Search results for "PLE"
showing 10 items of 22773 documents
Multiplex Ligation-dependent Probe Amplification (MLPA)
2011
The Multiplex Ligation-dependent Probe Amplification (MLPA) is a PCR-based method. The procedure relies on sequence-specific probe hybridization of genomic DNA, followed by multiplex-PCR amplification of the hybridized probe and a semiquantitative analysis of the resulting PCR products. MLPA allows the analysis of around 40 loci in the same reaction, and is a sensitive and relatively fast technique. Only a small amount of DNA is required and results are available within 2 days.The critical factors when performing MLPA analyses from formalin-fixed paraffin-embedded (FFPE) tissues are DNA integrity and purity; for this reason, a suitable DNA extraction method must be chosen.The MLPA protocol …
Hip viscosupplementation under ultra-sound guidance riduces NSAID consumption in symptomatic hip osteoarthritis patients in a long follow-up. Data fr…
2011
Introduction: Non-steroidal anti-inflammatory drugs (NSAIDs) consumption is strictly related to a high gastrointestinal and cardiovascular mortality and morbidity rate. Osteoarthritis Research Society International (OARSI) recommendations in patients with symptomatic hip or knee OA stated that NSAIDs should be used at the lowest effective dose but their long-term use should be avoided if possible. OARSI guidelines for the treatment of the hip OA include the use of viscosupplementation, which aims to restore physiological and rheological features of the synovial fluid. Objective: Aim of this multicentric, open and retrospective study is to investigate if NSAID consumption may be reduced by t…
Acupuncture in the treatment of temporomandibular disorders, facial pain, and postural neuromuscular disorders. Presentation of a clinical case
2021
Abstract Background and objectives Temporomandibular disorders have a multifactorial etiology and involve the masticatory muscles, temporomandibular joint and associated structures. Therefore, different treatments are proposed and used. The aim of this paper is the use of acupuncture to treat temporomandibular disorders and associated symptoms. This article presents a clinical case study of a 39-year-old patient who presented temporomandibular disorders and headache of muscular origin. Results After 10 acupuncture sessions, the results were highly satisfactory, with complete relief of headache pain and absence of joint disorders. These results suggest the efficacy of acupuncture in the symp…
Changes in spinal range of motion after a flexibility training program in elderly women
2014
Giuseppe Battaglia,1,2 Marianna Bellafiore,1,2 Giovanni Caramazza,2 Antonio Paoli,3 Antonino Bianco,1,2 Antonio Palma1,2 1Department of Law, Society, and Sport Sciences, University of Palermo, Palermo, Italy; 2Sicilian Regional Sports School of Italian National Olympic Committee (CONI), Sicily, Italy; 3Department of Biomedical Sciences, University of Padova, Padova, Italy Background: Aging-related reduced spinal mobility can interfere with the execution of important functional skills and activities in elderly women. Although several studies have shown positive outcomes in response to spinal flexibility training programs, little is known about the management of sets and repetitions in traini…
Coronary Stent Strut Fractures: Classification, Prevalence and Clinical Associations
2021
Introduction. The frequency, characteristics and clinical implications of Strut fractures (SFs) remain incompletely understood. Methods and results. A total of 185 (160 patients) newer-generation drug-eluting stents (DES) were imaged. SFs were found in 21 DES (11.4%) and were classified in four patterns: one single stacked strut (41%)
Rhythmic exercises as tools for rehabilitation following cerebellar stroke: A case study integrating music therapy and physiotherapy techniques
2022
Introduction: This article explores the use of music and multisensory stimuli in the construction of compensatory neural networks for motoric functioning in a patient recovering from cerebellar strokes (CS). This study aimed to address the real-world clinical concern of patients having a passive role in therapy, by arousing the client’s interest and self-motivation in rehabilitation. Method: The article presents a case study of a CS survivor, using data derived from rehabilitation sessions combining music therapy techniques with physiotherapy techniques to improve the fluency and accuracy of his motor performance. Qualitative and quantitative data are used to identify, describe, and evaluat…
Evaluating the Consonance and Pleasantness of Triads in Different Musical Context
2013
This study examines whether the consonance and pleasantness of triads (major, minor, augmented, and diminished) varies according to the musical context in which it is presented. The level of consonance and dissonance (C/D) of each chord when they were played alone without any musical contexts was judged. Following this, each chord was accommodated in a different position in a short cadence, and the level of C/D for each chord was judged. Additionally, the C/D and the pleasantness and unpleasantness (P/U) of the whole sequence were rated on a 7-point scale. The results show that, for major and minor triads, there was no significant difference in C/D levels between the ‘without musical contex…
Complete Vocal Technique laulunopetuksen välineenä lukion musiikinopetuksessa
2015
Tutkimuksen tarkoituksena on selvittää miten Complete Vocal Technique (CVT) nimistä tanskalaista laulutekniikkaa voidaan hyödyntää lukion musiikinopetuksen laulunopetuksessa. Kyseistä laulutekniikkaa käytetään niin pop/jazz-laulun, kuin klassisen laulunkin opetuksessa. Se on selkeä, yksinkertainen ja monenlaiset oppijat huomioon ottava laulutekniikka, jonka avulla opiskelijat voivat oppia ymmärtämään laulamista ja rohkaistua käyttämään omaa ääntään. Tutkimus on toimintatutkimus. Pidin kolme Complete Vocal Techniqueen liittyvää laulutyöpajaa yhdelle musiikin 1. kurssin lukiolaisryhmälle. Koska tutkimuksessa oli mukana vain yksi lukiolaisryhmä, on tutkimus myös tapaustutkimus. Työpajojen jälk…
Selective hemicellulose hydrolysis of Scots pine sawdust
2019
The depletion of fossil resources is driving forward the search for new and alternative renewable feedstocks in the production of renewable chemicals, which could replace the petroleum-based ones. One such feedstock is pine (Pinus sylvestris) sawdust, which is generated enormous amounts in Finnish sawmills yearly. However, prior to the utilization in high-value applications, it needs to be fractionated into its constituents. In this work, the objective was to produce monomeric hemicellulose sugars from pine sawdust without degrading cellulose or lignin simultaneously. The influence of the reaction temperature and time, as well as acid type and concentration, was studied. Based on the result…
Management of Statin Intolerance in 2018: Still More Questions Than Answers.
2018
Statin therapy is generally well tolerated and very effective in the prevention and treatment of cardiovascular disease, regardless of cholesterol levels; however, it can be associated with various adverse events (myalgia, myopathy, rhabdomyolysis, and diabetes mellitus, among others). Patients frequently discontinue statin therapy without medical advice because of perceived side effects and consequently increase their risk for cardiovascular events. In patients with statin intolerance, it may be advisable to change the dose, switch to a different statin, or try an alternate-day regimen. If intolerance is associated with all statins—even at the lowest dose—non-statin drugs and certain nutra…