Search results for "PLL"
showing 10 items of 62 documents
POROUS SCAFFOLDS BASED ON PLLA/FUNCTIONALISED POLYMERS BLENDS PRODUCED BY THERMALLY INDUCED PHASE SEPARATION
2014
GENERATION OF PREVASCULARIZED PLLA BIODEGRADABLE SCAFFOLDS BY DIP DRAWING AND DIFFUSION INDUCED PHASE SEPARATION (DIPS).
2009
A critical obstacle in tissue engineering is to develop a massive structure of living cells upon transfer from the in vitro culture conditions into the host in vivo. A vascular network is required to supply essential nutrients, including oxygen, remove metabolic waste products and provide a biochemical communication “highway”. For these reasons to build an implantable structure in which vessel formation (angiogenesis) take place is mandatory. PLLA scaffolds usable in vascular tissue engineering were generated by dip-coating via Diffusion Induced Phase Separation (DIPS) technique. The scaffolds, with a vessel-like shape, were obtained by performing a DIPS process around a nylon fibre whose d…
Bio-affinity of MC3T3-E1 osteoblastic cells with polymeric scaffold in Poly L-Lactid Acid (PLLA) for bone substitution.
2009
Damage to an organ or tissue remains a problem despite advances in medical technology. Available treatments include organs transplantation, surgical reconstruction such as mechanical devices. However, all these methodologies have several contraindications. In recent years, tissue engineering methods is considered as means to replace diseased or damaged organs. For these kind of application, the choice of scaffolding material is crucial to the success of the technique. In the function of the application, the synthetic scaffolds should meet several criteria, including: good biocompatibility, sufficient mechanical properties, and adequate biodegradability. The aim of our study was to evaluate …
A COMPOSITE PLLA SCAFFOLD FOR REGENERATION OF COMPLEX TISSUES
2010
A composite biodegradable scaffold incorporating an integrated network of synthetic blood vessels was designed and prepared, in line with the requirements of a scaffold effectively supporting the regeneration of highly vascularized tissues. In other words, this composite scaffold should allow the regeneration of complex injured tissue (e.g. dermis) and, at the same time, favour the development of a vascular network on its inner, i.e. a 3D polymeric scaffolds embedding synthetic blood vessel-like structures for nutrient supply and metabolite removal. PLLA assures a high degree of biocompatibility and a low level of inflammation response upon implantation, while the embedded tubular vessel-li…
Tubular composite scaffolds produced via Diffusion Induced Phase Separation (DIPS) as a shaping strategy for anterior cruciate ligaments reconstructi…
Injuries of tendons and ligaments are common, especially among the young population. Anterior cruciate ligament (ACL) injuries do not heal due to its limited vascularization and hence, surgical intervention is usually required. The ideal scaffold for ligament tissue engineering (TE) should be biocompatible and possess mechanical and functional characteristics comparable to the native ACL. The Diffusion Induced Phase Separation (DIPS) technique allows the preparation of homogenous porous tubular scaffold with micro-pores using a rather simple procedure. Composites based on biodegradable polymers and bioglass have attracted much attention in tissue reconstruction and repair because of their b…
Biological characterization of Poly-L-lactic acid (PLLA)/Hydroxyapatite (HA)/Bioglass (BG) composite scaffolds made by Thermally Induced Phase Separa…
In the last few years, Tissue Engineering has focused on the favourable effects that composite scaffolds have on cell adhesion, growth and differentiation. In fact, composite scaffolds, usually composed of a synthetic polymer supplemented with naturally occurring components, display superior mechanical properties and bioconductivity than scaffolds consisting of a single component. Hydroxyapatite (HA) is the major inorganic component of bones. Bioglass (BG) is known to exert stimulatory effects on cells by ion release and hence, could be also advantageous for Bone Tissue Engineering. Poly-L-lactic acid (PLLA) is a versatile synthetic polymer combinable with HA and BG. The aim of this work wa…
Migration of brain capillary endothelial cells inside poly (lactic acid) 3D scaffolds
2017
The brain capillary endothelial cells (BCECs) form the blood brain barrier (BBB) under the effects of the brain microenvironment. BCECs are sealed together by tight junctions (TJs) that are responsible for the barrier phenotype. In these junctions, molecules such as JAM (junctional adhesion molecules), occludin and claudins are present. Threedimensional scaffolds are used to grow cells in order to obtain in vitro engineered tissues. On the base of these considerations, the aim of this work was to understand whether the endothelial cells were able to grow and survive on a new three-dimensional structure. If yes, indeed, this system could be further enriched and used to set a three-dimensiona…
Tubular scaffold for vascular tissue engineering application
2010
A critical obstacle in tissue engineering is the inability to maintain large masses of living cells upon transfer from the in vitro culture conditions into the host in vivo. Capillaries, and the vascular system, are required to supply essential nutrients, including oxygen, remove waste products and provide a biochemical communication “highway”. Another goal in this research field is the possibility to tune the biodegradability of the scaffold. After implantation, the scaffold has to be gradually replaced by cells and extra cellular matrix and it is crucial that this replacement takes place with an appropriate dynamics. A premature degradation, in fact, could lead to a collapse of the struct…
PLLA scaffolds based on thermally induced phase separation: morphology, cell seeding and proliferation
2009
Design of perfusion bioreactors and PLLA-based scaffolds for in vitro tissue engineering
2022
L'ingegneria tissutale rappresenta un nuovo approccio che integra cellule e matrici ingegnerizzate per la formazione di nuovi tessuti. In questa strategia, tre componenti essenziali costituiscono la cosiddetta triade della Tissue Engineering: segnali regolatori, cellule e scaffold tridimensionali (3D) biodegradabili e porosi. Tali elementi sono combinati per sviluppare un tessuto funzionale organizzato e 3D che simula la matrice extracellulare (ECM) del tessuto da rigenerare. Le funzioni specifiche dei tessuti nativi sono correlate agli ambienti complessi che, all'esterno del corpo, possono essere imitati usando degli strumenti chiamati bioreattori. Questi sistemi forniscono un ambiente in …