Search results for "PLASMA"
showing 10 items of 4043 documents
CD28, a marker associated with tumoral expansion in multiple myeloma
1998
International audience; CD28 expression was thoroughly investigated on plasma cells of monoclonal gammopathy of undetermined significance, multiple myeloma (MM), and human myeloma cell lines. CD28+ plasma cells were detected in 19% of 31 monoclonal gammopathy of undetermined significance, 41% of 116 MM, and 100% of 13 human myeloma cell lines. CD28+ myeloma cells were detected in 21 of 79 (26%) MM cases at diagnosis, 13 of 22 (59%) at medullary relapse (P < 0.009), and 14 of 15 (93%) at extramedullary relapse (P = 0.05), including 10 of 10 (100%) secondary plasma cell leukemias (P = 0.05). Serial studies in individual patients confirmed the emergence of CD28+ myeloma cells with tumoral expa…
Dynamical and statistical properties of high-temperature self-propagating fronts: An experimental study
2009
International audience; We present a detailed experimental study of high-temperature self-propagating fronts using image processing techniques. The intrinsic features of the wave propagation are investigated as a function of the combustion temperature TC for a model system made of titanium and silicon powders. Different front behavior is realized by changing the molar ratio x of the mixture Ti+xSi. Outside the range x=[0.3,1.5], no thermal front is propagating while inside, three regimes are observed: steady-state combustion which is characterized by a flat front propagating at constant velocity and two unsteady regimes. The combustion temperature (or the corresponding ratio x) is thus play…
Solidification microstructure during selective laser melting of Ni based superalloy: experiment and mesoscopic modelling
2019
International audience; A set of single track laser melting experiments was performed in a selective laser melting (SLM). The tracks were done on an Inconel 718 plate with various laser scan velocities at a constant laser power of 150 W. The geometries of the molten pool (MP), as well as the solidified dendrite structures, i.e., primary and secondary dendrite arm spacing (PDAS and SDAS), in the cross sections of the molten path were characterized to evaluate the effect of the laser scan velocity during SLM. Moreover, the local solidification thermal conditions (cooling rate R*, tip growth velocity V* and temperature gradient G*) at the MP bottom were deduced from the SDAS and the geometries…
Plasmatic extracellular vesicle microRNAs in malignant pleural mesothelioma and asbestos-exposed subjects suggest a 2-miRNA signature as potential bi…
2017
Background Malignant Pleural Mesothelioma (MPM) is an aggressive cancer mainly caused by asbestos exposure and refractory to current therapies. Specific diagnostic markers for early MPM diagnosis are needed. Changes in miRNA expression have been implicated in several diseases and cancers, including MPM. We examined if a specific miRNA signature in plasmatic extracellular vesicles (EV) may help to discriminate between malignant pleural mesothelioma patients (MPM) and subjects with Past Asbestos Exposure (PAE). Methodology/Principal findings We investigated 23 MPM patients and 19 cancer-free subjects with past asbestos exposure (PAE). We screened 754 miRNAs in plasmatic EVs by OpenArray and f…
Relationships of Plasma Aldosterone with Metabolic Syndrome and Left Ventricular Mass in Essential Hypertensive Patients
2008
TALE ARTICOLO NON HA ABSTRACT
Phenolic and microbial-targeted metabolomics to discovering and evaluating wine intake biomarkers in human urine and plasma
2014
The discovery of biomarkers of intake in nutritional epidemiological studies is essential in establishing an association between dietary intake (considering their bioavailability) and diet-related risk factors for diseases. The aim is to study urine and plasma phenolic and microbial profile by targeted metabolomics approach in a wine intervention clinical trial for discovering and evaluating food intake biomarkers. High-risk male volunteers (n = 36) were included in a randomized, crossover intervention clinical trial. After a washout period, subjects received red wine or gin, or dealcoholized red wine over four weeks. Fasting plasma and 24-h urine were collected at baseline and after each i…
Metabolite and mineral profiling of “Violetto di Niscemi” and “Spinoso di Menfi” globe artichokes by 1H-NMR and ICP-MS
2016
Globe artichoke has been long considered a nutraceutical food for its valuable content of bioactive compounds. However, beside a well-known polyphenol profile, poor information is available about its metabolite and mineral composition. The aim of this study was to investigate edible parts of Sicilian artichokes, ‘Spinoso di Menfi’ and ‘Violetto di Niscemi’, by 1H NMR and ICP-MS for elucidating these compositional aspects. Although bracts and hearts of both artichokes shared a very similar metabolite pattern, ‘Spinoso di Menfi’ showed a higher number of metabolites, such as amino acids and polyphenols, than ‘Violetto di Niscemi’. ‘Spinoso di Menfi’ was also marked by higher levels of macro- …
Can the plasma PD-1 levels predict the presence and efficiency of tumor-infiltrating lymphocytes in patients with metastatic melanoma?
2019
Background: The immune response in melanoma patients is locally affected by presence of tumor-infiltrating lymphocytes (TILs), generally divided into brisk, nonbrisk, and absent. Several studies have shown that a greater presence of TILs, especially brisk, in primary melanoma is associated with a better prognosis and higher survival rate. Patients and Methods: We investigated by enzyme-linked immunosorbent assay (ELISA) the correlation between PD-1 levels in plasma and the presence/absence of TILs in 28 patients with metastatic melanoma. Results: Low plasma PD-1 levels were correlated with brisk TILs in primary melanoma, whereas intermediate values correlated with the nonbrisk TILs, and hig…
Effect of sludge age on microbial consortia developed in MFCs
2017
BACKGROUND This work is focused on the assessment of the performance of mini-scale air-breathing microbial fuel cells (MFCs), by monitoring the evolution of the bio-electrogenic activity for a period of 40 days and by comparing the microorganisms populations developed in each of the MFC after this period. RESULTS Five MFCs were operated at sludge ages ranging from 1.4 to 10.0 days. Results showed the superb performance of the MFC operating under a sludge age of 2.5 days. Desulfuromonas, Syntrophothermus, Solitalea, Acholeplasma, Propionicimonas, Desulfobacula and Sphaerochaeta are proposed as potential responsible for the bio-electrogenic activity. CONCLUSIONS Microbial population analysis …
Rapid isolation of single microbial cells from mixed natural and laboratory populations with the aid of a micromanipulator.
1999
In order to facilitate the isolation of pure cultures from natural habitats we have developed a method for the isolation of single microbial cell clones from a mixed population, e.g. the flora of the termite gut, with the aid of a modern micromanipulator. The separated single prokaryotic or eukaryotic cells were grown after transfer in culture media or they were used for single cell PCR. The micromanipulator was also applied for the removal of nuclei from protozoa, of which the SSU rDNA was directly amplified.