Search results for "OSP"

Modulation of Hepatitis C Virus NS5A Hyperphosphorylation by Nonstructural Proteins NS3, NS4A, and NS4B

1999

NS5A of the hepatitis C virus (HCV) is a highly phosphorylated protein involved in resistance against interferon and required most likely for replication of the viral genome. Phosphorylation of this protein is mediated by a cellular kinase(s) generating multiple proteins with different electrophoretic mobilities. In the case of the genotype 1b isolate HCV-J, in addition to the basal phosphorylated NS5A (designated pp56), a hyperphosphorylated form (pp58) was found on coexpression of NS4A (T. Kaneko, Y. Tanji, S. Satoh, M. Hijikata, S. Asabe, K. Kimura, and K. Shimotohno, Biochem. Biophys. Res. Commun. 205:320‐326, 1994). Using a comparative analysis of two full-length genomes of genotype 1b…

The closest relatives of icosahedral viruses of thermophilic bacteria are among viruses and plasmids of the halophilic archaea.

2009

We have sequenced the genome and identified the structural proteins and lipids of the novel membranecontaining, icosahedral virus P23-77 of Thermus thermophilus. P23-77 has an 17-kb circular double-stranded DNA genome, which was annotated to contain 37 putative genes. Virions were subjected to dissociation analysis, and five protein species were shown to associate with the internal viral membrane, while three were constituents of the protein capsid. Analysis of the bacteriophage genome revealed it to be evolutionarily related to another Thermus phage (IN93), archaeal Halobacterium plasmid (pHH205), a genetic element integrated into Haloarcula genome (designated here as IHP for integrated Ha…

Single amino acid substitutions in the glycoprotein B carboxy terminus influence the fusion from without property of herpes simplex virus type 1.

1995

Syncytial mutations of herpes simplex virus type 1 (HSV-1) strains ANG, ANG path, HFEM, tsB5 and HSZP cause extensive cell fusion and were mapped to the cytoplasmic domain of glycoprotein B (gB), within the syn 3 locus. These strains are so far the only ones which show the phenotype ‘fusion from without’ (FFWO): 60 min after infection with high m.o.i., cells in a tissue culture are fused without transcription and translation of the viral genome. In this report we detected, using the recombinants 27/III and K-7, that an amino acid exchange from Ala to Val at aa position 854 of gB is the main determinant for FFWO activity of strains ANG, ANG path and recombinant K-7. The transfer of this muta…

Characterization of the autoantigen La (SS-B) as a dsRNA unwinding enzyme

1997

During the analysis of the La (SS-B) autoantigen for catalytic activities an ATP-dependent double-stranded RNA unwinding activity was detected. Both native and recombinant La proteins from different species displayed this activity, which could be inhibited by monospecific anti-La antibodies. La protein was able to melt dsRNA substrates with either two 3'-overhangs or a single 3'- and a 5'-overhang. Double-stranded RNAs with two 5'-overhangs were not unwound, indicating that at least one 3'-overhang is required for unwinding. Sequence elements of the La protein that might be involved in dsRNA unwinding, such as an evolutionarily conserved putative ATP-binding motif and an element that is hom…

Electrophysiological evidence supports the role of sustained visuospatial attention in maintaining visual WM contents

2019

Recent empirical and theoretical work suggests that there is a close relationship between visual working memory (WM) and visuospatial attention. Here, we investigated whether visuospatial attention was involved in maintaining object representations in visual WM. To this end, the alpha lateralization and contralateral delay activity (CDA) were analyzed as neural markers for visuospatial attention and visual WM storage, respectively. In the single-task condition, participants performed a grating change-detection task. To probe the role of visuospatial attention in maintaining WM contents, two color squares were presented above and below the fixation point during the retention interval, which …

Visuospatiaalisten taitojen kehittyminen 7-15-vuotiailla lapsilla

2008

Positive Effects of Videogame Use on Visuospatial Competencies: The Impact of Visualization Style in Preadolescents and Adolescents

2019

Use of videogames (VGs) is almost ubiquitous in preadolescents' and adolescents' everyday life. One of the most intriguing research topics about positive effects of VG use is about the domain of visuospatial competencies. Previous research show that training with videogames enables children and adolescents to improve their scores in visuospatial tests (such as mental rotation of shapes and cubes), and that such training could overcome gender differences in these domains. Our study aimed at (1) verifying the positive effects of videogame use in the visuospatial domain both for male and female adolescents and preadolescents and (2) verifying whether the visualization style (2D or isometric 3D…

Prospektiteoria, realisoimattomat voitot ja momentum suomalaisessa aineistossa

2017

Tässä tutkielmassa tarkastellaan Grinblattin ja Hanin (2005) mukaisesti realisoimatto-mia voittoja tulevien tuottojen selittäjänä. Tämä aiheuttaa tutkijoiden julkaisussa Fama-MacBeth -regressiolla Yhdysvaltojen pörsseissä aggregaattitasolla havaittavan momentumin kaltaisen ilmiön, kun prospektiteorian ja mentaalilaskennan mukaisesti osa sijoittajista alireagoi fundamenttihintojen muutokseen aiheuttaen hintojen ennus-tettavuutta. Momentum havaitaan sekä Jegadeeshin ja Titmanin (1993) mukaisten menneiden tuottojen että realisoimattomien voittojen avulla, joista realisoimattomat voitot toimivat huomattavasti parempana selittäjänä saaden tilastollisesti merkitse-vämmän ja arvoltaan suuremman ke…

The impact of Mt. Etna volcanic emissions on the atmospheric deposition: developments and improvements during three decades of studies

2022

Volcanic emissions are an important source of gases and particles to the atmosphere. Mount Etna discharges a permanent volcanic plume consisting of water vapor and gaseous species (CO2, SO2, HCl and HF, mainly), metals, acid droplets, and solid particles. This plume interacts with meteoric water during precipitations events, so the chemical signature of the plume is impressed on the rainwater. Over the past 32 years, the impact of Etna's emissions on atmospheric deposition has been investigated in many research projects by our team. Sampling methodologies and analytical procedures have been improved over the years. The first study of the concentration of water-soluble inorganic ions in week…

A Low-Cost Smartphone Sensor-Based UV Camera for Volcanic SO2 Emission Measurements

2017

Recently, we reported on the development of low-cost ultraviolet (UV) cameras, based on the modification of sensors designed for the smartphone market. These units are built around modified Raspberry Pi cameras (PiCams; ≈USD 25), and usable system sensitivity was demonstrated in the UVA and UVB spectral regions, of relevance to a number of application areas. Here, we report on the first deployment of PiCam devices in one such field: UV remote sensing of sulphur dioxide emissions from volcanoes; such data provide important insights into magmatic processes and are applied in hazard assessments. In particular, we report on field trials on Mt. Etna, where the utility of these devices in quantif…