Search results for "PCR"

Genetic relationship between clinical and environmental Vibrio cholerae isolates in Tanzania: A comparison using repetitive extragenic palindromic (R…

2015

The bacterium causing cholera, Vibrio cholerae, is a marine organism and coastal waters are important reservoirs of the organism. There are more than 200 serogroups of V. cholerae, of which serogroups O1 and O139 are known to be the causative agent of the cholera. The main virulent factor in V. cholerae is cholera toxin gene (ctx) that is found from the epidemic O1 and O139 strains, but may also be found in some strains other than O1 and O139 (non-O1 and non-O139). In this study, 48 V. cholerae strains isolated from three estuaries of Tanzania and 20 stool isolates were characterized in terms of their serogroups and possession of ctx gene and then compared using two PCR based fingerprinting…

Expression and characterization of the human sweet taste receptor expressed in a mammalian inducible cell line

2018

International audience; Sweet taste perception is mediated by a heterodimeric receptor composed of the two distinct protein subunits, TAS1R2 and TAS1R3. TAS1R2 and TAS1R3 subunits are members of the small family of class C GPCRs. Class C GPCRs share a large N-terminal domain (NTD) linked to the heptahelical transmembrane domain by a cysteine-rich region. TAS1R2/TAS1R3 is the primary receptor for a diverse range of sweet compounds including natural sugars, sweet amino acids, artificial sweeteners and plant sweet-tasting proteins. In order to understand the molecular mechanisms that govern receptor – ligand interactions and the relative contribution of the two subunits to the detection of swe…

CHARACTERIZATION OF ATYPICAL CLAVIBACTER MICHIGANENSIS subsp. MICHIGANENSIS POPULATIONS IN GREENHOUSE TOMATOES IN ITALY

2012

The quarantine bacterium Clavibacter michiganensis subsp. michiganensis (Cmm) is the agent of tomato bacterial canker (TBC), one of the most destructive bacterial diseases of this crop, that causes severe economic losses worldwide. During a recent outbreak of TBC in Sicily (insular Italy), it was impossible to amplify the pat-1 gene by PCR with CMM5/CMM6 primers from affected greenhouse-grown plants from three farms, although Cmm-like colonies were consistently isolated. Microlog metabolic profile, 16S rDNA sequencing and the positive amplification of other Cmm DNA targets supported the identification of the strains as Cmm, suggesting the occurrence of a population lacking the virulence gen…

A single tube PCR assay for detecting viruses and their recombinants that cause tomato yellow leaf curl disease in Mediterranean basin

2007

Tomato yellow leaf curl disease (TYLCD) is well known in Mediterranean countries, where it has been causing severe losses in tomato crops for decades. Until recently, two viruses (with several isolates) in the genus Begomovirus, family Geminiviridae, have been associated with the epidemics: Tomato yellow leaf curl virus (TYLCV) and Tomato yellow leaf curl Sardinia virus (TYLCSV). However, recombinants between these, such as Tomato yellow leaf curl Malaga virus (TYLCMalV), are spreading, and new methods for detecting all viruses present in the region are needed. By considering all DNA sequences available of viruses causing TYLCD in the Mediterranean basin, a PCR/RFLP protocol was developed t…

Rivelazione e Caratterizzazione di Consorzi Microbici in Reperti Lignei Sommersi

2009

In questa lavoro sono state affrontate le problematiche relative alla valutazione dello stato di conservazione del legno archeologico sommerso (waterlogged wood) in relazione ai fattori che ne inducono il degrado. L’attenzione è stata rivolta ai processi di biodeterioramento, indotti dall’attività di alcuni microrganismi che utilizzano le componenti principali del legno, la cui identificazione è stata eseguita ricorrendo a tecniche sia colturali sia molecolari. In particolare, le tecniche molecolari che si sono rivelate di immediata applicazione per lo studio e la caratterizzazione dei microrganismi che colonizzano i beni di interesse storico artistico e i manufatti d’interesse archeologico…

Toxicity of retene and pyrene on fish at different light conditions

2011

Współdziałanie nauczycieli i rodziców w eliminowaniu trudności wychowawczych

2013

Trudności wychowawcze z uczniami klas młodszych stanowią jeden z najważniejszych problemów współczesnej edukacji Zagadnienie to jest bardzo istotne, gdyż coraz więcej dzieci sprawia rożnego rodzaju problemy, z którymi nie mogą poradzie sobie zarowno rodzice, jak i nauczyciele W artykule zostaną przedstawione wyniki badan na temat współpracy nauczycieli z rodzicami wraz z propozycjami eliminowania negatywnych zachowań u uczniów Ważne jest przy tym, aby nauczyciele wspólnie z rodzicami podejmowali pracę z dziećmi sprawiającymi trudności wychowawcze

RNS biomarķieru analīze prostatas vēža pacientu plazmas un urīna ekstracelulārajās vezikulās

2022

Ekstracelulārās vezikulas (EVs) ir heterogēni, ar membrānu norobežoti pūslīši, kas satur dažādas makromolekulas, tai skaitā RNS. EVs izdala visu veidu organisma šūnas un tās atrodas visos cilvēka bioloģiskajos šķidrumos. Ir pierādīta to loma prostatas vēža (PV) attīstībā, progresijā un metastazēšanās procesos. Šajā darbā iekļauta astoņu potenciālo RNS biomarķieru analīze PV un labdabīgas prostatas hiperplāzijas (LPH) pacientu plazmas un urīna EVs, kā arī PV pacientiem pirms un pēc radikālas prostatektomijas, izmantojot pilienu digitālo PCR. Tika novērota būtiska miR-375, piR-28004 un AMD1 līmeņa samazināšanās urīna EVs pēc radikālas prostatektomijas un tie tika izvirzīti kā potenciāli RNS b…