Search results for "OTA"
showing 10 items of 33012 documents
In Vivo RNA Labeling Using MS2
2014
The trafficking and asymmetric distribution of cytoplasmic RNA is a fundamental process during development and signaling across phyla. Plants support the intercellular trafficking of RNA molecules such as gene transcripts, small RNAs, and viral RNA genomes by targeting these RNA molecules to plasmodesmata (PD). Intercellular transport of RNA molecules through PD has fundamental implications in the cell-to-cell and systemic signaling during plant development and in the systemic spread of viral disease. Recent advances in time-lapse microscopy allow researchers to approach dynamic biological processes at the molecular level in living cells and tissues. These advances include the ability to la…
Plant genes involved in arbuscular mycorrhiza formation and functioning
2002
Knowledge about that part of the plant genome involved in the establishment and functioning of the arbuscular mycorrhizal (AM) symbiosis is important for the basic understanding of this symbiosis. It is also essential for a ‘genes to the field’ approach based on the identification and exploitation of genes that could be central to developing sustainable plant production systems in the future.
Response Mechanisms of Invertebrates to Bacillus thuringiensis and Its Pesticidal Proteins
2021
SUMMARY Extensive use of chemical insecticides adversely affects both environment and human health. One of the most popular biological pest control alternatives is bioinsecticides based on Bacillus thuringiensis. This entomopathogenic bacterium produces different protein types which are toxic to several insect, mite, and nematode species. Currently, insecticidal proteins belonging to the Cry and Vip3 groups are widely used to control insect pests both in formulated sprays and in transgenic crops. However, the benefits of B. thuringiensis-based products are threatened by insect resistance evolution. Numerous studies have highlighted that mutations in genes coding for surrogate receptors are …
Intracellular compartmentation and plant cell signalling
1997
Compartmentation is an essential feature of eukaryotic cells, and is crucial for the regulation of cell metabolism. Recent progress has significantly improved the understanding of signal transduction pathways in plants, including the activation of light-signalling networks and the tightly controlled generation of the calcium message. Cell compartmentation is important for the regulation and proliferation of these signalling processes.
Effects of fusaric acid on cells from tomato cultivars resistant or susceptible toFusarium oxysporum f. sp.Lycopersici
1996
Cell suspension cultures were set up from two tomato cultivars, one resistant, (‘Rio grande’) and one susceptible (‘63.5’) toFusarium oxysporum f. sp.lycopersici. Growth rates of the two cell cultures were comparable. Toxicity of fusaric acid, expressed as the fresh weight loss, was analyzed: It was significant in both cases after 10 h, but toxicity was twice as high for ‘63.5’ suspension cells. In the same way, electrolyte leakage caused by fusaric acid was three times more important for ‘63.5’ suspension cells. Moreover, fusaric acid treatment resulted in an acidification of the extracellular medium for ‘63.5’ suspension cells (0.4 pH unit), whereas an alkalization was observed for ‘Rio g…
PERMANENT GENETIC RESOURCES: Polymorphic microsatellite loci and interspecific cross-amplification in the parasitoid wasps Megastigmus stigmatizans a…
2008
We isolated and characterized 19 polymorphic microsatellite loci in the congeneric parasitoid wasps Megastigmus stigmatizans and Megastigmus dorsalis associated with cynipid oak galls. Loci isolated from species-specific libraries showed extensive cross-amplification, resulting in a total of 15 polymorphic loci for M. stigmatizans and 13 for M. dorsalis.
‘Communication breakdown’: the evolution of signal unreliability and deception
2014
For a signalling system to be stable, signals must confer net fitness benefits to senders and receivers, which means that some aspect of their design must correlate with a quality that receivers benefit from knowing about. However, examples abound where this correlation is complicated by phenomena commonly referred to as deception and/or signal unreliability. We argue here that unreliability and deception are notions marred with conceptual ambiguities, often used as equivalent or as catch-all terms for qualitatively different processes. Signal unreliability refers to a pattern of design–information dissociation that can arise through different processes, some deceptive and some not, with di…
Time course analysis of the brain transcriptome during transitions between brood care and reproduction in the clonal raider ant
2017
AbstractDivision of labor between reproductive queens and non-reproductive workers that perform brood care is the hallmark of insect societies. However, the molecular basis of this fundamental dichotomy remains poorly understood, in part because the caste of an individual cannot typically be experimentally manipulated at the adult stage. Here we take advantage of the unique biology of the clonal raider ant,Ooceraea biroi, where reproduction and brood care behavior can be experimentally manipulated in adults. To study the molecular regulation of reproduction and brood care, we induced transitions between both states, and monitored brain gene expression at multiple time points. We found that …
Antioxidant Phenolic Compounds in Salvia officinalis L. and Salvia sclarea L.
2018
Abstract The differences in the chemical composition of the phenolic compounds of Salvia officinalis versus Salvia sclarea growing in different habitats, were studied. First, the optimal solvent - methanol - for ultrasonic extraction of phenolic compounds from these plants was chosen experimentally. Total phenolic content and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays were determined spectrophotometrically. Total phenolic content was in the range of 63.9 to 134.4 mg GAE/g of plant depending on the studied species. The highest antiradical activity was displayed by the methanol fractions of S. sclarea varieties (83 and 67%). HPLC-DAD analysis of extracts was done in order to identify the pre…
Co-Designing Social Simulation Models For Policy Advise: Lessons Learned From the INFSO-SKIN Study
2019
This paper describes a participatory approach to co-designing social simulation models with policymakers using a case study of modeling European Commission policy. Managing the collaboration of a wide range of individuals or organizations is challenging but increasingly important as policy making becomes more complex. A framework for a co-design process based on a participatory approach is proposed. The framework suggests that the collaborative design should go through the following phases: Identifying user questions, data provision, model discussion for validation, visualization of results and discussing scope and limitations with stakeholders. Key findings are that the co-design process r…