Search results for "Oligomycin"

Solubilization of an oligomycin-sensitive ATPase complex fromRhodospirillum rubrumchromatophores and its inhibition by various antibiotics

1978

Purification of ATP synthase from beef heart mitochondria (FoF1) and co-reconstitution with monomeric bacteriorhodopsin into liposomes capable of lig…

1993

ATP synthase was isolated from beef heart mitochondria by extraction with N,N-bis-(3-D-gluconamidopropyl)deoxycholamide or by traditional cholate extraction. The enzyme was purified subsequently by ion-exchange and gel-permeation chromatographies in the presence of glycerol and the protease inhibitor diisopropylfluorophosphate. The ATP synthase consisted of 12–14 subunits and contained three tightly bound nucleotides. The co-reconstitution of crude or purified ATP synthase with monomeric bacteriorhodopsin by the method of detergent incubation of liposomes yielded proteoliposomes capable of light-driven ATP synthesis, as detected with a luciferase system for at least 30 min. The reaction was…