Search results for "Organisms"

showing 10 items of 726 documents

Home-based HIV testing: Using different strategies among transgender women in Argentina.

2019

Background In Argentina, HIV prevalence among transgender women (TGW) has been reported at 34%. The stigma is one of the most important factors limiting their access to healthcare services. The aims of this study were to compare different HIV testing methodologies, to determine the factors associated with HIV diagnosis and to determine the feasibility of a home-based HIV testing service for TGW. Methods A multidisciplinary team performed home-based HIV testing interventions in four cities of Argentina. Participants self-identified as TGW, older than 14 years and with a negative or unknown HIV status. Blood samples were screened by two rapid tests (RT), one based on antibodies (Determine™ HI…

RNA virusesMultivariate analysisEpidemiologyPsychological interventionSocial SciencesHIV InfectionsLogistic regressionPathology and Laboratory MedicineTransgender womenGeographical locations0302 clinical medicineImmunodeficiency VirusesSociologyMedicine and Health Sciences030212 general & internal medicineYoung adultEnzyme-Linked Immunoassayseducation.field_of_studyMultidisciplinarySchoolsQRvirus diseasesHIV diagnosis and managementMedical MicrobiologyHIV epidemiologyViral PathogensVirusesMedicineInfectious diseasesFemalePathogens0305 other medical scienceViral loadResearch ArticleAdultmedicine.medical_specialtySciencePopulationArgentinaSexually Transmitted DiseasesHiv testingViral diseasesResearch and Analysis MethodsTransgender PersonsMicrobiologyEducation03 medical and health sciencesYoung AdultInternal medicineRetrovirusesmedicineHumanseducationImmunoassaysMicrobial Pathogens030505 public healthbusiness.industryLentivirusOrganismsBiology and Life SciencesHIVSouth AmericaDiagnostic medicineLogistic ModelsMultivariate AnalysisImmunologic TechniquesFeasibility StudiesReagent Kits DiagnosticPeople and placesbusinessPloS one
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Large-scale analysis of SARS-CoV-2 spike-glycoprotein mutants demonstrates the need for continuous screening of virus isolates

2021

Due to the widespread of the COVID-19 pandemic, the SARS-CoV-2 genome is evolving in diverse human populations. Several studies already reported different strains and an increase in the mutation rate. Particularly, mutations in SARS-CoV-2 spike-glycoprotein are of great interest as it mediates infection in human and recently approved mRNA vaccines are designed to induce immune responses against it. We analyzed 1,036,030 SARS-CoV-2 genome assemblies and 30,806 NGS datasets from GISAID and European Nucleotide Archive (ENA) focusing on non-synonymous mutations in the spike protein. Only around 2.5% of the samples contained the wild-type spike protein with no variation from the reference. Among…

RNA virusesMutation rateCoronavirusesEpidemiologyMolecular biologyT-LymphocytesMutantGene Identification and Analysismedicine.disease_causeGenomeWhite Blood CellsDatabase and Informatics MethodsSequencing techniquesMutation RateAnimal CellsDNA sequencingPathology and laboratory medicineGeneticsMutationMultidisciplinaryT CellsMicrobial MutationQRHigh-Throughput Nucleotide SequencingGenomicsMedical microbiologyVirusesSpike Glycoprotein CoronavirusMedicineSARS CoV 2PathogensCellular TypesTranscriptome AnalysisSequence AnalysisResearch ArticleNext-Generation SequencingSARS coronavirusBioinformaticsImmune CellsScienceImmunologyProtein domainSequence alignmentGenomicsGenome ViralBiologyMicrobiologyAntibodiesDNA sequencingProtein DomainsGeneticsmedicineHumansMutation DetectionPandemicsMedicine and health sciencesBlood CellsBiology and life sciencesSARS-CoV-2OrganismsViral pathogensComputational BiologyCOVID-19Cell BiologyGenome AnalysisMicrobial pathogensResearch and analysis methodsMolecular biology techniquesMutationSequence AlignmentPLOS ONE
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Anti-prothrombin autoantibodies enriched after infection with SARS-CoV-2 and influenced by strength of antibody response against SARS-CoV-2 proteins

2021

Antiphospholipid antibodies (aPL), assumed to cause antiphospholipid syndrome (APS), are notorious for their heterogeneity in targeting phospholipids and phospholipid-binding proteins. The persistent presence of Lupus anticoagulant and/or aPL against cardiolipin and/or β2-glycoprotein I have been shown to be independent risk factors for vascular thrombosis and pregnancy morbidity in APS. aPL production is thought to be triggered by–among other factors–viral infections, though infection-associated aPL have mostly been considered non-pathogenic. Recently, the potential pathogenicity of infection-associated aPL has gained momentum since an increasing number of patients infected with Severe Acu…

RNA virusesPulmonologyCoronavirusesPhysiology2405 ParasitologyAntibody Response030204 cardiovascular system & hematologyBiochemistrychemistry.chemical_compoundMedical Conditions0302 clinical medicineimmune system diseasesImmune PhysiologyMedicine and Health SciencesCardiolipinMedicineBiology (General)skin and connective tissue diseasesImmune ResponsePathology and laboratory medicineCOVIDVirus Testing0303 health sciencesLupus anticoagulantImmune System Proteinsbiologymedicine.diagnostic_test2404 MicrobiologyProteasesMedical microbiologyEnzymes3. Good healthInfectious DiseasesCoagulationVirusesSARS CoV 2PathogensAntibodyResearch ArticleSARS coronavirusQH301-705.5Immunology10208 Institute of Neuropathology610 Medicine & healthSARS-CoV-2; Respiratory infections; Virus testing; serine proteases; Antibody response; Autoantibodies; Blood plasma; ImmunoassaysResearch and Analysis MethodsMicrobiologyAntibodiesRespiratory Disorders03 medical and health sciences1311 GeneticsDiagnostic MedicineAntiphospholipid syndromeVirology1312 Molecular BiologyGeneticsImmunoassaysneoplasmsMolecular BiologyAutoantibodies030304 developmental biology030203 arthritis & rheumatology2403 ImmunologyPregnancyBiology and life sciencesbusiness.industryOrganismsViral pathogensAutoantibodyProteinsRC581-607medicine.diseaseMicrobial pathogenschemistry19ImmunoassayRespiratory InfectionsImmunology2406 VirologyEnzymologyImmunologic Techniquesbiology.protein570 Life sciences; biologyParasitologyImmunologic diseases. AllergySerine ProteasesbusinessPLOS Pathogens
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Risk factors for gastroenteritis associated with canal swimming in two cities in the Netherlands during the summer of 2015: A prospective study

2017

Urban canal swimming events are popular in the Netherlands. In 2015, two city canal swimming events took place, in Utrecht (Utrecht Singel Swim, USS) and in Amsterdam (Amsterdam City Swim, ACS). This prospective study characterizes the health risks associated with swimming in urban waters. Online questionnaires were sent to 160 (USS) and 2,692 (ACS) participants, with relatives of participants who did not swim completing the questionnaire as a control. Swimming water specimens and stool specimens of diarrheic participants in the ACS group were analysed. A total of 49% of USS and 51% of ACS swimmers returned their questionnaires. Nine percent of USS swimmers and 4% of non-swimmers reported g…

RNA virusesQuestionnaires0301 basic medicineMalePediatricsPhysiologyWater contaminationlcsh:MedicineFresh WaterPathology and Laboratory Medicinemedicine.disease_causeDisease OutbreaksFecesRisk FactorsSurveys and QuestionnairesWater QualityEpidemiologyMedicine and Health SciencesProspective StudiesProspective cohort studylcsh:ScienceCaliciviridae InfectionsNetherlandsMultidisciplinarySewageEnterobacteriaceae InfectionsMiddle AgedBacterial PathogensGastroenteritisMedical MicrobiologyResearch DesignViral PathogensVirusesEngineering and TechnologyFemalePathogensAnatomyWater MicrobiologyResearch ArticleAdultmedicine.medical_specialtyEnvironmental EngineeringAdolescent030106 microbiologyResearch and Analysis MethodsMicrobiologyCaliciviruses03 medical and health sciencesYoung AdultEnterobacteriaceaeSurface WaterEnvironmental healthmedicineHumansMicrobial PathogensSwimmingSurvey ResearchBacteriaBiological Locomotionbusiness.industryNorovirusWater Pollutionlcsh:ROrganismsBiology and Life SciencesOutbreakGastrointestinal TractEarth SciencesNoroviruslcsh:QHydrologybusinessDigestive SystemEnterococcus
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Unraveling the role of the secretor antigen in human rotavirus attachment to histo-blood group antigens

2019

25 Páginas, 7 figuras, 2 tablas

RNA virusesRotavirusViral DiseasesPhysiologyViral Nonstructural ProteinsPathology and Laboratory MedicineCrystallography X-Raymedicine.disease_causeBiochemistryBinding AnalysisReovirusesImmune PhysiologyRotavirusMedicine and Health SciencesChemical PrecipitationBiology (General)Antigens ViralGastroenterologiachemistry.chemical_classification0303 health sciencesImmune System ProteinsCrystallographyMolecular StructurebiologyPhysics030302 biochemistry & molecular biologyChemical ReactionsRNA-Binding ProteinsCondensed Matter PhysicsLigand (biochemistry)Amino acidChemistryInfectious DiseasesMedical MicrobiologyViral PathogensVirusesPhysical SciencesCrystal StructurePathogensCrystallizationResearch ArticleChemical ElementsGlycanQH301-705.5Virus RNAViral proteinImmunologyResearch and Analysis MethodsMicrobiologyABO Blood-Group SystemCell Line03 medical and health sciencesAntigenVirologyGeneticsmedicineSolid State PhysicsHumansAntigensBinding siteMicrobial PathogensMolecular BiologyRotavirus InfectionChemical Characterization030304 developmental biologyChemical PhysicsBinding SitesBiology and life sciencesMutagenesisOrganismsProteinsRC581-607Molecular biologyCarbonchemistrybiology.proteinCapsid ProteinsParasitologyImmunologic diseases. AllergyPLOS Pathogens
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Investigation of superspreading COVID-19 outbreak events in meat and poultry processing plants in Germany: A cross-sectional study.

2020

Since May 2020, several COVID-19 outbreaks have occurred in the German meat industry despite various protective measures, and temperature and ventilation conditions were considered as possible high-risk factors. This cross-sectional study examined meat and poultry plants to examine possible risk factors. Companies completed a self-administered questionnaire on the work environment and protective measures taken to prevent SARS-CoV-2 infection. Multivariable logistic regression analysis adjusted for the possibility to distance at least 1.5 meters, break rules, and employment status was performed to identify risk factors associated with COVID-19 cases. Twenty-two meat and poultry plants with 1…

RNA virusesViral DiseasesMeat packing industryCross-sectional studyEconomicsCoronavirusesSocial SciencesLogistic regressionPoultrylaw.inventionDisease OutbreaksMedical ConditionslawRisk FactorsAnimal ProductsGermanyMedicine and Health SciencesMedicineFood IndustryPublic and Occupational HealthWorkplacePathology and laboratory medicineVirus TestingMultidisciplinaryPhysicsQRTemperatureClassical MechanicsEukaryotaAgriculturePoultry farmingMedical microbiologyDynamicsMeat ProductsInfectious DiseasesAir FlowVentilation (architecture)VirusesPhysical SciencesVertebratesMedicineSARS CoV 2PathogensResearch ArticleEmploymentPhysics - Physics and SocietyMeatCoronavirus disease 2019 (COVID-19)SARS coronavirusScienceFOS: Physical sciencesPhysics and Society (physics.soc-ph)MicrobiologyBirdsAerodynamicsDiagnostic MedicineEnvironmental healthHumansAnimalsNutritionbusiness.industrySARS-CoV-2OrganismsViral pathogensOutbreakCOVID-19Biology and Life SciencesCovid 19Odds ratioPhysics - Medical PhysicsVentilationDietMicrobial pathogensCross-Sectional StudiesFoodLabor EconomicsAmniotesMedical Physics (physics.med-ph)businessZoologyPloS one
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Evolutionary plasticity of SH3 domain binding by Nef proteins of the HIV-1/SIVcpz lentiviral lineage

2021

The accessory protein Nef of human and simian immunodeficiency viruses (HIV and SIV) is an important pathogenicity factor known to interact with cellular protein kinases and other signaling proteins. A canonical SH3 domain binding motif in Nef is required for most of these interactions. For example, HIV-1 Nef activates the tyrosine kinase Hck by tightly binding to its SH3 domain. An archetypal contact between a negatively charged SH3 residue and a highly conserved arginine in Nef (Arg77) plays a key role here. Combining structural analyses with functional assays, we here show that Nef proteins have also developed a distinct structural strategy—termed the "R-clamp”—that favors the formation …

RNA virusesviruksetvirusesSimian Acquired Immunodeficiency SyndromeHIV InfectionsPathology and Laboratory MedicineSH3 domainWhite Blood CellsImmunodeficiency VirusesAnimal CellsMedicine and Health SciencesBiology (General)MammalsGenetics11832 Microbiology and virology0303 health sciencesKinase030302 biochemistry & molecular biologyEukaryotavirus diseasesTransfection3. Good healthSIVMedical MicrobiologyViral PathogensViral evolutionVirusesVertebratesProto-Oncogene Proteins c-hckApesSimian Immunodeficiency VirusPathogensCellular TypesTyrosine kinaseResearch ArticlePrimateskinaasitEvolutionary ImmunologyLineage (genetic)QH301-705.5Immune CellsImmunologyevoluutioBiologyTransfectionResearch and Analysis MethodsHIV-tartuntaMicrobiologyViral EvolutionEvolution Molecularsrc Homology Domains03 medical and health sciencesVirologyRetrovirusesGeneticsAnimalsHumansLuciferaseAmino Acid Sequencenef Gene Products Human Immunodeficiency VirusChimpanzeesMolecular Biology TechniquesMicrobial PathogensMolecular Biology030304 developmental biologyEvolutionary BiologyBlood CellsSequence Homology Amino AcidMacrophagesLentivirusOrganismsBiology and Life SciencesHIVCell BiologyRC581-607Organismal Evolution3121 General medicine internal medicine and other clinical medicineMicrobial EvolutionAmniotesHIV-1ParasitologySalt bridgeproteiinitImmunologic diseases. AllergyZoology
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Bacterial Diseases of Bioenergy Woody Plants in Ukraine

2023

In this study, the characterization of several bacterial diseases affecting silver birch (Betula pendula Roth.), common ash (Fraxinus excelsior L.), white poplar (Populus alba L.), and white willow (Salix alba L.) in Ukraine were described. The typical symptoms, features of pathogenesis, and characteristics of the causative agents of the most common bacterial diseases of these tree species were shown. The following types of bacterioses were noted to be especially dangerous, namely, bacterial wetwood, fire blight, bacterial canker, and tuberculosis. Bacterial necrosis of the bark was a less dangerous disease. At the same time, all of the listed types of bacterioses were registered within the…

Renewable Energy Sustainability and the EnvironmentetiologyGeography Planning and Developmentbacterial plant diseasessymptomsBuilding and ConstructionManagement Monitoring Policy and LawmicroorganismsSustainability
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Chemical composition and particle size influence the toxicity of nanoscale plastic debris and their co-occurring benzo(α)pyrene in the model aquatic …

2021

Little is known about how particle chemical composition and size might influence the toxicity of nanoscale plastic debris (NPD) and their co-occurring chemicals. Herein, we investigate the toxicity of 3 × 1010 particles/L polyethylene (PE, 50 nm), polypropylene (PP, 50 nm), polystyrene (PS, 200 and 600 nm), and polyvinyl chloride (PVC, 200 nm) NPD and their co-occurring benzo(a)pyrene (BaP) to Daphnia magna and Danio rerio. During the 21 days of exposure to PE 50 nm and PS 200 nm, the number of broods produced by D. magna decreased compared to other treatments. Exposure to BaP alone did not produce any effects on the reproduction of the daphnids, however, the mixture of BaP with PS (200 or …

Reproductive toxicityAquatic OrganismsMaterials Science (miscellaneous)Benzo(a)pyreneAnimalspolyeteeniympäristömyrkytEcotoxicityseeprakalaParticle SizePolyvinyl ChlorideSafety Risk Reliability and QualityZebrafishpolyvinyylikloridiPolyvinyl chloridePublic Health Environmental and Occupational HealthmikroroskatekotoksikologiaDaphniamuoviPolyethylenevesikirputEcotoxicity; Nanoplastics; Polyethylene; Polypropylene; Polyvinyl chloride; Reproductive toxicityalkionkehitysNanoplasticsPolypropylenePlasticsSafety ResearchWater Pollutants ChemicalNanoImpact
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Low intensity vs. self-guided Internet-delivered psychotherapy for major depression: A multicenter, controlled, randomized study

2013

Abstract Background Major depression will become the second most important cause of disability in 2020. Computerized cognitive-behaviour therapy could be an efficacious and cost-effective option for its treatment. No studies on cost-effectiveness of low intensity vs self-guided psychotherapy has been carried out. The aim of this study is to assess the efficacy of low intensity vs self-guided psychotherapy for major depression in the Spanish health system. Methods The study is made up of 3 phases: 1.- Development of a computerized cognitive-behaviour therapy for depression tailored to Spanish health system. 2.- Multicenter controlled, randomized study: A sample (N=450 patients) with mild/mod…

Research designMalemedicine.medical_treatmentCost-Benefit Analysis:Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Methods::Research Design [Medical Subject Headings]:Named Groups::Persons::Age Groups::Adult::Middle Aged [Medical Subject Headings]law.invention:Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings]Study ProtocolRandomized controlled trialQuality of lifeClinical Protocols:Psychiatry and Psychology::Mental Disorders::Mood Disorders::Depressive Disorder::Depressive Disorder Major [Medical Subject Headings]law:Analytical Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Clinical Protocols [Medical Subject Headings]Tratamiento asistido por ordenadorDepression (differential diagnoses)DepressionMiddle Aged:Psychiatry and Psychology::Behavioral Disciplines and Activities::Psychotherapy::Behavior Therapy::Cognitive Therapy [Medical Subject Headings]Psychiatry and Mental healthTreatment OutcomeResearch DesignRandomized controlled trial:Information Science::Information Science::Computing Methodologies::Computer Systems::Computer Communication Networks::Internet [Medical Subject Headings]FemaleAnálisis Costo-BeneficioPsychologyAdultmedicine.medical_specialtyPsychotherapist:Check Tags::Male [Medical Subject Headings]:Analytical Diagnostic and Therapeutic Techniques and Equipment::Therapeutics::Therapy Computer-Assisted [Medical Subject Headings]:Health Care::Health Care Economics and Organizations::Economics::Costs and Cost Analysis::Cost-Benefit Analysis [Medical Subject Headings]Trastorno depresivo mayor:Named Groups::Persons::Age Groups::Adult [Medical Subject Headings]medicineHumansPsychiatryComputer-delivered psychotherapy:Analytical Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Prognosis::Treatment Outcome [Medical Subject Headings]Depressive Disorder MajorInternetIntention-to-treat analysisCognitive Behavioral TherapyBeck Depression InventoryClinical trial:Check Tags::Female [Medical Subject Headings]Diseño de la investigaciónTherapy Computer-AssistedProtocolos clínicosCognitive therapyResultado del tratamientoTerapia cognitiva
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