Search results for "P2"
showing 10 items of 871 documents
Influence of CYP2C9 polymorphism on serum levels of phenobarbital metabolites
2013
ATTIVITÀ ANTIPROLIFERATIVA DI UN DERIVATO POLICICLICO CON STRUTTURA COMPLESSA
2014
Un nuovo derivato policiclico glicosilato è stato ottenuto partendo dal derivato policiclico iodurato, utilizzando la reazione di Sonogashira.Il composto glicosilato saggiato presso l’NCI è risultato attivo su tutte le 60 linee cellulari tumorali del panel, risultando più attivo degli altri composti policiclici precedentemente saggiati. Il composto induce l’arresto del ciclo cellulare in fase G2/M nella linea cellulare MDA-MB231, fa diminuire i livelli di ciclina B1e Cdc-2 mentre produce un aumento dell’inibitore p21WAF1, una chinasi ciclino-dipendente.
Regulatory networks underlying mycorrhizal development delineated by genome-wide expression profiling and functional analysis of the transcription fa…
2017
Background: Ectomycorrhizal (ECM) fungi develop a mutualistic symbiotic interaction with the roots of their host plants. During this process, they undergo a series of developmental transitions from the running hyphae in the rhizosphere to the coenocytic hyphae forming finger-like structures within the root apoplastic space. These transitions, which involve profound, symbiosis-associated metabolic changes, also entail a substantial transcriptome reprogramming with coordinated waves of differentially expressed genes. To date, little is known about the key transcriptional regulators driving these changes, and the aim of the present study was to delineate and functionally characterize the trans…
Transport of Arabidopsis GPI-anchored proteins along the early secretory pathway: Role of GPI-anchor remodelling and p24 proteins
2019
Las proteínas con anclaje GPI son una familia de proteínas que están ancladas a la cara exterior de la membrana plasmática mediante un ancla GPI y se ha visto que juegan un papel clave en plantas. Sin embargo, la maquinaria molecular implicada en el transporte de estas proteínas a la membrana plasmática en plantas es aún desconocida. Las proteínas p24 tienen un papel importante en el control de calidad de proteínas (posiblemente incluyendo las proteínas con anclaje GPI) transportadas entre el Retículo Endoplasmático y el aparato de Golgi. Esta tesis doctoral tiene como objetivo el estudio de la posible implicación de las proteínas p24 en la salida del Retículo Endoplasmático y en la localiz…
Activation of NF-kappaB and IL-8 by yersinia enterocolitica invasin protein is conferred by engagement of rac1 and MAP kinase cascades.
2003
International audience; Yersinia enterocolitica triggers activation of the nuclear factor (NF)-kappaB and production of the proinflammatory chemokine interleukin (IL)-8 in intestinal epithelial cells. This activation is due to adhesion of the bacteria via their outer membrane protein invasin to the host cells. Using Clostridium difficile toxins that specifically inactivate small GTPases, and transfection of inhibitory proteins of the Rho-GTPases, we demonstrate that Rac1, but not Cdc42 or Rho, is required for activation of NF-kappaB by invasin. Invasin activated the mitogen activated protein kinases (MAPK) p38 and c-Jun N-terminal protein kinase (JNK) but not extracellular signal regulated …
Rac1 and PAK1 are upstream of IKK-ε and TBK-1 in the viral activation of interferon regulatory factor-3
2004
The anti-viral type I interferon (IFN) response is initiated by the immediate induction of IFN beta, which is mainly controlled by the IFN-regulatory factor-3 (IRF-3). The signaling pathways mediating viral IRF-3 activation are only poorly defined. We show that the Rho GTPase Rac1 is activated upon virus infection and controls IRF-3 phosphorylation and activity. Inhibition of Rac1 leads to reduced IFN beta promoter activity and to enhanced virus production. As a downstream mediator of Rac signaling towards IRF-3, we have identified the kinase p21-activated kinase (PAK1). Furthermore, both Rac1 and PAK1 regulate the recently described IRF-3 activators, I kappa B kinase- and TANK-binding kina…
Rakenteen ja kokemuksen komplementaarisuus laadullisen aineiston tulkinnassa ja analyysissa
2019
Artikkelissa käsitellään yhteiskuntatieteiden tutkimusmetodologiassa esiintyvää, yhteiskunnallishistoriallisten rakenteiden ja yksilön kokemusten välistä rakennetta. Se kiinnittyy hallinnan ja ideologioiden kriittisen tutkimuksen metodologiaan. Ongelmakentän tarkentamiseksi analysoidaan kahden tutkimusperinteen tarjoamia välineitä rakenteen ja kokemuksen yhdistämiseen tutkimusmetodiikassa. Perinteet ovat hallinnan analytiikka ja psykoanalyyttinen, yksilöllisiä kokemuksia painottava tutkimustraditio. Erityisesti tarkastellaan mahdollisuutta tuottaa analyysia, jossa otetaan huomioon sekä yhteiskunnan rakenteet että yksilön kokemus. Empiirisenä esimerkkinä käytetään vuonna 2015 valmistunutta t…
Papel de la fosforilación de p27 en la serina 10 en la función endotelial y el remodelado vascular patológico
2015
El supresor tumoral p27 ha sido ampliamente implicado en multitud de procesos patológicos donde ejerce un papel protector debido a su acción antiproliferativa. Particularmente, a nivel cardiovascular, nuestro laboratorio ha demostrado que p27 protege frente al desarrollo de aterosclerosis en modelos murinos gracias a su función anti-proliferativa (1, 2). La regulación de p27 está fuertemente mediada por sus modificaciones postraduccionales, entre las que destaca su fosforilación en la serina 10 (principal modificación que sufre la proteína). Recientemente, nuestro laboratorio ha demostrado que la fosforilación de p27 en su Ser10 protege frente al desarrollo de aterosclerosis avanzada, incre…
Chronic ethanol exposure alters the levels, assembly, and cellular organization of the actin cytoskeleton and microtubules in hippocampal neurons in …
2010
The organization and dynamics of microtubules (MTs) and the actin cytoskeleton are critical for the correct development and functions of neurons, including intracellular traffic and signaling. In vitro ethanol exposure impairs endocytosis, exocytosis, and nucleocytoplasmic traffic in astrocytes and alters endocytosis in cultured neurons. In astrocytes, these effects relate to changes in the organization and/or function of MTs and the actin cytoskeleton. To evaluate this possibility in hippocampal cultured neurons, we analyzed if chronic ethanol exposure affects the levels, assembly, and cellular organization of both cytoskeleton elements and the possible underlying mechanisms of these effec…
AGE6. Skin Ageing: Focus on the Role of Inflammatory Genetic Factors in Cutaneous Neoplasia
2014
AGE6. Skin Ageing: Focus on the Role of Inflammatory Genetic Factors in Cutaneous Neoplasia C. M. Gambino1, F. Crapanzano1, G. Accardi1, A. Aiello1, C. Virruso1, G. Pistone1, M. R. Bongiorno1, D. Lio1, C. R. Balistreri1, G. Candore1 1University of Palermo, Palermo, Italy Background: Skin aging is a complex process that involves intrinsic and exogenous causes. Photo-oxidative damage caused by UV is the leading cause of extrinsic aging of the skin, known as photo-ageing. UV damages can be linked mostly to overproduction of ROS that induces a complex molecular cascade able to accelerate physiological aging, determining a typical dermal/epidermal inflammation with an increased risk of getting s…