Search results for "PRB"
showing 10 items of 22 documents
Nuclear and cytoplasmic interaction of pRb2/p130 and ER-β in MCF-7 breast cancer cells
2006
Estrogens exhibit important biological functions and influence several pathological processes of hormone-dependent diseases. The biological actions of estrogens require their interaction with two estrogen receptors (ER-alpha and ER-beta), which are ligand-dependent transcription factors. ER-alpha and ER-beta exhibit distinct tissue expression patterns as well as show different patterns of gene regulation. In addition, it has been suggested that ER-beta works as a counter partner of ER-alpha through inhibition of the transactivating functions of ER-alpha. For instance, ER-beta seems to play a different role in breast tumorigenesis than ER-alpha, as ER-beta decreased expression in breast canc…
Missing Evidences in Cancer Genetics: The Retinoblastoma Paradigm
2008
BACKGROUND: Retinoblastoma (Rb) is the most common primary malignant intraocular tumour in childhood. The "two hit" theory, formulated by Knudson in 1971 to explain the variegated clinical expression of the disease, led to the discovery of the so called tumour suppressor genes and the identification of the Rb1 as the prototype of such genes. Mutations of the Rb1 gene are now commonly believed to be the "cause" retinoblastoma, although epidemiological, clinical, and biological evidences argue against it. MATERIAL/METHODS: The Authors have performed a systematic review of available data concerning clinical and diagnostic aspects of retinoblastoma, including molecular genetics. Meta analysis o…
RB1 in cancer: Different mechanisms of RB1 inactivation and alterations of pRb pathway in tumorigenesis.
2013
Loss of RB1 gene is considered either a causal or an accelerating event in retinoblastoma. A variety of mechanisms inactivates RB1 gene, including intragenic mutations, loss of expression by methylation and chromosomal deletions, with effects which are species-and cell type-specific. RB1 deletion can even lead to aneuploidy thus greatly increasing cancer risk. The RB1gene is part of a larger gene family that includes RBL1 and RBL2, each of the three encoding structurally related proteins indicated as pRb, p107, and p130, respectively. The great interest in these genes and proteins springs from their ability to slow down neoplastic growth. pRb can associate with various proteins by which it …
Transient and stable depletion of RB induce different expression of genes involved in epigenetic modifications.
2008
Uso del nocciolino di oliva come materiale di riempimento di biotrincee per la denitrificazione biologica in situ di acque di falda
2016
Tra le varie possibilità di intervento per la rimozione dei nitrati dalle acque di falda, notevole interesse suscita il trattamento in situ dei plume contaminati mediante la realizzazione di biotrincee denitrificanti scavate in situ e naturalmente attraversate dalle acque di falda. Tra i materiali di origine organica che sono stati testati per tali applicazioni, il nocciolino di olive è risultato uno dei più idonei alle potenziali applicazioni di pieno campo. Tale tipo di materiale possiede un elevato contenuto di carbonio organico totale, prossimo al 50%, e una percentuale di lignina prossima al 45% in peso. Il lavoro proposto riporta i risultati di una campagna sperimentale eseguita media…
La denitrificazione biologica delle acque di falda: studio di tre differenti substrati organici
2015
L’uso massiccio di fertilizzanti contenenti azoto è causa dei fenomeni di inquinamento diffuso delle acque di falda. In tale ottica, notevole interesse hanno recentemente riscosso i trattamenti biologici in situ, in grado di promuovere la denitrificazione delle acque di falda mediante la realizzazione di interventi che coniugano efficacia di rimozione e costi relativamente contenuti. Nella nota si riportano i risultati di una ricerca sul processo di denitrificazione eterotrofa, condotta utilizzando come fonte di carbonio tre materiali convenzionalmente considerati di scarto: corteccia di pino, sughero e sansa di olive. Lo studio è stato suddiviso in due fasi: la prima condotta in modalità b…
Sviluppo di giunzioni ibride composito-metallo
2014
Three-dimensional matching based resource provisioning for the design of low-latency heterogeneous IoT networks
2019
Internet-of-Things (IoT) is a networking architecture where promising, intelligent services are designed via leveraging information from multiple heterogeneous sources of data within the network. However, the availability of such information in a timely manner requires processing and communication of raw data collected from these sources. Therefore, the economic feasibility of IoT-enabled networks relies on the efficient allocation of both computational and communication resources within the network. Since fog computing and 5G cellular networks approach this problem independently, there is a need for joint resource-provisioning of both communication and computational resources in the networ…
RNAi mediated acute depletion of Retinoblastoma protein (pRb) promotes aneuploidy in human primary cells via micronuclei formation
2009
BACKGROUND: Changes in chromosome number or structure as well as supernumerary centrosomes and multipolar mitoses are commonly observed in human tumors. Thus, centrosome amplification and mitotic checkpoint dysfunctions are believed possible causes of chromosomal instability. The Retinoblastoma tumor suppressor (RB) participates in the regulation of synchrony between DNA synthesis and centrosome duplication and it is involved in transcription regulation of some mitotic genes. Primary human fibroblasts were transfected transiently with short interfering RNA (siRNA) specific for human pRb to investigate the effects of pRb acute loss on chromosomal stability. RESULTS: Acutely pRb-depleted fibr…
pRb suppresses camptothecin-induced apoptosis in human osteosarcoma Saos-2 cells by inhibiting c-Jun N-terminal kinase
2001
AbstractThis paper studies the cytotoxic effect induced by the topoisomerase I inhibitor camptothecin in human osteosarcoma Saos-2 cells, which lack p53 and contain a non-functional form of the product of the retinoblastoma gene, pRb. Cytotoxicity induced by camptothecin was dose- and time-dependent; the treatment with 100 nM camptothecin reduced cell viability by 50% at 32 h and by 75% at 72 h of exposure. The cytotoxic effect was caused by apoptosis, as ascertained by morphological evidence, acridine orange-ethidium bromide staining and flow cytometric analysis. Apoptosis was accompanied by both the activation of caspase-3 and the fragmentation of poly(ADP-ribose) polymerase. Treatment wi…