Search results for "PHAT"

showing 10 items of 4132 documents

Complete 1H, 13C{1H}, and 31P NMR Spectral Parameters of Some Pyrophosphates

2017

The 1H, 13C{1H}, and 31P NMR spectral parameters of some pyrophosphates were determined in CDCl3. The most complicated 1H spectrum can be solved fully only as (A3MN)R6XX′R6′(MNA3)′, where R6 (= ―N(CH3)2) is coupled only to phosphorus (X). Second-order coupling between phosphorus was found and solved with iterative analysis. A signal shape of one of the carbon resonance cannot be explained only with couplings. Explanation for exceptional shape was searched from molecular modeling results. peerReviewed

molecular modelingpyrophosphatesiterative spectral analysisNMRnuclear magnetic resonance spectroscopy
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Amorphous Polyphosphate and Ca‐Carbonate Nanoparticles Improve the Self‐Healing Properties of both Technical and Medical Cements

2020

Cement is used both as a construction material and for medical applications. Previously, it has been shown that the physiological polymer inorganic polyphosphate (polyP) is morphogenetically active in regeneration of skin, bone, and cartilage. The present study investigates the question if this polymer is also a suitable additive to improve the self-healing capacity not only of construction cement but also of inorganic bone void fillers. For the application in the cement, two different polyP-based amorphous nanoparticles (NP) are prepared, amorphous Ca-polyP NP and amorphous Ca-carbonate (ACC) NP. The particles are integrated into poly(methyl methacrylate) in a concentration ratio of 1:10. …

musculoskeletal diseases0106 biological sciencesMaterials sciencePolymersCarbonatesNanoparticle01 natural sciencesApplied Microbiology and BiotechnologyBone and Boneslaw.inventionchemistry.chemical_compoundlawPolyphosphates010608 biotechnologyCementchemistry.chemical_classificationConstruction MaterialsPolyphosphate010401 analytical chemistrytechnology industry and agricultureGeneral MedicinePolymerBone cementAmorphous calcium carbonate0104 chemical sciences3. Good healthAmorphous solidPortland cementsurgical procedures operativechemistryChemical engineeringMolecular MedicineNanoparticlesCalciumBiotechnology Journal
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Silicate modulates the cross-talk between osteoblasts (SaOS-2) and osteoclasts (RAW 264.7 cells): inhibition of osteoclast growth and differentiation

2012

It has been shown that inorganic monomeric and polymeric silica/silicate, in the presence of the biomineralization cocktail, increases the expression of osteoprotegerin (OPG) in osteogenic SaOS-2 sarcoma cells in vitro. In contrast, silicate does not affect the steady-state gene expression level of the osteoclastogenic ligand receptor activator of NF-κB ligand (RANKL). In turn it can be expected that the concentration ratio of the mediators OPG/RANKL increases in the presence of silicate. In addition, silicate enhances the growth potential of SaOS-2 cells in vitro, while it causes no effect on RAW 264.7 cells within a concentration range of 10-100 µM. Applying a co-cultivation assay system,…

musculoskeletal diseasesCell SurvivalCellular differentiationmedicine.medical_treatmentAcid PhosphataseMineralogyOsteoclastsCell Count02 engineering and technologyCell CommunicationBiochemistryCell Line03 medical and health sciencesMiceOsteoprotegerinOsteoclastOsteogenesismedicineAnimalsHumansMolecular BiologyRAW 264.7 Cells030304 developmental biologyTartrate-resistant acid phosphataseCell Proliferation0303 health sciencesOsteoblastsbiologyBone Density Conservation AgentsChemistryTartrate-Resistant Acid PhosphataseMacrophagesSilicatesRANK LigandCell DifferentiationCell Biology021001 nanoscience & nanotechnologyCoculture TechniquesCell biologyIsoenzymesmedicine.anatomical_structureCytokineCell cultureRANKLbiology.protein0210 nano-technologyJ. Cell. Biochem.
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Osteogenic differentiation of periodontal fibroblasts is dependent on the strength of mechanical strain

2012

Abstract Objective During orthodontic therapy the correct strength of mechanical strain plays a key role for bone remodelling during tooth movement. Aim of this study was to investigate the osteogenic differentiation of human periodontal ligament fibroblasts (HPdLF) depending on the applied strength of mechanical strain compared to osteoblasts (HOB). Design HPdLF and HOB were loaded with different strengths (1%, 5% and 10%) of static mechanical strain (SMS) for 12 h in vitro. Viability was verified by MTT and apoptosis by TUNEL assay. Gene expression of cyclin D1, collagen type-1 (COL-I), alkaline phosphatase (ALP), osteocalcin, osteoprotegerin (OPG) and receptor activator of the NF-κB liga…

musculoskeletal diseasesCell SurvivalPeriodontal LigamentGene ExpressionDentistryApoptosisEnzyme-Linked Immunosorbent AssayReal-Time Polymerase Chain ReactionCollagen Type IBone remodelingAndrologyCyclin D1OsteoprotegerinOsteogenesisIn Situ Nick-End LabelingHumansPeriodontal fiberCyclin D1RNA MessengerGeneral DentistryCells CulturedAnalysis of VarianceOsteoblastsTUNEL assaybiologybusiness.industryChemistryRANK LigandOsteoprotegerinCell DifferentiationCell BiologyGeneral MedicineFibroblastsAlkaline PhosphataseOtorhinolaryngologyRANKLOsteocalcinbiology.proteinAlkaline phosphataseStress MechanicalbusinessArchives of Oral Biology
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Hydration process and rheological properties of cement pastes modified by orthophosphate addition

2005

Abstract The use of cement by nuclear industry for confining low radioactive waste knows a constant increase. The interest bore to this material is due to its mechanical and chemical properties. However, these properties may be modified by the nature and the amount of wastes introduced with the mixing solution especially with large amount of phosphate. Precipitation of hydroxylapatite in the cement paste at the very beginning after mixing was emphasized from a particular concentration of orthophosphate. It was also found that this phenomenon leads to an early rigidification of the cement paste. From a kinetics point of view, the evolution of hydration was both delayed and slowed down accord…

musculoskeletal diseasesCementMaterials sciencePrecipitation (chemistry)Mixing (process engineering)MineralogyHydroxylapatitePhosphateApatitechemistry.chemical_compoundAdsorptionchemistryChemical engineeringRheologyvisual_artMaterials ChemistryCeramics and Compositesvisual_art.visual_art_mediumJournal of the European Ceramic Society
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Häufigkeit, Altersabhängigkeit und Geschlechtsverteilung des Fersensporns

1995

In 1027 lateral radiograms of the ankle in a Caucasian population, 161 plantar and/or dorsal calcaneal spurs (15.7%) were diagnosed. Plantar spurs were more common than dorsal spurs (11.2 and 9.3% respectively). Prevalence of both spurs increases considerably with the rising age. Dorsal spurs appear slightly earlier than plantar spurs. The spur frequencies are similar in left and right feet. The plantar spurs were significantly (p < 0.0001) more common in women than in men in general, while dorsal spurs were more frequent in men than in women up to the age of 70. The previously reported higher frequencies of plantar and dorsal calcaneal spurs in women than in men are probably a result of a …

musculoskeletal diseasesDorsumendocrine systembusiness.industryIncidence (epidemiology)AnatomyEuropean populationhumanitiesmedicine.anatomical_structurehemic and lymphatic diseasesparasitic diseasesSpurmedicineRadiology Nuclear Medicine and imagingAge distributionmedicine.symptomAnklebusinessCaucasian populationCalcaneal spurRöFo - Fortschritte auf dem Gebiet der Röntgenstrahlen und der bildgebenden Verfahren
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Expression of 60-kD Heat Shock Protein Increases during Carcinogenesis in the Uterine Exocervix

2002

&lt;i&gt;Objectives:&lt;/i&gt; The aim of the present study was to determine the presence and expression of the 60-kD heat shock protein (HSP60) in the dysplasia-carcinoma sequence in the uterine exocervix and to evaluate its diagnostic and prognostic significance. &lt;i&gt;Methods and Results:&lt;/i&gt; We performed Western blot and immunohistochemical analyses on biopsies from 40 cases, consisting of 10 normal exocervical biopsies, 10 low-grade squamous intraepithelial lesions (L-SIL), 10 high-grade squamous intraepithelial lesions (H-SIL) and 10 cancerous exocervices (G2 grade). The immunohistochemical results were quantified by computer-assisted image analysis. Western blot analysis sho…

musculoskeletal diseasesKoilocytePathologymedicine.medical_specialtyChaperonin; High-grade squamous intraepithelial lesion; Koilocyte; Low-grade squamous intraepithelial lesion; Squamous cervical cancer; 2734; Clinical Biochemistry; Immunology and Allergy; Cell BiologyBlotting Western2734Clinical BiochemistryUterine Cervical NeoplasmsBiologyChaperoninPathology and Forensic MedicineWestern blotimmune system diseasesLow-grade squamous intraepithelial lesionhemic and lymphatic diseasesHeat shock proteinImage Processing Computer-AssistedmedicineCarcinomaHumansImmunology and AllergyMolecular Biologymedicine.diagnostic_testChaperonin 60Cell BiologyGeneral MedicinePrognosisUterine Cervical Dysplasiamedicine.diseaseImmunohistochemistryMolecular biologyfemale genital diseases and pregnancy complicationsKoilocyteEpitheliumBlotmedicine.anatomical_structureHigh-grade squamous intraepithelial lesionCarcinoma Squamous CellImmunohistochemistryFemalePrecancerous ConditionsSquamous cervical cancerImmunostainingPathobiology
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Neridronate prevents bone loss in patients receiving androgen deprivation therapy for prostate cancer.

2004

Today, androgen deprivation therapy is a cornerstone of treatment for advanced prostate cancer, although it presents important complications such as osteoporosis. Neridronate, a relatively new bisphosphonate, is able to prevent bone loss in patients with prostate cancer during androgen ablation. Introduction: Androgen-deprivation therapy (ADT) is a cornerstone of treatment for advanced prostate cancer. This therapy has iatrogenic complications, such as osteoporosis. The aim of our study was to evaluate the efficacy of neridronate, a relatively new bisphosphonate, to prevent bone loss during androgen ablation. Materials and Methods: Forty-eight osteoporotic patients with prostate cancer, tre…

musculoskeletal diseasesMalemedicine.medical_specialtyDeoxypyridinolineTime FactorsBicalutamideAntineoplastic Agents HormonalEndocrinology Diabetes and Metabolismmedicine.medical_treatmentOsteoporosisUrologyBone and BonesAndrogen deprivation therapychemistry.chemical_compoundProstate cancerAbsorptiometry PhotonBone DensityMedicineNeridronic acidHumansOrthopedics and Sports MedicineAmino AcidsAgedCholecalciferolTriptorelin PamoateDiphosphonatesbusiness.industryProstatic NeoplasmsAndrogen AntagonistsBisphosphonatemedicine.diseaseAlkaline PhosphataseAndrogen deprivation therapy; Bisphosphonates; Neridronate; Osteoporosis; Prostate cancer; Absorptiometry Photon; Aged; Alkaline Phosphatase; Amino Acids; Androgen Antagonists; Androgens; Antineoplastic Agents Hormonal; Bone Density; Bone and Bones; Calcium; Cholecalciferol; Diphosphonates; Humans; Male; Osteoporosis; Prostatic Neoplasms; Time Factors; Triptorelin Pamoate; SurgerySurgerychemistryAndrogensOsteoporosisCalciumbusinessCholecalciferolmedicine.drugJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
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Lymphatic vessels of the dura mater: a new discovery?

2015

Aspelund et al. discover the presence of a lymphatic vessel network in the dura mater of the mouse brain and show that these dural lymphatic vessels are important for the clearance of macromolecules from the brain.

musculoskeletal diseasesPathologymedicine.medical_specialtyHistologyMacromolecular SubstancesDura materCentral nervous systemNeuroimmunologyBlood–brain barrierBlood-brain barrier; Brain; Central nervous system; Dura mater; Lymphatics; Mascagni; Neuroanatomy; Neuroimmunology; Transudate; Anatomy; Histology; Developmental Biology; Ecology Evolution Behavior and Systematics; Molecular Biology; Cell BiologyTransudateLymphatic SystemMatters ArisingmedicineAnimalsDura materMolecular BiologyEcology Evolution Behavior and SystematicsBlood-brain barrierCerebrospinal Fluidintegumentary systembusiness.industrySettore BIO/16 - Anatomia UmanaBrief Definitive ReportMascagniBrainExtracellular FluidAnatomyCell Biologymusculoskeletal systemEcology Evolution Behavior and SystematicTransudatenervous system diseasesNeuroanatomyNeuroimmunologymedicine.anatomical_structureLymphatic systemnervous systemCentral nervous systemLymph NodesAnatomybusinessLymphaticNeuroanatomyDevelopmental BiologyJournal of anatomy
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Bio-silica and bio-polyphosphate: applications in biomedicine (bone formation)

2012

Bio-silica represents the main mineral component of the sponge skeletal elements (siliceous spicules), while bio-polyphosphate (bio-polyP), a multifunctional polymer existing in microorganisms and animals acts, among others, as reinforcement for pores in cell membranes. These natural inorganic bio-polymers, which can be readily prepared, either by recombinant enzymes (bio-silica and bio-polyP) or chemically (polyP), are promising materials/substances for the amelioration and/or treatment of human bone diseases and dysfunctions. It has been demonstrated that bio-silica causes in vitro a differential effect on the expression of the genes OPG and RANKL, encoding two mediators that control the …

musculoskeletal diseasesSiliconAnabolismBiomedical EngineeringOsteoclastsBioengineering02 engineering and technologyBone morphogenetic protein 2Phosphates03 medical and health sciencesMediatorOsteogenesisAnimalsHumansProgenitor cell030304 developmental biology0303 health sciencesOsteoblastsbiologyCatabolismChemistry021001 nanoscience & nanotechnologySilicon DioxideIn vitro3. Good healthCell biologyPoriferaRANKLImmunologybiology.proteinOsteoporosisBone Diseases0210 nano-technologyFunction (biology)BiotechnologyCurr. Opin. Biotechnol.
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