Search results for "Oration"
showing 10 items of 2042 documents
Una pequeña lámina de plomo con inscripción ibérica de paleografía arcaica del Pico de los Ajos (Yátova, València)
2021
espanolEn este trabajo editamos una pequena lamina de plomo hallada en el Pico de los Ajos (Yatova) en la campana de 2018. La lamina aparecio plegada y contiene una inscripcion iberica de una sola linea en cada cara que muy probablemente configure un solo texto completo. Sus caracteristicas formales son poco comunes, siendo el paralelo mas cercano el de las laminas votivas de El Amarejo. La presencia del elemento kutuŕ, frecuente en las inscripciones votivas rupestres, tambien apuntaria a una interpretacion religiosa. Desde el punto de vista paleografico, cabe senalar que se trata de un texto explicitamente dual, ya que conviven en el las dos variantes de te y ŕ. A pesar de que el contexto …
Aspetti tecnologici per il riuso: contributi al dibattito
2016
Qui il taglio della trattazione sul riuso della Centrale Enel Tifeo è legato alle questioni tecnologiche sia pure in un’accezione ampia che declina sia le tecniche ambientali che le tecnologie connesse agli edifici e alle costruzioni. Una lettura disciplinare, certamente parziale e limitata, che non vuole mancare però di individuare fattori comuni e temi di dibattito di più ampio respiro. Si tenta questa analisi con una struttura di tre paragrafi, parzialmente correlati a scale di problemi: il contesto e il lotto, quale area di dominio; l’involucro, soprattutto considerato per l’edificio dei turbo-alternatori; l’ambiente indoor e gli spazi interni in edifici dalla spazialità estremamente pa…
REHABILITATION, ENHANCEMENT AND PROMOTION OF ART NOUVEAU BUILDINGS
2016
Since ancient times every era has produced a particular architectural heritage with different materials and with the use of different construction. This study aims to make a contribution in terms of knowledge and the architectural heritage of the 20th century, recovery, and in particular those representing the Sicilian architecture known as Liberty architecture. The study explores the relationship between architecture, materials, construction techniques and the building restoration and enhancement problems. The main purpose of this research is on the one hand the analysis and technological knowledge of the architectural heritage and the other to study the types of …
C57BL/6-specific conditions for efficient in utero electroporation of the central nervous system.
2014
Abstract Background In utero electroporation is a fast an efficient tool to specifically address gene expression in the murine central nervous system. This technique was originally established in ICR/CD-1 outbred mice. Neuroanatomical differences between the different mouse strains and variations in gestation length require the optimization of the conditions for each strain to avoid severe complications. Furthermore the relevant position information is currently only scarcely standardized and not always easy to transfer to C57BL/6 mice. New method In this study we present an improved method for in utero electroporation of C57BL/6 including a detailed atlas that allows for specific and effic…
T-cell receptor transfer into human T cells with ecotropic retroviral vectors
2014
Adoptive T-cell transfer for cancer immunotherapy requires genetic modification of T cells with recombinant T-cell receptors (TCRs). Amphotropic retroviral vectors (RVs) used for TCR transduction for this purpose are considered safe in principle. Despite this, TCR-coding and packaging vectors could theoretically recombine to produce replication competent vectors (RCVs), and transduced T-cell preparations must be proven free of RCV. To eliminate the need for RCV testing, we transduced human T cells with ecotropic RVs so potential RCV would be non-infectious for human cells. We show that transfection of synthetic messenger RNA encoding murine cationic amino-acid transporter 1 (mCAT-1), the re…
A novel plasmid DNA electroporation method allows transfection of murine DC.
2007
Under steady state conditions dendritic cells (DC) exert tolerogenic function, but acquire potent immunogenic function due to strong upregulation of costimulatory molecules and proinflammatory cytokines. In numerous studies the potential of modified DC to induce tolerance or immune reactions towards a distinct antigen has been demonstrated. However, DC are refractory to transfection with plasmid DNA by non-viral methods. In this study we have tested the suitability of a newly developed electroporation device to transfect immature murine bone-marrow derived DC (BM-DC). Transfected BM-DC expressed reporter molecules at considerable extent which renders this method suitable to perform all kind…
Gaining or losing PhDs: What are the effects on firms' linkages with universities?
2023
PhD graduates can help companies transfer knowledge from universities to firms. Scholars have analysed the determinants of PhD recruitment by firms and its effects on their innovation activities. However, little is known about what happens when a firm loses employees with PhDs. The aim of this paper is to compare the effects on the relationships of firms with universities when these firms lose PhDs versus when they hire PhDs to work in R&D. These effects may be symmetrical or non-symmetrical depending on the abilities of firms to retain the connections and knowledge acquired by hiring PhDs. We consider four types of relationships: collaboration with universities, universities as a source of…
La Constitution, les Français et les opinions dissidentes : une histoire de piano et de tabouret
2010
International audience
Camillo Boito e la sua precoce teorizzazione del restauro architettonico
2017
Camillo Boito è stato considerato, a partire dal primo Novecento, come «ispiratore della moderna teoria del restauro» o come «ideatore della concezione intermedia tra le posizioni di Viollet-le-Duc e Ruskin». Il contributo intende fornire nuovi elementi per inquadrare più nitidamente il reale apporto di Boito alla definizione del restauro come disciplina. From the beginning of the XX century, Camillo Boito was considered the "inspirer of the modern restoration theory" or the "creator of the intermediate position between the ones of Viollet-le-Duc and John Ruskin". This contribution aims to provide new elements to frame Boito's real contribution to the definition of restoration as a discipli…
CD83+ human dendritic cells transfected with tumor peptide cDNA by electroporation induce specific T-cell responses: A potential tool for gene immuno…
2000
Dendritic cells (DC) are the most potent immunostimulatory cells, with the capacity to induce primary T-cell responses. Functional autologous DC can be generated from fetal calf serum-free peripheral blood mononuclear cells in the presence of interleukin-4 and granulocyte-macrophage colony-stimulating factor and are stimulated with a defined cytokine cocktail for terminal maturation. We were able to establish a nonviral transfection protocol for these DC by electroporation. Using enhanced green fluorescent protein as a reporter gene, we achieved transfection efficiencies of up to 10%. FACScan analyses revealed a stable phenotype, and the expression of major histocompatibility complex class …