Search results for "PHOSPHATE"

Sealing ability of five different retrograde filling materials.

1996

The sealing ability of Amalgam, Harvard-Cement, Diaket, gold-leaf, and Ketac-Endo as retrofilling materials was investigated. Paper cones were fixed with Harvard-Cement in the instrumented roots of 100 extracted human incisors. Apicectomy was performed and a 2-mm-deep retrograde cavity was prepared. Teeth were assigned to five groups (n = 20); each group received a different filling material. Surfaces of the roots were isolated with nail polish. Teeth, were stored in 1% methylene blue dye for 72 h. Roots were sectioned, and the depth of dye penetration was evaluated through a stereomicroscope. Retrofills with Ketac-Endo showed significantly less leakage compared with amalgam. There was no s…

Loss of endocytic clathrin-coated pits upon acute depletion of phosphatidylinositol 4,5-bisphosphate.

2007

Phosphatidylinositol 4,5-bisphosphate [PI(4,5) P 2 ], a phosphoinositide concentrated predominantly in the plasma membrane, binds endocytic clathrin adaptors, many of their accessory factors, and a variety of actin-regulatory proteins. Here we have used fluorescent fusion proteins and total internal reflection fluorescence microscopy to investigate the effect of acute PI(4,5) P 2 breakdown on the dynamics of endocytic clathrin-coated pit components and of the actin regulatory complex, Arp2/3. PI(4,5) P 2 breakdown was achieved by the inducible recruitment to the plasma membrane of an inositol 5-phosphatase module through the rapamycin/FRB/FKBP system or by treatment with ionomycin. PI(4,5)…

Sequence variation and regulatory variation in acetylcholinesterase genes contribute to insecticide resistance in different populations of Leptinotar…

2021

Abstract Although insect herbivores are known to evolve resistance to insecticides through multiple genetic mechanisms, resistance in individual species has been assumed to follow the same mechanism. While both mutations in the target site insensitivity and increased amplification are known to contribute to insecticide resistance, little is known about the degree to which geographic populations of the same species differ at the target site in a response to insecticides. We tested structural (e.g., mutation profiles) and regulatory (e.g., the gene expression of Ldace1 and Ldace2, AChE activity) differences between two populations (Vermont, USA and Belchow, Poland) of the Colorado potato beet…

Peculiarities of Phase Formation in Mn-Based Na SuperIonic Conductor (NaSICon) Systems: The Case of Na1+2xMnxTi2–x(PO4)3 (0.0 ≤ x ≤ 1.5)

2021

This project has received funding from the European Regional Development Fund (Project no. 01.2.2-LMT-K-718-02–0005) under grant agreement with the Research Council of Lithuania (LMTLT). We thank the High Performance Computing Center “HPC Saulėtekis” at the Faculty of Physics, Vilnius University, for the use of computational resources.

Fragmentations and reactions of some isotopically labelled dimethyl methyl phosphono and trimethyl phosphoro thiolates and thionates studied by elect…

2005

In this paper, studies on electrospray ionisation ion trap mass spectrometry of organophosphates are extended to a series of dimethyl methylphosphono and trimethyl phosphoro thionates and thiolates and some deuterated isotopomers. Of particular, interest is the comparison of the collision-induced fragmentation of ions from these compounds with those of the non-sulphur containing analogues reported previously. The thiono and thiolo isomeric structures of the sulphur containing ions analogous to I and II (see below) have very similar energies and undergo a ready interconversion. In the case of the phosphono compounds, the electronic structure calculations show that the methyl migration implic…

The Daily Consumption of Cola Can Determine Hypocalcemia: A Case Report of Postsurgical Hypoparathyroidism-Related Hypocalcemia Refractory to Supplem…

2017

The consumption of soft drinks is a crucial factor in determining persistent hypocalcemia. The aim of the study is to evaluate the biochemical mechanisms inducing hypocalcemia in a female patient with usual high consumption of cola drink and persistent hypocalcemia, who failed to respond to high doses of calcium and calcitriol supplementation. At baseline and after pentagastrin injection, gastric secretion (Gs) and duodenal secretion (Ds) samples were collected and calcium and total phosphorus (Ptot) concentrations were evaluated. At the same time, blood calcium, Ptot, sodium, potassium, chloride, magnesium concentrations, and vitamin D were sampled. After intake of cola (1 L) over 180 min,…

Fabrication of cobalt and cobalt oxide/graphene composites: towards high-performance anode materials for lithium ion batteries.

2010

Application of a fuzzy algorithm for pH control in a struvite crystallisation reactor

2006

A struvite crystallisation process is highly dependent on pH. To achieve a high phosphorus recovery as struvite it is important to have an accurate control of pH in the reactor. The high non-linear response of pH value makes manual pH control difficult. Therefore, a software based on fuzzy logic control (FLC) has been developed to maintain the pH at a set value in a stirred reactor to crystallise struvite. The FLC developed has been based on Larsen's inference. In order to confirm the improvement of the pH stability using FLC software, different experiments have been carried out with manual control of the pH value, and with the FLC software. It has been demonstrated that using FLC software …

Modelling the metabolic shift of polyphosphate-accumulating organisms

2014

Enhanced biological phosphorus removal (EBPR) is one of the most important methods of phosphorus removal in municipal wastewater treatment plants, having been described by different modelling approaches. In this process, the PAOs (polyphosphate accumulating organisms) and GAOs (glycogen accumulating organisms) compete for volatile fatty acids uptake under anaerobic conditions. Recent studies have revealed that the metabolic pathways used by PAOs in order to obtain the energy and the reducing power needed for polyhydroxyalkanoates synthesis could change depending on the amount of polyphosphate stored in the cells. The model presented in this paper extends beyond previously developed metaboli…

Optimizing the H3PO4 leaching conditions of post-precipitated sewage sludge using response surface methodology

2018

The leaching procedure of post-precipitated aluminium phosphate sludge with dilute phosphoric acid was developed. The leaching offers a route to recover both critical phosphorous from sewage sludge and the metal used in precipitation. Using phosphoric acid as leaching solution makes it possible to continue the recovery process without the need to remove chloride or sulfate anions. The optimization of the leaching was based on experimental three-level-four-variable central composite face-centered design. The four variables included were acid concentration, volume of acid, temperature and time of leaching. The leaching was conducted for dewatered and water-containing sludge (total solid conte…