Search results for "Optical transfer function"
showing 10 items of 53 documents
Recent Advances in 3D Structured Illumination Microscopy
2018
In structured illumination microscopy (SIM) the sample under investigation is illuminated using a structured illumination (SI) pattern. This SI pattern encodes high spatial frequencies of fine features within the sample, which usually are not transferred by the conventional three-dimensional (3D) optical transfer function (OTF) of the imaging system and fills the missing cone of frequencies in the OTF for better discrimination of the out-of-focus light. Thereby, SIM provides super-resolution (SR) performance beyond the diffraction limit and optical-sectioning (OS) capability with the use of data post-processing approaches. 3D structured patterns that include lateral and axial variations in …
Investigating the performance of reconstruction methods used in structured illumination microscopy as a function of the illumination pattern's modula…
2016
Surpassing the resolution of optical microscopy defined by the Abbe diffraction limit, while simultaneously achieving optical sectioning, is a challenging problem particularly for live cell imaging of thick samples. Among a few developing techniques, structured illumination microscopy (SIM) addresses this challenge by imposing higher frequency information into the observable frequency band confined by the optical transfer function (OTF) of a conventional microscope either doubling the spatial resolution or filling the missing cone based on the spatial frequency of the pattern when the patterned illumination is two-dimensional. Standard reconstruction methods for SIM decompose the low and hi…
Fractal-structured multifocal intraocular lens
2017
[EN] In this work, we present a new concept of IOL design inspired by the demonstrated properties of reduced chromatic aberration and extended depth of focus of Fractal zone plates. A detailed description of a proof of concept IOL is provided. The result was numerically characterized, and fabricated by lathe turning. The prototype was tested in vitro using dedicated optical system and software. The theoretical Point Spread Function along the optical axis, computed for several wavelengths, showed that for each wavelength, the IOL produces two main foci surrounded by numerous secondary foci that partially overlap each other for different wavelengths. The result is that both, the near focus an…
White-light imaging with fractal zone plates
2007
We report the achievement of the first images to our knowledge obtained with a fractal zone plates (FraZPs). FraZPs are diffractive lenses characterized by the fractal structure of their foci. This property predicts an improved performance of FraZPs as image forming devices with an extended depth of field and predicts a reduced chromatic aberration under white-light illumination. These theoretical predictions are confirmed experimentally in this work. We show that the polychromatic modulation transfer function of a FraZP affected by defocus is about two times better than one corresponding to a Fresnel zone plate.
Accommodation in human eye models: a comparison between the optical designs of Navarro, Arizona and Liou-Brennan.
2017
Aim To simulate and compare accommodation in accommodative and non-accommodative human eye models. Methods Ray tracing and optical design program was used. Three eye models were designed and studied: the Navarro, the Arizona and the Liou-Brennan. In order to make the Navarro and Liou-Brennan models to accommodate, specific geometric parameters of the models were altered with values that were chosen from the literature. For the Arizona model, its' mathematical functions for accommodation were used for the same accommodative demands. The simulation included four distances of accommodation for each model: at infinity, 3, 1 and 0.5 m.The results were diffraction images of a "letter F" for graph…
Transverse resolution improvement using rotating-grating time-multiplexing approach
2008
The ability to improve the limited resolving power of optical imaging systems while approaching the theoretical diffraction limit has been an attractive discipline with growing interest over the last years due to its benefits in many applied optics systems. This paper presents a new approach to achieve transverse superresolution in far-field imaging systems, with direct application in both digital microscopy and digital holographic microscopy. Theoretical analysis and computer simulations show the validity of the presented approach.
Performance analysis of optical imaging systems based on the fractional fourier transform
1998
Some image quality parameters, such as the Strehl ratio and the optical transfer function, are analysed in the generalized phase-space, or x-p domain, of the fractional Fourier transform associated with a modified one-dimensional pupil function. Some experimental results together with computer simulations are performed which illustrate the tolerance to defocus of different apertures.
Optical quality comparison among different Boston contact lens materials.
2014
BACKGROUND The aim was to assess the optical quality of four Boston contact lens materials with an optical device based on Schlieren interferometry. METHODS The NIMO TR1504 (Lambda-X, Nivelles, Belgium) was used to measure higher-order aberrations and their corresponding root mean square values of four different rigid gas permeable contact lenses made from four different Boston materials: EO, ES, XO and XO2 . For each lens, 30 measurements were performed with two optical apertures: 3.0 mm and 6.0 mm. The modulation transfer function, point spread function, Strehl ratio and a simulation of the image provided by the lens were computed from the Zernike coefficients measured up to the fourth or…
Optical transfer function engineering for a tunable 3D structured illumination microscope
2019
Two important features of three-dimensional structured illumination microscopy (3D-SIM) are its optical sectioning (OS) and super-resolution (SR) capabilities. Previous works on 3D-SIM systems show that these features are coupled. We demonstrate that a 3D-SIM system using a Fresnel biprism illuminated by multiple linear incoherent sources provides a structured illumination pattern whose lateral and axial modulation frequencies can be tuned separately. Therefore, the compact support of the synthetic optical transfer function (OTF) can be engineered to achieve the highest OS and SR capabilities for a particular imaging application. Theoretical performance of our engineered system based on the…
Imaging quality of multifocal intraocular lenses: automated assessment setup
2012
[EN] Purpose: A new technique for the assessment of the optical quality of multifocal intraocular lenses (MIOLs) under monochromatic and polychromatic illumination is presented. Methods: The system provides, in a totally automated procedure, the modulation transfer function (MTF) of the lens under test for different axial positions of the object. The artificial eye admits different artificial corneas, to optimise the axial resolution in the sampling of the MTF of the MIOL under test, and different pupils, to test the dependence of the optical performance of the MIOL on the eye pupil diameter. Results: The performance and sensitivity of the apparatus is tested with different commercial MIOLs…