Search results for "PC"

showing 10 items of 2805 documents

Citrus rootstock breeding: response of four allotetraploid somatic hybrids to Citrus tristeza virus induced infections

2018

Four allotetraploid somatic hybrids of citrus, with potential for rootstock improvement, have been evaluated for their response to Citrus tristeza virus (CTV) infection. CTV is the most important viral pathogen affecting citrus production worldwide. Somatic combinations of ‘Milam’ lemon (Citrus jambhiri Lush.) + Sour orange (C. aurantium L Osb.), Calamondin (C. madurensis Lour.) + ‘Keen’ sour orange (C. aurantium L.), Calamondin + ‘Femminello‘ lemon (C. limon L. Burm. F.) and Cleopatra mandarin (C. reshni Hort. ex Tan.) + ‘Femminello’ lemon, were studied. Plants were grafted with CTV-infected “Valencia” sweet orange budwood. Two different CTV strains collected in Sicily, considered as “mild…

0106 biological sciences0301 basic medicineReal-time qRT-PCRRT-PCRProtoplast fusionPlant ScienceOrange (colour)Horticulture01 natural sciencesMediterranean Basin03 medical and health sciencesstomatognathic systemGenotypeCitrus rootstockHybridRootstocksbiologyRough lemonSettore AGR/12 - Patologia VegetaleCitrus tristeza virusfood and beveragesbiology.organism_classificationCTV. Protoplast fusion . Rootstocks . DASELISA. RT-PCR . Real-time qRT-PCRHorticulture030104 developmental biologyCTVRootstockDAS-ELISAAgronomy and Crop Science010606 plant biology & botany
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Exploring species-level taxonomy in the Cryptocephalus flavipes species complex (Coleoptera: Chrysomelidae)

2016

In insects, morphological species identification is often challenging. The discrimination of closely related species may be hampered when only subtle differences in phenotypic characters or a continuum in their variability are present. This is exemplified in the Cryptocephalus flavipes species complex (Coleoptera; Chrysomelidae) where, until now, the species have been discriminated only by the yellow pattern on frons, pronotum, and epipleurae. In the present study, the phylogeny of the C. flavipes species complex was resolved through a multi-locus sequence approach, and the inclusion in the group of the phenotypically similar Cryptocephalus quadripustulatus Gyllenhal, 1813 was evaluated. Su…

0106 biological sciences0301 basic medicineSpecies complexgeometric morphometricZoologyCryptocephalus quadripustulatus010603 evolutionary biology01 natural sciencesnew ISS rRNA PCR primers03 medical and health sciencesNew 18SrRNA PCR primerSpecies levelPhylogeneticsSpecies delimitationLeaf beetleDNA taxonomyEcology Evolution Behavior and SystematicsTaxonomybiologyBiodiversitybiology.organism_classificationCryptocephalus flavipes030104 developmental biologyTaxonspecies deliminationAnimal Science and ZoologyTaxonomy (biology)Leaf beetleZoological Journal of the Linnean Society
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Thioredoxin (Trxo1) interacts with proliferating cell nuclear antigen (PCNA) and its overexpression affects the growth of tobacco cell culture.

2017

Thioredoxins (Trxs), key components of cellular redox regulation, act by controlling the redox status of many target proteins, and have been shown to play an essential role in cell survival and growth. The presence of a Trx system in the nucleus has received little attention in plants, and the nuclear targets of plant Trxs have not been conclusively identified. Thus, very little is known about the function of Trxs in this cellular compartment. Previously, we studied the intracellular localization of PsTrxo1 and confirmed its presence in mitochondria and, interestingly, in the nucleus under standard growth conditions. In investigating the nuclear function of PsTrxo1 we identified proliferati…

0106 biological sciences0301 basic medicineTFs transcription factorsOverexpressionBiologíaBiFC bimolecular fluorescence complementationClinical BiochemistryCell Culture TechniquesTobacco BY-2 cells01 natural sciencesBiochemistryTBY-2 tobacco bright yellow-2DTT 14-dithiothreitolBimolecular fluorescence complementationThioredoxinsGene Expression Regulation PlantTrx thioredoxinlcsh:QH301-705.5GFP green fluorescent proteinlcsh:R5-920biologyProliferating cell nuclear antigen (PCNA)Cell cycleGlutathione3. Good healthCell biologyMitochondriaNTR NADPH thioredoxin reductaseProtein TransportDEM diethyl maleateRT-qPCR Reverse transcription quantitative polymerase chain reactionThioredoxinlcsh:Medicine (General)Oxidation-ReductionAMS 4-acetamido-4-maleimidylstilbene-22-disulfonic acidResearch PaperPCNA proliferating cell nuclear antigenOex overexpressingCell cycleNucleusThioredoxin o103 medical and health sciencesROS reactive oxygen speciesDownregulation and upregulationProliferating Cell Nuclear AntigenTobaccoDAPI 46-diamidine-2-phenylindolmCBM monochlorobimaneCellular compartmentCell NucleusCell growthOrganic ChemistryBotánicaPeasMolecular biologyYFP yellow fluorescent proteinProliferating cell nuclear antigenTBS Tris-buffered salineOD optical density030104 developmental biologylcsh:Biology (General)Cell cultureRNA reactive nitrogen speciesbiology.proteinPrx peroxiredoxinBSA bovine serum albumin010606 plant biology & botanyRedox biology
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A comparative study of viral infectivity, accumulation and symptoms induced by broad bean wilt virus 1 isolates

2018

Broad bean wilt virus 1 (BBWV-1, genus Fabavirus, family Secoviridae) is a bipartite positive-sense single-stranded RNA virus distributed worldwide infecting many herbaceous species. Until now, scarce information regarding biological properties of BBWV-1 isolates is available. This work shows a comparative study on virus infectivity (proportion of infected plants over inoculated plants), virus accumulation and symptoms induced by four genetically different BBWV-1 isolates (Ben, B41/99, NSRV and PV0548) which were mechanically inoculated on several herbaceous hosts. The four BBWV-1 isolates infected broad bean, tomato, pepper and Nicotiana benthamiana plants, whereas none of them infected cu…

0106 biological sciences0301 basic medicinevirusesNicotiana benthamianaPlant Science01 natural sciencesBBWV-1VirusMicrobiologyFabaviru03 medical and health sciencesBroad bean wilt virusPlant virusPepperSecoviridaeSymptomatologyInfectivitybiologySecoviridaeHost (biology)RT-qPCRfungiSettore AGR/12 - Patologia Vegetalefood and beveragesbiology.organism_classification030104 developmental biologyInfectivity010606 plant biology & botanyJournal of Plant Pathology
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Phylogenomics Identifies an Ancestral Burst of Gene Duplications Predating the Diversification of Aphidomorpha

2019

Aphids (Aphidoidea) are a diverse group of hemipteran insects that feed on plant phloem sap. A common finding in studies of aphid genomes is the presence of a large number of duplicated genes. However, when these duplications occurred remains unclear, partly due to the high relatedness of sequenced species. To better understand the origin of aphid duplications we sequenced and assembled the genome of Cinara cedri, an early branching lineage (Lachninae) of the Aphididae family. We performed a phylogenomic comparison of this genome with 20 other sequenced genomes, including the available genomes of five other aphids, along with the transcriptomes of two species belonging to Adelgidae (a close…

0106 biological sciences:Informàtica::Aplicacions de la informàtica::Bioinformàtica [Àrees temàtiques de la UPC]Gene duplicationAphidomorphaLineage (evolution)010603 evolutionary biology01 natural sciencesGenomeSyntenyDNA sequencingFilogèniaEvolution Molecular03 medical and health sciencessequencia genómicaSpecies SpecificityPhylogenomicsGene duplicationBioinformaticaGeneticsAdelgidaeAnimalsMolecular BiologyEcology Evolution Behavior and SystematicsDiscoveriesPhylogeny030304 developmental biologySegmental duplication0303 health sciencesAphidbiologyWhole Genome SequencingGene Expression Profilinggene duplicationfood and beveragesHigh-Throughput Nucleotide SequencingAfidomorfabiochemical phenomena metabolism and nutritionbiology.organism_classificationaphidsGenòmicaGene Expression RegulationEvolutionary biologyAphidsInsect ProteinsGenèticaMolecular Biology and Evolution
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DNA-Based Identification of Larvae Offers Insights into the Elusive Lifestyles of Native Olive Seed Wasps in South Africa

2020

Wild and cultivated olives in the Western Cape of South Africa are direct or indirect hosts to a high diversity of Braconidae and Chalcidoidea wasps. Olive-associated Braconidae are known to parasitise the larvae of Bactrocera oleae (Rossi), and probably also Bactrocera biguttula (Bezzi). The lifestyle of olive-associated Chalcidoidea is not fully understood, and may include phytophagous, parasitoid and hyperparasitoid species. Some chalcids could represent olive seed wasps (OSW), a generic term that designates the seed feeders responsible for losses in commercial olive production. In order to obtain direct DNA-based evidence for the lifestyle of four putative OSW – Eupelmus spermophilus Si…

0106 biological sciencesBactrocera biguttulaLarvabiologySpermophilus010607 zoologyZoologybiology.organism_classification01 natural sciencesChalcidoidea Eupelmus spermophilus Eurytoma oleae Eurytoma varicolor Neochrysocharis formosus Olea europaea PCR multiplex Sycophila aethiopicaParasitoid010602 entomologyNeochrysocharisSettore AGR/11 - Entomologia Generale E ApplicataInsect ScienceBactroceraIdentification (biology)Agronomy and Crop ScienceBraconidaeEcology Evolution Behavior and SystematicsAfrican Entomology
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Differential Effect of Plant Lipids on Membrane Organization

2015

SPE IPM; International audience; The high diversity of the plant lipid mixture raises the question of their respective involvement in the definition of membrane organization. This is particularly the case for plant plasma membrane, which is enriched in specific lipids, such as free and conjugated forms of phytosterols and typical phytosphingolipids, such as glycosylinositolphosphoceramides. This question was here addressed extensively by characterizing the order level of membrane from vesicles prepared using various plant lipid mixtures and labeled with an environment-sensitive probe. Fluorescence spectroscopy experiments showed that among major phytosterols, campesterol exhibits a stronger…

0106 biological sciencesCampesterolMembrane lipidsBiologyMembrane Reconstitution01 natural sciencesBiochemistry03 medical and health scienceschemistry.chemical_compoundMicroscopic ImagingMembrane fluidity[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process EngineeringMolecular BiologyLipid raft030304 developmental biologySterol0303 health sciencesVesicleCell BiologySphingolipidPhospholipid VesicleGIPCSterolMembranechemistryBiochemistryConjugated Forms of PhytosterolGlycerosphingolipidlipids (amino acids peptides and proteins)Lipid Rafts010606 plant biology & botany
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Fig virus-free production and survival rate improvement using meristem tip culture techinique associated with the encapsulation technology

2018

Three Mediterranean F. carica genotypes, i.e. cultivars Palazzo, Bifera nera and Catalanisca, initially infected by Fig leaf mottle-associated virus 1 (FLMaV-1), Fig leaf mottle-associated virus 2 (FLMaV-2), Fig mild mottling-associated virus (FMMaV), Fig mosaic virus (FMV), Fig latent virus 1 (FLV-1), Fig Badnavirus 1 (FBV-1) and Fig fleck-associated virus (FFkaV), were subjected to the sanitation technique via Meristem Tip (0.3-0.5 mm in size) Culture (MTC), also associated with the encapsulation technique (MTC-SS), in order to produce virus-free plant material. Encapsulation was tested to overcome the very low survival and regeneration rates, due to the small propagule size. Encouraging …

0106 biological sciencesChemistryfig mosaic disease synthetic seed sanitation RT-PCR.Settore AGR/12 - Patologia Vegetale04 agricultural and veterinary sciencesHorticultureMeristem01 natural sciencesEncapsulation (networking)Cell biologySettore AGR/03 - Arboricoltura Generale E Coltivazioni Arboree040103 agronomy & agriculture0401 agriculture forestry and fisheriesVirus freeSurvival rate010606 plant biology & botany
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Biotrickling filter modeling for styrene abatement. Part 1: Model development, calibration and validation on an industrial scale

2017

Abstract A three-phase dynamic mathematical model based on mass balances describing the main processes in biotrickling filtration: convection, mass transfer, diffusion, and biodegradation was calibrated and validated for the simulation of an industrial styrene-degrading biotrickling filter. The model considered the key features of the industrial operation of biotrickling filters: variable conditions of loading and intermittent irrigation. These features were included in the model switching from the mathematical description of periods with and without irrigation. Model equations were based on the mass balances describing the main processes in biotrickling filtration: convection, mass transfe…

0106 biological sciencesConvectionEngineeringEnvironmental EngineeringHealth Toxicology and MutagenesiseducationBiotrickling filter010501 environmental sciencesResidence time (fluid dynamics)01 natural scienceslaw.invention:Enginyeria química::Química del medi ambient::Química atmosfèrica [Àrees temàtiques de la UPC]Filter (large eddy simulation)Bioreactorslaw010608 biotechnologyMass transferEnvironmental ChemistryGases - PurificationVolatile organic compoundDiffusion (business)FiltrationStyrene0105 earth and related environmental scienceschemistry.chemical_classificationgeographyAir PollutantsVolatile Organic Compoundsgeography.geographical_feature_categorybusiness.industryPublic Health Environmental and Occupational HealthEnvironmental engineeringGeneral MedicineGeneral ChemistryPilot unitModels TheoreticalVolatile organic compoundInletPollutionGasos - DepuracióBiodegradation EnvironmentalchemistryCalibrationMathematical modelingBiological air treatmentbusinessFiltration
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The cost of open access publication of papers by spanish authors in five areas of the social sciences

2021

[ES] La publicación en abierto de los artículos conlleva unos gastos conocidos como cargos por procesamiento de artículos (APC, en sus siglas en inglés). El objetivo de este trabajo es determinar el coste de los artículos publicados en abierto mediante APC de autoría española en cinco categorías temáticas de las ciencias sociales: Humanities (Multidisciplinary), Sociology, Information Science & Library Science, Education & Educational Research y Communication de la Colección Principal de la Web of Science (WoS) durante el periodo 2012-2019. Se han identificado las revistas, las instituciones financiadoras y los modelos de publicación más frecuentes. El estudio indica que de las 223 revistas…

0106 biological sciencesCultural StudiesSociology and Political ScienceTotal costArticle processing chargeLibrary scienceBIBLIOTECONOMIA Y DOCUMENTACIONlcsh:A01 natural sciencesInformation scienceSocial sciencesapcGeneral Works03 medical and health sciencesOpen AccessPublicación en abierto010608 biotechnologyPolitical scienceAmedia_common.cataloged_instanceCiencias socialesEuropean unionmedia_common0303 health sciencesbiology030306 microbiologybusiness.industryGeneral Arts and Humanitiesciencias socialesEurosScientometricsbiology.organism_classificationAPCEducational researchCargo por procesamiento de artículosPublishingArticle Processing Chargeslcsh:General Workspublicación en abiertobusinesscargo por procesamiento de artículosArbor: Ciencia, Pensamiento y Cultura
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