Search results for "PLANT"
showing 10 items of 11944 documents
Single-chain antibodies against a plant viral RNA-dependent RNA polymerase confer virus resistance.
2004
Crop loss due to viral diseases is still a major problem for agriculture today. We present a strategy to achieve virus resistance based on the expression of single-chain Fv fragments (scFvs) against a conserved domain in a plant viral RNA-dependent RNA polymerase (RdRp), a key enzyme in virus replication. The selected scFvs inhibited complementary RNA synthesis of different plant virus RdRps in vitro and virus replication in planta. Moreover, the scFvs also bound to the RdRp of the distantly related hepatitis C virus. T(1) and T(2) progeny of transgenic lines of Nicotiana benthamiana expressing different scFvs either in the cytosol or in the endoplasmic reticulum showed varying degrees of r…
Oncolytic targeting of renal cell carcinoma via encephalomyocarditis virus
2010
Apoptosis is a fundamental host defence mechanism against invading microbes. Inactivation of NF-kappaB attenuates encephalomyocarditis virus (EMCV) virulence by triggering rapid apoptosis of infected cells, thereby pre-emptively limiting viral replication. Recent evidence has shown that hypoxia-inducible factor (HIF) increases NF-kappaB-mediated anti-apoptotic response in clear-cell renal cell carcinoma (CCRCC) that commonly exhibit hyperactivation of HIF due to the loss of its principal negative regulator, von Hippel-Lindau (VHL) tumour suppressor protein. Here, we show that EMCV challenge induces a strong NF-kappaB-dependent gene expression profile concomitant with a lack of interferon-me…
Quantitative microscopy reveals stepwise alteration of chromatin structure during herpesvirus infection
2019
During lytic herpes simplex virus 1 (HSV-1) infection, the expansion of the viral replication compartments leads to an enrichment of the host chromatin in the peripheral nucleoplasm. We have shown previously that HSV-1 infection induces the formation of channels through the compacted peripheral chromatin. Here, we used three-dimensional confocal and expansion microscopy, soft X-ray tomography, electron microscopy, and random walk simulations to analyze the kinetics of host chromatin redistribution and capsid localization relative to their egress site at the nuclear envelope. Our data demonstrated a gradual increase in chromatin marginalization, and the kinetics of chromatin smoothening arou…
Antiviral prophylaxis in patients with haematological malignancies and solid tumours: Guidelines of the Infectious Diseases Working Party (AGIHO) of …
2006
Morbidity and mortality in patients with malignancies are increased by viral infections. These mostly are reactivations of asymptomatic latent infections. They primarily concern clinical entities associated with the reactivation of herpes viruses, such as varicella zoster virus (VZV) and cytomegalovirus (CMV). Respiratory tract infections caused by influenza, parainfluenza or respiratory syncytial virus (RSV) are less common. Since reactivation of latent infections has major clinical impact, antiviral prophylaxis is an attractive approach for patients expecting immunosuppression. The main risk factor for clinically relevant reactivation is profound disruption of cellular immune response. Du…
A single tube PCR assay for detecting viruses and their recombinants that cause tomato yellow leaf curl disease in Mediterranean basin
2007
Tomato yellow leaf curl disease (TYLCD) is well known in Mediterranean countries, where it has been causing severe losses in tomato crops for decades. Until recently, two viruses (with several isolates) in the genus Begomovirus, family Geminiviridae, have been associated with the epidemics: Tomato yellow leaf curl virus (TYLCV) and Tomato yellow leaf curl Sardinia virus (TYLCSV). However, recombinants between these, such as Tomato yellow leaf curl Malaga virus (TYLCMalV), are spreading, and new methods for detecting all viruses present in the region are needed. By considering all DNA sequences available of viruses causing TYLCD in the Mediterranean basin, a PCR/RFLP protocol was developed t…
Pēcoperācijas perioda izvērtējums pēc radzenes transplantācijas vispārējā anestēzijā
2020
Bakalaura darba tēma ir “Pēcoperācijas perioda izvērtējums pēc radzenes transplantācijas vispārējā anestēzijā”. Darba tēmas aktualitāte ir tajā, ka radzenes transplantācijas operācijas visā pasaulē un Latvijā tiek veiktas arvien vairāk, tāpēc ir būtiski novērtēt pēcoperācijas periodu pēc šīm operācijām, lai izvērtēt aprūpes procesu, pacientu sūdzības un dokumentācijas kvalitāti un nepieciešamības gadījumā veikt uzlabojums. Darba mērķis ir izvērtēt pēcoperācijas periodu pacientam pēc radzenes transplantācijas operācijas vispārējā anestēzijā. Bakalaura darba mērķa saniegšanai autore izvirzija sekojošus darba uzdevumus - apkopot un analizēt zinātnisko literatūru par radzenes transplantāciju un…
Endophytic Fungi in Asymptomatic Vitis vinifera L. and their Effects on Plasmopara viticola
2016
During a multi-year study on the life cycle of Plasmopara viticola to assess the typical epidemiology of downy mildew on grapevine (Vitis vinifera) in west Sicily (Italy), several leaves on which the downy mildew had collapsed were found and observed in a vineyard located in Marsala, in Trapani Province. An antagonistic fungus was isolated from these leaves and named A20; through its morphology, it was identified as a hyphomycete belonging to the genus Acremonium. The antagonism of A20 against the asexual and sexual spores of P. viticola, as well as endophytic nature of the genus Acremonium in V. vinifera were confirmed by further studies which have been carried out over the past decade. In…
The recent Nyiragongo (Democratic Republic of Congo) eruption: the impact of volcanic ash fallout on drinking water and edible plants
2022
Nyiragongo is an active intraplate volcano well known for its fascinating persistent lava lake inside the crater and is recognized as one of the most dangerous volcanoes in the world as more than two million people live on its slopes, 18-25 km far from the main crater. It is located in the Virunga Volcanic Province (VVP), in the western branch of the East African Rift System (EARS), at the intersection between the Democratic Republic of Congo, Rwanda, and Uganda. Unexpectedly, on 22 May 2021, Nyiragongo produced three different lateral lava flows in the low flanks and significant amounts of volcanic gas and ash were emitted from the summit crater. For several weeks, the ash fallout strongly…
Complex plant quality—microbiota–population interactions modulate the response of a specialist herbivore to the defence of its host plant
2022
Many specialist herbivores have evolved strategies to cope with plant defences, with gut microbiota potentially participating to such adaptations. In this study, we assessed whether the history of plant use (population origin) and microbiota may interact with plant defence adaptation. We tested whether microbiota enhance the performance of Melitaea cinxia larvae on their host plant, Plantago lanceolata and increase their ability to cope the defensive compounds, iridoid glycosides (IGs). The gut microbiota were significantly affected by both larval population origin and host plant IG level. Contrary to our prediction, impoverishing the microbiota with antibiotic treatment did not reduce larv…
Foot loading in normal and pathological walking
2002
Perusteellinen kävely- ja kuormitusanalyysi on usein tarpeellinen jalkaongelmissa, jotta mahdollinen tukien ja ortoosien valinta sekä alaraajoihin kohdistuvien leikkausten jälkeinen kuntoutus pystytään suorittamaan parhaalla mahdollisella tavalla, toteaa Jarmo Perttunen väitöstutkimuksessaan. Kävelijä tuottaa tukivaiheen aikana alustaan voimia pysty- ja vaakasuunnassa, joita on perinteisesti mitattu voimalevyantureilla. Ne eivät pysty yksistään erottelemaan kuormitusta jalkapohjan eri osien välillä riittävän täsmällisesti. Kiinnostus selvittää kuormitus tarkasti on johtanut kehityksen alkeellisista jalkapohjan mustekuvista hyvin pieniin kengän sisälle asetettaviin mittausantureihin. Perttus…