Search results for "Original article"

showing 10 items of 1187 documents

L-asparaginase inhibits invasive and angiogenic activity and induces autophagy in ovarian cancer

2012

Recent work identified L-asparaginase (L-ASP) as a putative therapeutic target for ovarian cancer. We suggest that L-ASP, a dysregulator of glycosylation, would interrupt the local microenvironment, affecting the ovarian cancer cell-endothelial cell interaction and thus angiogenesis without cytotoxic effects. Ovarian cancer cell lines and human microvascular endothelial cells (HMVEC) were exposed to L-ASP at physiologically attainable concentrations and subjected to analyses of endothelial tube formation, invasion, adhesion and the assessment of sialylated proteins involved in matrix-associated and heterotypic cell adhesion. Marked reduction in HMVEC tube formation in vitro, HMVEC and ovari…

Cell typeautophagyGlycosylationAngiogenesisCellOligosaccharidesAngiogenesis InhibitorsBiologyL-asparaginase; ovarian cancer; angiogenesisCell-Matrix JunctionsangiogenesisSettore BIO/13 - Biologia ApplicataCell Line TumorE-selectinmedicineCell AdhesionHumansCell adhesionSialyl Lewis X AntigenTube formationOvarian NeoplasmsNeovascularization PathologicIntegrin beta1AutophagyEndothelial CellsCell BiologyOriginal Articlesmedicine.diseaseasparaginaseL-asparaginaseCell biologymedicine.anatomical_structureovarian cancersialyl Lewis Xbiology.proteinMolecular MedicineFemaleOvarian cancerE-Selectin
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IkappaB kinase 2 determines oligodendrocyte loss by non-cell-autonomous activation of NF-kappaB in the central nervous system

2011

The IκB kinase complex induces nuclear factor kappa B activation and has recently been recognized as a key player of autoimmunity in the central nervous system. Notably, IκB kinase/nuclear factor kappa B signalling regulates peripheral myelin formation by Schwann cells, however, its role in myelin formation in the central nervous system during health and disease is largely unknown. Surprisingly, we found that brain-specific IκB kinase 2 expression is dispensable for proper myelin assembly and repair in the central nervous system, but instead plays a fundamental role for the loss of myelin in the cuprizone model. During toxic demyelination, inhibition of nuclear factor kappa B activation by …

Central Nervous SystemBlotting WesternIκB kinaseBiologyddc:616.07Myelin assemblyMicroglia/cytology/metabolismNerve Regeneration/physiologyDemyelinating Diseases/chemically induced/metabolism03 medical and health sciencesMyelinCuprizoneMice0302 clinical medicineCentral Nervous System/cytology/metabolismmedicineAnimalsRemyelinationCHUKMyelin Sheath030304 developmental biologyAstrocytes/cytology/metabolismMyelin Sheath/metabolism0303 health sciencesReverse Transcriptase Polymerase Chain ReactionSignal Transduction/physiologyI-Kappa-B KinaseNF-kappa BI-kappa B Kinase/metabolismOriginal ArticlesOligodendrocyte3. Good healthCell biologyI-kappa B KinaseNerve RegenerationOligodendrogliamedicine.anatomical_structureOligodendroglia/metabolismAstrocytesNF-kappa B/metabolismNeurogliaNeurology (clinical)MicrogliaNeuroscience030217 neurology & neurosurgeryDemyelinating DiseasesSignal Transduction
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Physical, Psychological and Sexual Abuse of the Minor in the Families from the Northwestern Region of Romania- Social and Medical Forensics

2020

Background: In Romania, the abuse within the family of the minor child is a widespread phenomenon, its extent is insufficiently known because of ignorance/not reporting all the existing cases.
 Methods: The participants of the research are represented by two independent groups from the NW Romania 2007-2011, one for sociological study (1544 parents and 1283 children) and another for forensic statistical study (2761 cases of abused children). The sociological study was carried out by analyzing questionnaires applied in schools located in Bihor County, both to children and parents. The statistical analysis was carried out by studying the cases of the physically, sexually, and psychologica…

Child abuseRomaniamedia_common.quotation_subjectPublic Health Environmental and Occupational HealthIgnoranceMinor (academic)Abused childNeglectPhysical abuseSexual abuseRisk factorsOriginal ArticleFamilyRural areaPublic aspects of medicineRA1-1270PsychologyPsychological abusemedia_commonClinical psychologyIranian Journal of Public Health
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Association between malocclusion and articulation of phonemes in early childhood

2021

ABSTRACT Objectives To evaluate the relationship between dental malocclusion and speech to understand the etiology of speech sound disorders (SSD) in schoolchildren and to make a correct diagnosis and treatment plan. Materials and Methods Articulation and dental occlusion, oromyofunctional evaluation with orofacial praxis and musculature, resting tongue position, and swallowing pattern were analyzed in 290 schoolchildren between the ages of 4 and 7 years. Statistical tests were considered significant for P < .05. Results A significant association between dental malocclusions (Angle Class II and III, anterior open bite, edge-to-edge bite, overjet and anterior crossbite) and phonetic a…

Child PreschoolOpen BitePrevalenceHumansTongue HabitsOrthodonticsOriginal ArticlesMalocclusion Angle Class IIChildMalocclusionOverbiteThe Angle Orthodontist
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How consistent are the transcriptome changes associated with cold acclimation in two species of the Drosophila virilis group?

2015

This work was financially support by a Marie Curie Initial Training Network grant, “Understanding the evolutionary origin of biological diversity” (ITN-2008–213780 SPECIATION), grants from the Academy of Finland to A.H. (project 132619) and M.K. (projects 268214 and 272927), a grant from NERC, UK to M.G.R. (grant NE/J020818/1), and NERC, UK PhD studentship to D.J.P. (NE/I528634/1). For many organisms the ability to cold acclimate with the onset of seasonal cold has major implications for their fitness. In insects, where this ability is widespread, the physiological changes associated with increased cold tolerance have been well studied. Despite this, little work has been done to trace chang…

Chill-comaAcclimatizationQH301 BiologyDrosophila virilisStress toleranceGenes Insectta3111AcclimatizationTranscriptomeMyoinositolQH301Species SpecificityCulex-pipiensMelanogasterGeneticsMelanogasterCold acclimationAnimalsThermotaxisCircadian rhythmDifferential expression analysisGeneGenetics (clinical)Northern house mosquitoGeneticsbiologySequence Analysis RNAcold acclimationta1184TemperatureChromosome MappingLarge gene listsbiology.organism_classificationBiological-membranesCold TemperatureDrosophila virilisMultigene Familyta1181Original ArticleDrosophilaFemaleGenetic FitnessTranscriptomeHeredity
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The Sea Urchin sns5 Insulator Protects Retroviral Vectors From Chromosomal Position Effects by Maintaining Active Chromatin Structure

2009

Silencing and position-effect (PE) variegation (PEV), which is due to integration of viral vectors in heterochromatin regions, are considered significant obstacles to obtaining a consistent level of transgene expression in gene therapy. The inclusion of chromatin insulators into vectors has been proposed to counteract this position-dependent variegation of transgene expression. Here, we show that the sea urchin chromatin insulator, sns5, protects a recombinant gamma-retroviral vector from the negative influence of chromatin in erythroid milieu. This element increases the probability of vector expression at different chromosomal integration sites, which reduces both silencing and PEV. By chr…

Chromatin ImmunoprecipitationEuchromatinHeterochromatinGenetic VectorsSettore BIO/11 - Biologia MolecolareSettore MED/08 - Anatomia PatologicaBiologyChromatin remodelingChromosomal Position EffectsMiceCell Line TumorDrug DiscoveryGeneticsAnimalsNucleosomeGATA1 Transcription FactorPosition EffectChromatin insulatorMolecular BiologyChIA-PETGeneticsPharmacologyChromatin insulator; Position Effects; Histone modificationsHistone modificationsChromosomal Position EffectsOriginal ArticlesChromatinChromatinRetroviridaeSea UrchinsNIH 3T3 CellsMolecular MedicineInsulator ElementsChromatin immunoprecipitationOctamer Transcription Factor-1Protein BindingMolecular Therapy
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An Investigation of the Seasonal Pattern of Mannitol Content in Deciduous and Evergreen Species of the Oleaceae Growing in Northern Sicily

2002

In several species of the Oleaceae, mannitol, already present at considerable levels, accumulates in response to stress. This family comprises both deciduous and evergreen species, and we investigated the role of mannitol in deciduous malacophyll and evergreen sclerophyll species growing under the same conditions in the field. The relationship between mannitol content and changes in rainfall or temperature was also studied. The mannitol content of leaves of Fraxinus ornus L., F. angustifolia Vahl., Olea europaea L. and Phillyrea media L. was determined by gas chromatography. Leaf samples were collected once a month for 1 year. In the two ash species, the seasonal pattern of mannitol content…

Chromatography GasAcclimatizationClimateOleaceaeRainPlant ScienceFraxinus angustifoliaFraxinusDisastersBotanyFraxinus ornus Fraxinus angustifolia Olea europaea Phillyrea media ash drought gas chromatography mannitol Oleaceae olive seasonal variation stressmedicineSettore BIO/04 - Fisiologia VegetaleMannitolSicilybiologyTemperatureOriginal ArticlesEvergreenPhillyreabiology.organism_classificationPlant LeavesDeciduousOleaOleaceaeMannitolSeasonsmedicine.drug
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Changes in Speech Range Profile Are Associated with Cognitive Impairment

2021

Background and Purpose The aim of this study was to describe the variations in the speech range profile (SRP) of patients affected by cognitive decline. Methods We collected the data of patients managed for suspected voice and speech disorders, and suspected cognitive impairment. Patients underwent an Ear Nose and Throat evaluation and Mini-Mental State Examination (MMSE). To obtain SRP, we asked the patients to read 18 sentences twice, at their most comfortable pitch and loudness as they would do in daily conversation, and recorded their voice on to computer software. Results The study included 61 patients. The relationship between the MMSE score and SRP parameters was established. Increas…

Cognitive Impairmentmedicine.medical_specialtyRange (music)business.industryEar nose and throatCognitionAudiologymedicine.diseaseMental Status and Dementia Testsenvironment and public healthLoudnessComputer softwareotorhinolaryngologic diseasesMedicineDementiaSpeechOriginal ArticleDementiaCognitive declinebusinessCognitive impairmentLanguageDementia and Neurocognitive Disorders
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Listening understanding and acting (lung): focus on communicational issue in thoracic oncology.

2019

Background: In the field of oncological assistance, nowadays we have to deal with a complex scenario where patients got used to obtain a huge amount of information through internet or social media and to apply them in performing their health-related decisions. This landscape requires that clinicians become able to handle therapeutical approaches and adequate skills in communication tools to satisfy the current needs. Our project aimed to build a communication model based on clinical oncologists’ real experiences in order to find a simple way to share with patients all the innovative therapeutical opportunities today available in lung cancer. The final goal is to design a flexible and person…

Communication Internet Lung cancerCancer ResearchKnowledge managementComputer sciencemedia_common.quotation_subjectContext (language use)Quality of life (healthcare)Nuclear Medicine and ImagingRadiology Nuclear Medicine and imagingActive listeningSocial mediaPeer learningmedia_commonTeamworkbusiness.industryCommunicationCommunication; Internet; Lung cancer; Oncology; Radiology Nuclear Medicine and Imaging; Cancer Researchlung cancerCommunication; lung cancer; internetOncologyModels of communicationThe InternetOriginal ArticleinternetbusinessRadiologyTranslational cancer research
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The use of complementary alternative medicines in advanced cancer patients followed at home

2021

Objectives: To assess the use of complementary alternative medicines (CAM) in advanced cancer patients followed at home. Methods: A consecutive sample of advanced cancer patients admitted to a home palliative care program was invited to participate in the study in a period of two months. Demographic characteristics, religious belief, education level, diagnosis, and socio-economic condition were recorded. Patients were asked about the use of CAM, considered to be a health-related treatment practiced outside the established health services. Information about CAM indications and those who prescribed or suggested CAM were also collected. Results: Two hundred and eighty-three advanced cancer pat…

Complementary Therapiesmedicine.medical_specialtyPalliative carePain medicineHome careCONSECUTIVE SAMPLEHealth servicesComplementary alternative medicineSurveys and QuestionnairesAdvanced cancerNeoplasmsmedicineHumansSurveys and Questionnairebusiness.industryNursing researchReligious beliefAdvanced cancerHome Care ServicesOncologyComplementary TherapieFamily medicinePalliative careOriginal ArticlebusinessHuman
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