHIV-1[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologieMESH: HIV-1/drug effectsMESH: FemaleCD8
researchProduct

Downregulation and Nuclear Relocation of MLP During the Progression of Right Ventricular Hypertrophy Induced by Chronic Pressure Overload

2000

Abstract The cardiac LIM domain protein MLP plays a crucial role in the architecture and mechanical function of cardiac myocytes. Mice lacking the MLP gene develop cardiac hypertrophy, dilated cardiopathy and heart failure. We investigated whether downregulation of MLP is induced by pressure overload and contributes to the physiopathology of cardiac hypertrophy and failure. We studied this mechanism in rat right ventricles submitted to pulmonary arterial hypertension, because it is known that this ventricle is very vulnerable to the deleterious effects of pressure overload. During the progression of cardiac hypertrophy to failure over a 31 days period there was a dramatic decrease by 50% of…

MaleCytoplasmmedicine.medical_specialtyTime FactorsTranscription GeneticHeart VentriclesDown-RegulationMuscle ProteinsCardiomegalyCytosolMyofibrilsDownregulation and upregulationRight ventricular hypertrophyInternal medicinePressureAnimalsVentricular FunctionMedicineMyocyteRNA MessengerRats WistarLungMolecular BiologyCell NucleusHomeodomain ProteinsPressure overloadReverse Transcriptase Polymerase Chain Reactionbusiness.industryMyocardiumLIM Domain Proteinsmedicine.diseaseImmunohistochemistryPulmonary hypertensionRatsMicroscopy Electronmedicine.anatomical_structureVentricleHeart failureCardiologyCardiology and Cardiovascular MedicinebusinessMyofibrilJournal of Molecular and Cellular Cardiology
researchProduct

Epidemiology of Toxoplasma and CMV serology and of GBS colonization in pregnancy and neonatal outcome in a Sicilian population

2013

Background: Aim of our study is to analyze the immunological status in pregnancy for two main TORCH agents, Toxoplasma and Cytomegalovirus (CMV), and the results of group B streptococcus (GBS) screening, assessing the risk for congenital infection in a population from Palermo, Italy. Methods: We retrospectively analyzed the medical records of all inborn live newborns who were born in our division during 2012, gathering information about the mother, the pregnancy and neonatal hospitalization at birth. Whenever data were available, we categorized the serologic status of the mothers for Toxoplasma and CMV. We also considered the results of rectal and vaginal swabs for GBS. We compared the resu…

MaleEpidemiologyAntibodies ProtozoanCytomegalovirusmedicine.disease_causeAntibodies ViralGroup BInfant Newborn DiseasesSerologySettore MED/38 - Pediatria Generale E SpecialisticaPregnancyRisk FactorsPrenatal DiagnosisEpidemiologyPrevalencePregnancy Complications InfectiousIntrauterine infectionSicilyeducation.field_of_studyPregnancy OutcomeCMVMiddle AgedAntibodies BacterialAntibodies Anti-IdiotypicCytomegalovirus InfectionsFemaleToxoplasmaToxoplasmosisAdultmedicine.medical_specialtyAdolescentPopulationCongenital cytomegalovirus infectionGBSRisk AssessmentStreptococcus agalactiaeYoung AdultStreptococcal InfectionsmedicineAnimalsHumanseducationRetrospective StudiesPregnancybusiness.industryResearchInfant Newbornmedicine.diseaseToxoplasmosisStreptococcus agalactiaeImmunoglobulin GImmunologybusinessItalian Journal of Pediatrics
researchProduct