Search results for "PLE"

showing 10 items of 22773 documents

Thioredoxin (Trxo1) interacts with proliferating cell nuclear antigen (PCNA) and its overexpression affects the growth of tobacco cell culture.

2017

Thioredoxins (Trxs), key components of cellular redox regulation, act by controlling the redox status of many target proteins, and have been shown to play an essential role in cell survival and growth. The presence of a Trx system in the nucleus has received little attention in plants, and the nuclear targets of plant Trxs have not been conclusively identified. Thus, very little is known about the function of Trxs in this cellular compartment. Previously, we studied the intracellular localization of PsTrxo1 and confirmed its presence in mitochondria and, interestingly, in the nucleus under standard growth conditions. In investigating the nuclear function of PsTrxo1 we identified proliferati…

0106 biological sciences0301 basic medicineTFs transcription factorsOverexpressionBiologíaBiFC bimolecular fluorescence complementationClinical BiochemistryCell Culture TechniquesTobacco BY-2 cells01 natural sciencesBiochemistryTBY-2 tobacco bright yellow-2DTT 14-dithiothreitolBimolecular fluorescence complementationThioredoxinsGene Expression Regulation PlantTrx thioredoxinlcsh:QH301-705.5GFP green fluorescent proteinlcsh:R5-920biologyProliferating cell nuclear antigen (PCNA)Cell cycleGlutathione3. Good healthCell biologyMitochondriaNTR NADPH thioredoxin reductaseProtein TransportDEM diethyl maleateRT-qPCR Reverse transcription quantitative polymerase chain reactionThioredoxinlcsh:Medicine (General)Oxidation-ReductionAMS 4-acetamido-4-maleimidylstilbene-22-disulfonic acidResearch PaperPCNA proliferating cell nuclear antigenOex overexpressingCell cycleNucleusThioredoxin o103 medical and health sciencesROS reactive oxygen speciesDownregulation and upregulationProliferating Cell Nuclear AntigenTobaccoDAPI 46-diamidine-2-phenylindolmCBM monochlorobimaneCellular compartmentCell NucleusCell growthOrganic ChemistryBotánicaPeasMolecular biologyYFP yellow fluorescent proteinProliferating cell nuclear antigenTBS Tris-buffered salineOD optical density030104 developmental biologylcsh:Biology (General)Cell cultureRNA reactive nitrogen speciesbiology.proteinPrx peroxiredoxinBSA bovine serum albumin010606 plant biology & botanyRedox biology
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Marine Cryptophytes Are Great Sources of EPA and DHA

2017

Microalgae have the ability to synthetize many compounds, some of which have been recognized as a source of functional ingredients for nutraceuticals with positive health effects. One well-known example is the long-chain polyunsaturated fatty acids (PUFAs), which are essential for human nutrition. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are the two most important long-chain omega-3 (-3) PUFAs involved in human physiology, and both industries are almost exclusively based on microalgae. In addition, algae produce phytosterols that reduce serum cholesterol. Here we determined the growth rates, biomass yields, PUFA and sterol content, and daily gain of eight strains of marine…

0106 biological sciences0301 basic medicineTROPICAL AUSTRALIAN MICROALGAELINOLENIC ACIDrasvahapotPharmaceutical Sciencesterols01 natural scienceschemistry.chemical_compoundFunctional FoodDrug DiscoveryFood scienceBiomasslcsh:QH301-705.5Pharmacology Toxicology and Pharmaceutics (miscellaneous)functional foodsPOLYUNSATURATED FATTY-ACIDSchemistry.chemical_classificationnutraceuticalsFRESH-WATERPRODUCTIVITYbiologymicroalgae; polyunsaturated fatty acids; omega-3; omega-6; sterols; functional foods; nutraceuticalsCHOLESTEROLmicroalgaeNANNOCHLOROPSISPhytosterolsfood and beveragesEicosapentaenoic acidEicosapentaenoic AcidDocosahexaenoic acidFatty Acids Unsaturatedlipids (amino acids peptides and proteins)omega-3CryptophytaPolyunsaturated fatty acidpolyunsaturated fatty acidsDocosahexaenoic Acidsomega-6CHEMICAL-COMPOSITIONterveysvaikutteiset elintarvikkeetfatty acidsGas Chromatography-Mass SpectrometryArticleMARICULTURE03 medical and health sciencesNutraceuticalAlgaeFatty Acids Omega-6Fatty Acids Omega-31172 Environmental sciencessterolit010604 marine biology & hydrobiologyomega fatty acidsta1183ta1182GROWTH-RATEmikrolevätbiology.organism_classificationSterolomegarasvahapot030104 developmental biologyHuman nutrition416 Food Sciencelcsh:Biology (General)chemistry13. Climate actionDietary SupplementsStearidonic acidMarine Drugs; Volume 16; Issue 1; Pages: 3
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UV-screening and springtime recovery of photosynthetic capacity in leaves of Vaccinium vitis-idaea above and below the snow pack

2019

International audience; Evergreen plants in boreal biomes undergo seasonal hardening and dehardening adjusting their photosynthetic capacity and photoprotection; acclimating to seasonal changes in temperature and irradiance. Leaf epidermal ultraviolet (UV)-screening by flavonols responds to solar radiation, perceived in part through increased ultraviolet-B (UV-B) radiation, and is a candidate trait to provide cross-photoprotection. At Hyytiälä Forestry Station, central Finland, we examined whether the accumulation of flavonols was higher in leaves of Vaccinium vitis-idaea L. growing above the snowpack compared with those below the snowpack. We found that leaves exposed to colder temperature…

0106 biological sciences0301 basic medicineTime FactorsPhotoinhibitionBOREALPhysiologyPlant ScienceForests01 natural sciencesPlant EpidermisAnthocyaninsSoilFlavonolsLOW-TEMPERATURESnowPhotosynthesis1183 Plant biology microbiology virologychemistry.chemical_classificationspring dehardening.CLIMATE-CHANGEbiologyChemistryTemperatureUnderstoreyHorticultureLIGHTSeasonsVacciniumUltraviolet RaysGrowing seasonPhotosynthesisDWARF SHRUB03 medical and health sciencesLEAFPHOTOSYSTEM-IIGenetics[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyVaccinium vitis-idaeaFlavonoidsSpring dehardeningPhotoprotectionSpectral qualityPhotosystem II Protein ComplexPigments Biological15. Life on landEvergreenbiology.organism_classificationPhotosynthetic capacitySUB-ARCTIC HEATHPlant Leaves030104 developmental biology13. Climate actionPhotoprotectionWINTERB RADIATIONArctic browning010606 plant biology & botany
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Gut microbiota of the pine weevil degrades conifer diterpenes and increases insect fitness

2017

AbstractThe pine weevil (Hylobius abietis), a major pest of conifer forests throughout Europe, feeds on the bark and cambium, tissues rich in terpenoid resins that are toxic to many insect herbivores. Here we report the ability of the pine weevil gut microbiota to degrade the diterpene acids of Norway spruce. The diterpene acid levels present in ingested bark were substantially reduced on passage through the pine weevil gut. This reduction was significantly less upon antibiotic treatment, and supplementing the diet with gut suspensions from untreated insects restored the ability to degrade diterpenes. In addition, cultured bacteria isolated from pine weevil guts were shown to degrade a Norw…

0106 biological sciences0301 basic medicineZoologyGut floracomplex mixtures010603 evolutionary biology01 natural sciences03 medical and health scienceschemistry.chemical_compoundSymbiosisBotanyGeneticsAnimalsHylobius abietisPiceaEcology Evolution Behavior and SystematicsbiologyHost (biology)Weevilfungibiology.organism_classificationGastrointestinal MicrobiomeEurope030104 developmental biologychemistryvisual_artvisual_art.visual_art_mediumWeevilsBarkGenetic FitnessDiterpenesDiterpeneBacteriaMolecular Ecology
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Plant Responses to Abiotic Stress Regulated by Histone Deacetylases

2017

In eukaryotic cells, histone acetylation and deacetylation play an important role in the regulation of gene expression. Histone acetylation levels are modulated by histone acetyltransferases (HATs) and histone deacetylases (HDACs). Recent studies indicate that HDACs play essential roles in the regulation of gene expression in plant response to environmental stress. In this review, we discussed the recent advance regarding the plant HDACs and their functions in the regulation of abiotic stress responses. The role of HDACs in autophagy was also discussed.

0106 biological sciences0301 basic medicineautophagyabiotic stressHistone acetylation and deacetylationMini ReviewPlant Sciencelcsh:Plant culture01 natural sciencesEnvironmental stress03 medical and health scienceschemistry.chemical_compoundhistone deacetylationlcsh:SB1-1110Histone AcetyltransferasesRegulation of gene expressionprotein complexesbiologyAbiotic stressAutophagyHDACsCell biology030104 developmental biologyHistonechemistryAcetylationbiology.protein010606 plant biology & botanyFrontiers in Plant Science
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On the origin of the recent herpetofauna of Sicily: Comparative phylogeography using homologous mitochondrial and nuclear genes

2016

Abstract In contrast to oceanic, continental islands are expected to show less diversification and endemism and thus phylogeographic signatures of multiple colonization events from adjacent continents due to episodic connections by sea level changes. In order to test this situation for the herpetofauna of Sicily, we here focus on three amphibian and four reptile species-groups and investigate their phylogeographic relationships across the Sicily and Messina straits, where Plio-Pleistocene marine transgressions shortened the distances between (or connected) Sicily, North Africa and/or the Italian (Apennine) Peninsula. Using a multi-species, multi-marker phylogeographic approach (mitochondria…

0106 biological sciences0301 basic medicinebiologyPleistoceneInsular biogeographyEcologybiology.organism_classification010603 evolutionary biology01 natural sciencesTarentola mauritanicalanguage.human_language03 medical and health sciencesPhylogeography030104 developmental biologyChalcides chalcideslanguageAnimal Science and ZoologyEndemismChalcides ocellatusSicilianZoologischer Anzeiger - A Journal of Comparative Zoology
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Alternative Splicing of the Basic Helix–Loop–Helix Transcription Factor Gene CmbHLH2 Affects Anthocyanin Biosynthesis in Ray Florets of Chrysanthemum…

2021

Chrysanthemum is an important ornamental crop worldwide. Some white-flowered chrysanthemum cultivars produce red ray florets under natural cultivation conditions, but little is known about how this occurs. We compared the expression of anthocyanin biosynthetic and transcription factor genes between white ray florets and those that turned red based on cultivation conditions to comprehend the underlying mechanism. Significant differences in the expression of CmbHLH2 were detected between the florets of different colors. CmbHLH2 generated two alternatively spliced transcripts, designated CmbHLH2Full and CmbHLH2Short. Compared with CmbHLH2Full, CmbHLH2Short encoded a truncated protein with only…

0106 biological sciences0301 basic medicinechrysanthemumMutantPlant Science01 natural sciencesanthocyaninSB1-1110alternative splicing03 medical and health scienceschemistry.chemical_compoundArabidopsisPigment accumulationOriginal Researchbasic helix–loop–helixbiologyChrysanthemum morifoliumAlternative splicingfood and beveragesPlant culturebiology.organism_classificationCell biology030104 developmental biologychemistryMBW complexAnthocyaninRNA splicingHeterologous expression010606 plant biology & botanyFrontiers in Plant Science
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Frequency-Dependent Selection in a Mammalian RNA Virus

1997

RNA viruses have been used as experimental systems to test evolutionary hypotheses such as Muller's ratchet (Chao 1990; Duarte et al. 1992, 1993; Clarke et al. 1993), the Red Queen hypothesis (Clarke et al. 1994), the nature of the adaptive topography (Elena, 1995; Elena et al. 1996), and the dynamics of adaptive evolution (Elena 1995; Novella et al. 1995). Two hypotheses which have received attention in virus studies are the competitive exclusion principle and frequency-dependent selection. The competitive exclusion principle (Hardin 1960) states that two populations or species competing for the same limiting resource cannot stably coexist because one competitor will displace the other. An…

0106 biological sciences0301 basic medicineeducation.field_of_studyExperimental evolutionmedia_common.quotation_subjectPopulationFrequency-dependent selectionViral quasispeciesBiology010603 evolutionary biology01 natural sciencesCompetition (biology)03 medical and health sciences030104 developmental biologyCompetitive exclusion principleEvolutionary biologyRed Queen hypothesisGeneticseducationGeneral Agricultural and Biological SciencesSelection (genetic algorithm)Ecology Evolution Behavior and Systematicsmedia_commonEvolution
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Selection for multicomponent mimicry: equal feature salience and variation in preferred traits

2016

When should multiple traits on Batesian mimics be selected to resemble corresponding traits on model species? Here, we explore two possibilities. First, features of equal salience to predators may be used to categorize prey, selecting for multicomponent mimicry. Second, if different predators use single yet different traits to categorize prey, multicomponent mimicry may still be selected. We studied how blue tits categorized rewarding and unrewarding artificial prey items that are differentiated by a combination of two color dimensions. Many birds used both color dimensions to make decisions, and overall, the population selected for multicomponent mimicry. However, a subset of birds used on…

0106 biological sciences0301 basic medicineeducation.field_of_studylearningindividual variationEcologyPopulationMultiple traitsovershadowingBiology010603 evolutionary biology01 natural sciencesBatesian mimicryPredation03 medical and health sciences030104 developmental biologyCategorizationEvolutionary biologySalience (neuroscience)Mimicryta1181Animal Science and Zoologycomplex signaleducationEcology Evolution Behavior and SystematicsBehavioral Ecology
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Evaluating responses to temperature during pre-metamorphosis and carry-over effects at post-metamorphosis in the wood tiger moth (Arctia plantaginis)

2019

Insect metamorphosis is one of the most recognized processes delimiting transitions between phenotypes. It has been traditionally postulated as an adaptive process decoupling traits between life stages, allowing evolutionary independence of pre- and post-metamorphic phenotypes. However, the degree of autonomy between these life stages varies depending on the species and has not been studied in detail over multiple traits simultaneously. Here, we reared full-sib larvae of the warningly coloured wood tiger moth ( Arctia plantaginis ) in different temperatures and examined their responses for phenotypic (melanization change, number of moults), gene expression (RNA-seq and qPCR of candidate ge…

0106 biological sciences0301 basic medicinelife-stage autonomymelanizationMothsWARNING SIGNALTrade-off01 natural sciencestäpläsiilikäsGENE-EXPRESSIONmedia_commonPOLYMORPHIC MOTHLarvamuodonvaihdosCOMPLEX LIFE-CYCLES70Metamorphosis BiologicalTemperaturewood tiger mothArticlesPhenotypeREAD ALIGNMENTPupacarry-over effectsTRADE-OFFLarva1181 Ecology evolutionary biologylämpötilaGeneral Agricultural and Biological SciencesResearch Article1001media_common.quotation_subjectZoologyLARVAL COLORBiology010603 evolutionary biologyGeneral Biochemistry Genetics and Molecular Biology03 medical and health sciencesAnimalsMetamorphosisADAPTIVE SIGNIFICANCElife stage autonomyWingTigerEVOLUTION030104 developmental biologyCOLOR PATTERNBasal metabolic ratehyönteisettranscriptomePhilosophical Transactions of the Royal Society B: Biological Sciences
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