Search results for "PORES"
showing 10 items of 155 documents
Extensions of known geographic distribution of aphyllophoroid fungi (Basidiomycota) in Finland
2016
Article
The taxonomic position of Roesleria subterranea
2006
The genus Roesleria was introduced with the single species Roesleria hypogaea (current name: R. subterranea) by Thümen in 1877. The species was originally described from roots of grapevine and recognised as a facultative root-rotting parasite. The mazaediate ascoma with evanescent asci led to the assumption that Roesleria would be an ally of mazaediate lichens. In this study we calculate 28S, 18S as well ITS1-5.8S-ITS2 rDNA phylogenies. These data indicate that Roesleria is closely related to Hymenoscyphus (Helotiales). In contrast to other members of the Helotiales, the ascospores of Roesleria are passively released, and a distinct apical apparatus cannot be observed by LM. Electron optic …
Distributions of the growth rate of the germ tubes and germination time of Penicillium chrysogenum conidia depend on water activity
2008
The effects of water activities for sporulation (a(wsp)) and germination (a(wge)) on the distributions of the growth rate of the germ tubes (mu) and the germination time (t(G)) of Penicillium chrysogenum conidia were determined by monitoring the length of the same germ tubes throughout the experiments automatically. No relationship between the individual t(G)'s and mu's could be established. Irrespective of the water activity for germination, mu was greater and t(G) was less for conidia produced at 0.95a(wsp) than that at 0.99a(wsp). At 0.99 a(wge) the mean and the standard deviation of t(G) were smaller than those obtained at 0.95a(wge). At 0.99a(wge), normal distributions for mu and t(G) …
Lag time for germination of Penicillium chrysogenum conidia is induced by temperature shifts.
2013
In the environment, fungal conidia are subject to transient conditions. In particular, temperature is varying according to day/night periods. All predictive models for germination assume that fungal spores can adapt instantaneously to changes of temperature. The only study that supports this assumption (Gougouli and Koutsoumanis, 2012, Modelling germination of fungal spores at constant and fluctuating temperature conditions. International Journal of Food Microbiology, 152: 153-161) was carried out on Penicillium expansum and Aspergillus niger conidia that, in most cases, already produced germ tubes. In contrast, the present study focuses on temperature shifts applied during the first stages…
Fabrication of Single Cylindrical Au-Coated Nanopores with Non-Homogeneous Fixed Charge Distribution Exhibiting High Current Rectifications
2014
We have designed and characterized a cylindrical nanopore that exhibits high electrochemical current rectification ratios at low and intermediate electrolyte concentrations. For this purpose, the track-etched single cylindrical nanopore in polymer membrane is coated with a gold (Au) layer via electroless plating technique. Then, a non-homogeneous fixed charge distribution inside the Au-coated nanopore is obtained by incorporating thiol-terminated uncharged poly(N-isopropylacrylamide) (PNIPAM) chains in series to poly(4-vinyl pyridine) (PVP) chains, which are positively charged at acidic pH values. The functionalization reaction is checked by measuring the current–voltage (I–V) curves prior …
Metal ion affinity-based biomolecular recognition and conjugation inside synthetic polymer nanopores modified with iron-terpyridine complexes.
2011
Here we demonstrate a novel biosensing platform for the detection of lactoferrin (LFN) via metal-organic frameworks, in which the metal ions have accessible free coordination sites for binding, inside the single conical nanopores fabricated in polymeric membrane. First, monolayer of amine-terminated terpyridine (metal-chelating ligand) is covalently immobilized on the inner walls of the nanopore via carbodiimide coupling chemistry. Second, iron-terpyridine (iron-terPy) complexes are obtained by treating the terpyridine modified-nanopores with ferrous sulfate solution. The immobilized iron-terPy complexes can be used as recognition elements to fabricate biosensing nanodevice. The working pri…
Computational methods and theory for ion channel research
2022
Ion channels are fundamental biological devices that act as gates in order to ensure selective ion transport across cellular membranes; their operation constitutes the molecular mechanism through which basic biological functions, such as nerve signal transmission and muscle contraction, are carried out. Here, we review recent results in the field of computational research on ion channels, covering theoretical advances, state-of-the-art simulation approaches, and frontline modeling techniques. We also report on few selected applications of continuum and atomistic methods to characterize the mechanisms of permeation, selectivity, and gating in biological and model channels.
Generation of homozygosity and genome fixation in pea (Pisum sativum L.)
2013
Pea cultivars are nearly homozygous and thus homogeneous when they are released. The traditional method of selfing is slow and inefficient, taking up to ten generations of inbreeding following a cross to achieve a high level of homozygosity. Current single-seed-descent (SSD) methodologies enable a maximum of three generations per year to be developed in pea. Doubled haploidy and an in vitro based modified SSD technology have been utilised in many important crops for the rapid achievement of homozygosity, and thus acceleration of the breeding process. In pea, due to the lack of robust protocols, none of these technologies is routinely used in a breeding program. The aim of this study was to …
Assessment of antifungal activity of extracts from nettle (Urtica dioica L.) against Alternaria solani = Ocena aktywności przeciwgrzybowej ekstraktów…
2015
(Urtica dioica L.) w stężeniach 2,5, 5,0, 10,0, 20,0, 40,0% wobec fitopatogennego szczepu Alternaria solani. Aktywność przeciwgrzybową ekstraktów z pokrzywy określono w oparciu o indeks tempa wzrostu grzybni oraz indeks kiełkowania zarodników. W badaniach określono również wpływ metod sterylizacji testowanych wyciągów na ich aktywność. Na podstawie uzyskanych wyników wykazano, iż ekstrakty sterylizowane parą wodną pod ciśnieniem nie wykazują aktywności przeciwgrzybowej. W przypadku ekstraktu z korzenia, który charakteryzował się większą aktywnością inhibicyjną, uzyskano zaledwie 7% redukcję tempa wzrostu grzybni. Natomiast ekstrakt z korzenia poddany sterylizacji przez sączenie ograniczał o…
Polypore communities in broadleaved boreal forests
2012
The cover and extent of boreal broadleaved forests have been decreasing due to modern forest management practices and fire suppression. As decomposers of woody material, polypores are ecologically important ecosystem engineers. The ecology and conservation biology of polypores have been studied intensively in boreal coniferous forests. However, only a few studies have focused on the species living on broadleaved trees. To increase knowledge on this species group we conducted polypore surveys in 27 broadleaved forests and 303 forest compartments (539 ha) on the southern boreal zone in Finland and measured dead wood and forest characteristics. We detected altogether 98 polypore species, of wh…