Search results for "PBS"
showing 10 items of 31 documents
Genetic Structure of the Limecola Balthica Population in the Gulf of Riga, Baltic Sea
2020
Abstract Samples of Limecola balthica with normal and deformed shells were collected from ten sites throughout the Gulf of Riga. Genetic diversity was evaluated by the retrotransposon-based iPBS method. Samples had close mutual genetic distances, which showed that all of them belong to one wider population of the Gulf of Rīga. No direct relationship between the activity of retrotransposons and deformation of shells was found.
ICTV Virus Taxonomy Profile: Pseudoviridae
2021
Pseudoviridae is a family of reverse-transcribing viruses with long terminal repeats (LTRs) belonging to the order Ortervirales. Pseudoviruses are commonly found integrated in the genomes of diverse plants, fungi and animals and are broadly known as Ty1/Copia LTR retrotransposons. Inside the cell, they form icosahedral virus particles, but unlike most other viruses, do not have an extracellular phase. This is a summary of the ICTV Report on the family Pseudoviridae, which is available at ictv.global/report/pseudoviridae.
Polyoxypregnanes as safe, potent, and specific ABCB1-inhibitory pro-drugs to overcome multidrug resistance in cancer chemotherapy in vitro and in vivo
2021
Multidrug resistance (MDR) mediated by ATP binding cassette subfamily B member 1 (ABCB1) is significantly hindering effective cancer chemotherapy. However, currently, no ABCB1-inhibitory drugs have been approved to treat MDR cancer clinically, mainly due to the inhibitor specificity, toxicity, and drug interactions. Here, we reported that three polyoxypregnanes (POPs) as the most abundant constituents of Marsdenia tenacissima (M. tenacissima) were novel ABCB1-modulatory pro-drugs, which underwent intestinal microbiota-mediated biotransformation in vivo to generate active metabolites. The metabolites at non-toxic concentrations restored chemosensitivity in ABCB1-overexpressing cancer cells v…
Towards metal chalcogenide nanowire-based colour-sensitive photodetectors
2018
Financial support provided by Scientific Research Project for Students and Young Researchers Nr. SJZ/2016/6 realized at the Institute of Solid State Physics, University of Latvia is greatly acknowledged. Authors are grateful to Reinis Ignatans for XRD measurements.
Microfibrillar polymeric ocular inserts for triamcinolone acetonide delivery.
2019
Abstract Despite eye drops generally represent the most convenient, simple and patient-friendly formulations to treat ocular diseases, they suffer from poor retention on the ocular surface and low drug bioavailability leading to the necessity of prolonged and continuous treatment over time. Therefore, ocular insert could represent an innovative way to benefit from ocular topical administration while minimizing all the relevant limitation related to this route of administration. Polymeric non-erodible mucoadhesive ocular inserts should be comfortable and should rapidly adhere on the ocular surface, remain in situ for prolonged period, assure a reproducible and controlled drug release as well…
RAB3GAP1 and RAB3GAP2 modulate basal and rapamycin-induced autophagy
2014
Macroautophagy is a degradative pathway that sequesters and transports cytosolic cargo in autophagosomes to lysosomes, and its deterioration affects intracellular proteostasis. Membrane dynamics accompanying autophagy are mostly elusive and depend on trafficking processes. RAB GTPase activating proteins (RABGAPs) are important factors for the coordination of cellular vesicle transport systems, and several TBC (TRE2-BUB2-CDC16) domain-containing RABGAPs are associated with autophagy. Employing C. elegans and human primary fibroblasts, we show that RAB3GAP1 and RAB3GAP2, which are components of the TBC domain-free RAB3GAP complex, influence protein aggregation and affect autophagy at basal an…
Genetic diversity of two perch Perca fluviatilis populations of the Latgale region
2015
Molecular markers based on retrotransposons possibility of integration into genomes of many organisms are commonly used for genetic analysis of different species. The aim of this study was to test possibility of use those markers in perch and detect genetic diversity of populations of two lakes of the Latgale region of Latvia: Cirišs and Sventes. The distance between the lakes is nearly 60 km, they belong to the same Daugava river basin but are different from ecological point of view. Forty two blood samples of <em>Perca fluviatilis </em>were collected altogether. Extracted DNA was analyzed using inter-PBS amplification technique with specifically selected retrotransposon-based …
Baltijas plakangliemenes Macoma balthica populācijas ģenētiskās daudzveidības analīze Rīgas jūras līča piekrastē
2015
Bakalaura darbā pētīta baltijas plakangliemeņu (Macoma balthica) populācijas ģenētiskā daudzveidība Rīgas jūras līča piekrastes zonā. Izmantojot iPBS (inter - Primer Binding Sequences) metodi veikta 12 dažādu atradņu gliemeņu ģenētiskās daudzveidības analīze. PCR amplifikācijas produkti, pēc reakcijas ar diviem praimeriem (2232 un 2394), tika analizēti agarozes gēlā. Kopā tika konstatēti 38lokusi. Darbā analizētajiem paraugiem konstatēts augsts polimorfisma līmenis. Salīdzinot atradnes savā starpā, polimorfu lokusu skaits - 22 (59 %). Ģenētiskā līdzība visām populācijām ir augstāka par 88 %. Iegūtie dati tika matemātiski apstrādāti ar POPGENE un NTSYS programmnodrošinājumu.
The LepR-mediated leptin transport across brain barriers controls food reward
2018
Objective Leptin is a key hormone in the control of appetite and body weight. Predominantly produced by white adipose tissue, it acts on the brain to inhibit homeostatic feeding and food reward. Leptin has free access to circumventricular organs, such as the median eminence, but entry into other brain centers is restricted by the blood–brain and blood–CSF barriers. So far, it is unknown for which of its central effects leptin has to penetrate brain barriers. In addition, the mechanisms mediating the transport across barriers are unclear although high expression in brain barriers suggests an important role of the leptin receptor (LepR). Methods We selectively deleted LepR in brain endothelia…
Charge Transport in Trap-Sensitized Infrared PbS Quantum-Dot-Based Photoconductors: Pros and Cons
2018
Control of quantum-dot (QD) surface chemistry offers a direct approach for the tuning of charge-carrier dynamics in photoconductors based on strongly coupled QD solids. We investigate the effects of altering the surface chemistry of PbS QDs in such QD solids via ligand exchange using 3-mercaptopropionic acid (MPA) and tetrabutylammonium iodide (TBAI). The roll-to-roll compatible doctor-blade technique was used for the fabrication of the QD solid films as the photoactive component in photoconductors and field-effect phototransistors. The ligand exchange of the QD solid film with MPA yields superior device performance with higher photosensitivity and detectivity, which is due to less dark cur…