Search results for "OSA"

showing 10 items of 9388 documents

The effects of prebiotics on microbial dysbiosis, butyrate production and immunity in HIV-infected subjects

2017

Altered interactions between the gut mucosa and bacteria during HIV infection seem to contribute to chronic immune dysfunction. A deeper understanding of how nutritional interventions could ameliorate gut dysbiosis is needed. Forty-four subjects, including 12 HIV+ viremic untreated (VU) patients, 23 antiretroviral therapy-treated (ART(+)) virally suppressed patients (15 immunological responders and 8 non-responders) and 9 HIV- controls (HIV-), were blindly randomized to receive either prebiotics (scGOS/lcFOS/glutamine) or placebo (34/10) over 6 weeks in this pilot study. We assessed fecal microbiota composition using deep 16S rRNA gene sequencing and several immunological and genetic marker…

AdultMale0301 basic medicine030106 microbiologyImmunologyHIV InfectionsInflammationButyrateBiologyGut floraMicrobiologyFeces03 medical and health sciencesIntestinal mucosaImmunityRNA Ribosomal 16SmedicineHumansImmunology and AllergyIntestinal MucosaBacteriaImmunityMiddle AgedPlacebo Effectmedicine.diseasebiology.organism_classificationGastrointestinal MicrobiomeGlutamineButyratesPrebiotics030104 developmental biologyMucosal immunologyDietary SupplementsHost-Pathogen InteractionsImmunologyHIV-1DysbiosisFemalemedicine.symptomDysbiosisMucosal Immunology
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Homozygous variants in the gene SCAPER cause syndromic intellectual disability

2019

The S-Phase Cyclin A Associated Protein In The ER (SCAPER) gene is a ubiquitously expressed gene with unknown function in the brain. Recently, biallelic SCAPER variants were described in four patients from three families with retinitis pigmentosa (RP) and intellectual disability (ID). Here, we expand the spectrum of pathogenic variants in SCAPER and report on 10 further patients from four families with ID, RP, and additional dysmorphic features carrying homozygous variants in SCAPER. The variants found comprise frameshift, nonsense, and missense variants as well as an intragenic homozygous deletion, which spans SCAPER exons 15 and 16 and introduces a frameshift and a premature stop codon. A…

AdultMale0301 basic medicineAdolescentmedia_common.quotation_subjectCyclin ANonsenseGene Expression030105 genetics & heredityFrameshift mutationConsanguinityMice03 medical and health sciencesExonNeural Stem CellsIntellectual DisabilityRetinitis pigmentosaGene expressionGeneticsmedicineAnimalsHumansMissense mutationFamilyChildGeneGenetics (clinical)media_commonCerebral CortexNeuronsGeneticsbiologyHomozygoteSyndromemedicine.diseasePedigree030104 developmental biologyMutationbiology.proteinFemaleCarrier ProteinsRetinitis PigmentosaAmerican Journal of Medical Genetics Part A
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Characterization of γδ T cells in intestinal mucosa from patients with early onset or long standing inflammatory bowel disease and their correlation …

2019

Abstract Background and Aims Inflammatory bowel disease [IBD] is a complex chronic inflammatory disease of the human gut with no clear aetiology. Traditionally, dysregulated adaptive immune responses play an important role even though accumulating evidence suggests a role also for innate immunity. Because of the well-known plasticity of γδ T cells, we investigated their percentage occurrence, phenotypic features and effector functions in the intestinal mucosa of early-onset and long-standing IBD patients, as compared to healthy subjects. Methods Fresh biopsies from 30 Crohn’s disease and ulcerative colitis patients were obtained and digested, and cells were analysed by flow cytometry. Resul…

AdultMale0301 basic medicineNecrosisAdolescentBiopsyT-LymphocytesInflammatory bowel disease03 medical and health sciences0302 clinical medicineImmune systemIntestinal mucosainflammatory bowel diseasemedicineHumansgamma delta T cellsIntestinal MucosaAgedAged 80 and overInnate immune systembusiness.industryGastroenterologyGeneral MedicineMiddle AgedFlow CytometryInflammatory Bowel Diseasesmedicine.diseaseUlcerative colitisPhenotype030104 developmental biologyImmunologyFemaleTumor necrosis factor alphaInterleukin 17medicine.symptombusinessBiomarkers030215 immunology
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Colorectal cancer with microsatellite instability: Right-sided location and signet ring cell histology are associated with nodal metastases, and extr…

2021

Colorectal cancer (CRC) with microsatellite instability (MSI) accounts for 15-18 % of all CRCs and represents the category with the best prognosis. This study aimed at determining any possible clinical/pathological features associated with a higher risk of nodal metastasization in MSI-CRC, and at defining any possible prognostic moderators in this setting. All surgically resected CRCs of the last 20 years (mono-institutional series) with a PCR-based diagnosis of MSI, with and without nodal metastasis, have been retrieved for histological review, which was performed following WHO guidelines. Furthermore, the most important prognostic moderators have been investigated with a survival analysis…

AdultMale0301 basic medicineOncologycongenital hereditary and neonatal diseases and abnormalitiesmedicine.medical_specialtyPrognostic variableColonColorectal cancerDisease-Free SurvivalMetastasisPathology and Forensic MedicineMetastasis03 medical and health sciences0302 clinical medicineInternal medicinedMMRmedicinerectumHumansStage (cooking)neoplasmsMSISurvival analysisAgedColon; ENE; Extracapsular; MSI; Metastasis; dMMR; rectumExtranodal ExtensionExtracapsularSignet ring cellbusiness.industryColon; dMMR; ENE; Extracapsular; Metastasis; MSI; rectumMicrosatellite instabilityCell BiologyMiddle AgedPrognosismedicine.diseaseProgression-Free Survivaldigestive system diseases030104 developmental biology030220 oncology & carcinogenesisENEFemaleMicrosatellite InstabilityColorectal NeoplasmsNODALbusinessCarcinoma Signet Ring CellPathology - Research and Practice
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Review with novel markers facilitates precise categorization of 41 cases of diagnostically challenging, “undifferentiated small round cell tumors”. A…

2017

Abstract Background Despite extensive immunohistochemical (IHC) and molecular studies combined with morphologic findings, a group of round/ovoid cell tumors histologically similar to Ewing sarcomas (ES) but lacking EWSR1-rearrangements may remain unclassifiable. Design We retrospectively analyzed 41 Ewing-like tumors (formalin-fixed, paraffin-embedded) previously determined as negative or non-informative for EWSR1-rearrangements by FISH and/or RT-PCR. A new histopathology revision and additional IHC and molecular analyses were carried out in order to investigate whether additional IHC and/or molecular testing in combination with the morphological findings may help in reaching a definitive d…

AdultMale0301 basic medicinePathologymedicine.medical_specialtyAdolescentDesmoplastic small-round-cell tumorSarcoma EwingSclerosing rhabdomyosarcomaImmunophenotypingPathology and Forensic MedicineYoung Adult03 medical and health sciences0302 clinical medicineBiomarkers TumormedicineHumansStromal tumorChildAgedRetrospective StudiesHomeodomain ProteinsGiSTbusiness.industryNuclear ProteinsCell DifferentiationGeneral MedicineMiddle AgedPrognosismedicine.diseaseImmunohistochemistrySynovial sarcomaMolecular TypingHomeobox Protein Nkx-2.2030104 developmental biology030220 oncology & carcinogenesisSarcoma Small CellFemaleSarcomaClear-cell sarcomaRNA-Binding Protein EWSbusinessTranscription FactorsMyoepithelial TumorAnnals of Diagnostic Pathology
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Observational Prospective Natural History of Patients with Sanfilippo Syndrome Type B

2017

To evaluate the natural course of disease progression in patients with Sanfilippo syndrome type B (mucopolysaccharidosis type IIIB), identify potential end points for future therapy trials, and characterize biomarkers related to the disease.A prospective, multicenter study was conducted. Baseline, 6-month, and 12-month assessments included neurodevelopmental status (Bayley Scales of Infant Development, Third edition), adaptive status (Vineland Adaptive Behavior Scales, Second Edition), volumetric brain magnetic resonance imaging, cerebrospinal fluid heparan sulfate, and urine glycosaminoglycan (GAG) measurements.Nineteen patients aged 1.6-31.7 years were enrolled. Over 12 months, cognition,…

AdultMale0301 basic medicinePediatricsmedicine.medical_specialtyAdolescentDiseaseBayley Scales of Infant DevelopmentMucopolysaccharidosis IIIYoung Adult03 medical and health sciences0302 clinical medicineMucopolysaccharidosis IIImedicineHumansLongitudinal StudiesProspective StudiesYoung adultChildProspective cohort studyCerebrospinal FluidGlycosaminoglycansSanfilippo syndromemedicine.diagnostic_testbusiness.industryBrainInfantMagnetic resonance imagingmedicine.diseaseMagnetic Resonance ImagingVineland Adaptive Behavior Scale030104 developmental biologyNeurodevelopmental DisordersChild PreschoolPediatrics Perinatology and Child HealthDisease ProgressionFemaleHeparitin SulfatebusinessBiomarkers030217 neurology & neurosurgeryThe Journal of Pediatrics
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Selenium status during pregnancy: Influential factors and effects on neuropsychological development among Spanish infants

2017

Selenium(Se) has been positively associated with neurodevelopment in early life. However, its margin of safety is rather narrow, and few prospective studies have evaluated its potential neurotoxic effects at intermediate levels. We aimed to explore the association between maternal Se concentrations and child neuropsychological development, including the genetic effect modification of the Se metabolizing gene INMT. Study subjects were 650 mother-child pairs from the Spanish Childhood and Environment Project (INMA, 2003-2005). Infant neuropsychological development was assessed around 12 months of age by the Bayley Scales of Infant Development. Sociodemographic and dietary characteristics were…

AdultMale0301 basic medicinePediatricsmedicine.medical_specialtyEnvironmental EngineeringMultivariate analysisCognitiveNeurodevelopmentPhysiology010501 environmental sciences01 natural sciencesBayley Scales of Infant DevelopmentSelenium03 medical and health sciencesChild DevelopmentPregnancyGenotypeHumansEnvironmental ChemistryMedicinePrenatalProspective StudiesSelenium CompoundsProspective cohort studyWaste Management and DisposalChildren0105 earth and related environmental sciencesPsychomotor learningPregnancybusiness.industryInfantMethyltransferasesmedicine.diseasePollutionChild development030104 developmental biologyPrenatal Exposure Delayed EffectsMultivariate AnalysisTrace elementGestationFemalebusinessNutrient
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Notch‐1 signaling activation sustains overexpression of interleukin 33 in the epithelium of nasal polyps

2019

Abstract BACKGROUND: Alterations in the nasal epithelial barrier homeostasis and increased interleukin 33 (IL-33) expression contribute to the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP). AIMS: As Notch-1 signaling is crucial in repair processes of mucosa, the current study assessed Notch-1/Jagged-1 signaling and IL-33 in the epithelium of nasal polyps biopsies from allergic (A-CRSwNP; n = 9) and not allergic (NA-CRSwNP; n = 9) subjects by immunohistochemistry. We also assessed, in a model of nasal epithelial cells, the effects of stimulation of Notch-1 with Jagged-1 on the expression of IL-33 (by flow cytometry, immunofluorescence, and immunocytochemistry), Jagged-1 (…

AdultMale0301 basic medicineendocrine systemPhysiologyClinical BiochemistryImmunocytochemistryStimulationBiologyCell LineFlow cytometryYoung Adult03 medical and health sciencesNasal Polyps0302 clinical medicinestomatognathic systemmedicineHumansNasal polypsPhosphorylationReceptor Notch1SinusitisCyclic AMP Response Element-Binding ProteinNotch 1medicine.diagnostic_testEpithelial CellsCell BiologyMiddle AgedInterleukin-33medicine.diseaseRhinitis AllergicMolecular biologyEpitheliumUp-RegulationInterleukin 33Nasal Mucosa030104 developmental biologymedicine.anatomical_structure030220 oncology & carcinogenesisChronic DiseaseIL-33; Notch-1; chronic rhinosinusitis; nasal epithelium; nasal polypsImmunohistochemistryFemaleNOTCH-1 INTERLEUKIN 33 NASAL POLYPSJagged-1 ProteinSignal TransductionJournal of Cellular Physiology
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Genetic variations of the bitter taste receptor TAS2R38 are associated with obesity and impact on single immune traits

2015

Scope: Changes in genetic variations affecting the taste receptor, type 2, member 38 (TAS2R38) may identify the interacting mechanism leading to obesity and potential associations with proteins partaking in innate immunity, such as surfactant protein D (SPD) and mannan-binding lectin (MBL). Methods and results: We evaluated haplotypes of the bitter-taste receptor TAS2R38 in an identification sample of 210 women in different weight conditions, including anorexia nervosa and obesity. The association with SPD and MBL was tested in an independent sample picturing general population (n = 534). The relationship with obesity was validated in an extended final sample of 1319 participants. In the sa…

AdultMale0301 basic medicinemedicine.medical_specialtyAdolescentPopulation030209 endocrinology & metabolismSingle-nucleotide polymorphismBiologyMannose-Binding LectinPolymorphism Single NucleotideBody Mass IndexReceptors G-Protein-CoupledCohort StudiesYoung Adult03 medical and health sciences0302 clinical medicineInternal medicinemental disordersGenetic variationmedicineHumansObesityeducationAgedGeneticseducation.field_of_study030109 nutrition & dieteticsBody WeightSmokingHaplotypeGPR120Middle AgedPulmonary Surfactant-Associated Protein Dmedicine.diseaseObesityImmunity InnateTAS2R38EndocrinologyHaplotypesAnorexia nervosa (differential diagnoses)Case-Control StudiesTasteFemaleFood ScienceBiotechnologyMolecular Nutrition & Food Research
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Electromyographic Effect of Using Different Attentional Foci During the Front Plank Exercise

2018

Objective The aim of this cross-sectional study was to examine whether an internal focus on different core muscles during the isometric prone plank exercise increased muscle activity. Design Twenty university students performed the following five different conditions: regular prone plank (external focus) and prone plank with an internal focus on different muscles. Surface electromyography signals were recorded for the upper rectus abdominis, lower rectus abdominis, external oblique, and lumbar erector spinae. Results For the upper rectus abdominis, an internal focus on using any muscle increased activity. For the lower rectus abdominis, internal focus on any muscle except the lumbar erector…

AdultMale030506 rehabilitationmedicine.medical_specialtybridgingeducationParaspinal MusclesRectus AbdominisPhysical Therapy Sports Therapy and RehabilitationElectromyographyIsometric exercisetrunkfocusYoung Adult03 medical and health sciences0302 clinical medicinePhysical medicine and rehabilitationHealthy volunteersmind muscleHumansMedicineAttentionExercise physiologyStudentsExerciseLow backPlankCore (anatomy)medicine.diagnostic_testElectromyographybusiness.industryRehabilitationLumbosacral RegioncoreAbdominal Oblique MusclesTrunkHealthy VolunteersCross-Sectional StudiesFemalelow back0305 other medical sciencebusiness030217 neurology & neurosurgeryAmerican Journal of Physical Medicine & Rehabilitation
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