Search results for "PLFA"

Local response of bacterial densities and enzyme activities to elevated atmospheric CO2 and different N supply in the rhizosphere of Phaseolus vulgar…

2008

Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699; Altered flux of labile C from plant roots into soil is thought to influence growth and maintenance of microbial communities under elevated atmospheric CO2 concentrations. We studied the abundance and function of the soil microbial community at two levels of spatial resolution to assess the response of microorganisms in the rhizosphere of the whole root system and of apical root zones of Phaseolus vulgaris L. to elevated CO2 and high or low N supply. At the coarser resolution, microb…

Determinazione degli acidi grassi dei fosfolipidi (PLFA) nel suolo

2010

Structural diversity and enzyme activity of volcanic soils at different stages of development and response to experimental disturbance

2008

We investigated the phospholipid fatty acid (PLFA) diversity and enzyme activities in soils from the volcano, Mt. Etna (Sicily). The soils were at sites which have been developing for different periods of time and have formed in volcanic lava of differing ages that have been supplemented with volcanic ejecta from subsequent eruptions. However, the plant communities indicated a marked successional difference between the sites and we have used this as a proxy for developmental stage. We have compared the structural and functional properties of the microbial communities in soils from the two sites and tested experimentally the hypothesis that the more diverse community was more resistant and r…

Do shifts in life strategies explain microbial community responses to increasing nitrogen in tundra soil?

2016

Subarctic tundra soils store large quantities of the global organic carbon (C) pool as the decomposition of plant litter and soil organic matter is limited by low temperatures and limiting nutrients. Mechanisms that drive organic matter decomposition are still poorly understood due to our limited knowledge of microbial communities and their responses to changing conditions. In subarctic tundra large grazers, in particular reindeer, exert a strong effect on vegetation and nutrient availability causing drastic nutrient pulses in the soils located along the migratory routes. Here we studied the effect of increased nitrogen (N) availability on microbial community structure and activities by lab…

The drivers of soil microbial communities structure on forest stands along the altitudinal gradient in western carpathians

2020

Abstract This study examines the effect of the soil physicochemical properties and the vegetation diversity and composition on the soil microorganism community structure on the temperate forest stands along the altitudinal gradient. The studied stands were located at three altitudes (600, 900, and 1200 m a.s.l.) on five separate mountain slopes (treated as independent replicates) in the Western Carpathians Mountains (Poland). The soil physicochemical characteristics varied widely between the stands on these same altitude, and only the organic matter content, water-holding capacity and dissolved organic nitrogen differed significantly between the different altitudes, exhibited highest values…

The impact of long-term water level draw-down on microbial biomass : A comparative study from two peatland sites with different nutrient status

2017

We examined the effects of long-term (51 years) drainage on peat microbial communities using phospholipid fatty acid (PLFA) analysis. We analysed the peat profiles of natural and adjacent drained fen and bog sites. Viable microbes (i.e. microbial PLFA) were present in relatively large amounts even in the deepest peat layers of both peatland sites, a finding that warrants further investigation. Microbial biomass was generally higher in the fen than in the bog. Microbial community structure (indexed from PLFA) differed between the fen and bog sites and among depths. Although we did not exclude other factors, the effect of drainage on the total microbial biomass and community structure was not…

Risposta dei bioindicatori della qualità del suolo alla diversa fertilizzazione di un aranceto nell’ Italia Meridionale

2013

Lo scopo della ricerca è stato quello di valutare i cambiamenti indotti dalle diverse tipologie di fertilizzazione sui parametri chimici e biologici di un suolo. Il suolo dell’agro di Lentini (SR, Sicilia), coltivato ad arancio da oltre 50 anni, a partire dal 1995 secondo il metodo biologico , omogeneo per portinnesto e cultivar, è stato addizionato con 4 diversi tipi di fertilizzanti (pollina, minerale, compost da pastazzo e letame). Quattro profili di suolo, ciascuno per tipo di fertilizzazione, sono stati campionati e studiati secondo la sequenza degli orizzonti genetici. In totale 18 campioni orizzonte-specifici di suolo sono stati prelevati e sottoposti ad analisi per valutare il conte…

Intrinsic bioremediation potential of a chronically polluted marine coastal area.

2015

A microbiological survey of the Priolo Bay (eastern coast of Sicily, Ionian Sea), a chronically polluted marine coastal area, was carried out in order to discern its intrinsic bioremediation potential. Microbiological analysis, 16S rDNA-based DGGE fingerprinting and PLFAs analysis were performed on seawater and sediment samples from six stations on two transects. Higher diversity and variability among stations was detected by DGGE in sediment than in water samples although seawater revealed higher diversity of culturable hydrocarbon-degrading bacteria. The most polluted sediment hosted higher total bacterial diversity and higher abundance and diversity of culturable HC degraders. Alkane- an…

Impact of Ag and Co engineered nanoparticles on soil microbial community structure in a soil perturbed by Lumbricus rubellus

2014

Knowledge on the impact of engineered nanomaterials (ENMs) on both human and environment health is scarce. Several studies sustain that soil is the environmental compartment designed to be the major recipient of engineered nanoparticles (NPs). With the aim of investigating the impact of commercially relevant NPs on soil functioning, we compared the effect of Ag and Co NPs, as well as cobalt and silver ions, on soil microbial community in the presence of Lumbricus rubellus. Earthworms specimens were placed in a rich‐C soil and fed with horse manure spiked with Ag‐NPs, Co‐NPs, Ag+ and Co2+ for a total amount of 10 mg of single pollutant kg‐1 soil. At the end of acute exposure (4 weeks) to pol…

Response of microbial biomass and carbon dynamics to changing hydrological conditions in old peat deposits

2018

Peatlands play key roles in the global ecosystem carbon (C) balance, as important C-stores. Their efficiency as long-term C-stores or atmospheric C sink is affected by the rate of microbial mineralization. Most studies on the climate change effects on the microbial communities and C-cycling in peatlands have focused on the surface layers. This thesis compared microbial biomass, community composition and internal C cycling processes in drained vs. natural peat profiles with focus on deep old peat. Using the phospholipid fatty acid (PLFA) and rRNA gene analyses, the study particularly looked at the impact of long-term drainage and the short-term effects of temperature and water content on the…