Search results for "PRECIPITATION"

showing 10 items of 826 documents

Estudio climático del exponente “n” de las curvas IDF: aplicación para la Península Ibérica

2009

El análisis de las precipitaciones máximas suele llevarse a cabo mediante curvas IDF (Intensidad-Duración-Frecuencia), que a su vez pueden expresarse como curvas IMM (Intensidades Medias Máximas). En este trabajo, hemos desarrollado un índice “n”, definido a partir del exponente que se obtiene de ajustar las curvas climáticas IDF a las curvas IMM. Dicho índice proporciona información sobre el modo en que se alcanzan las precipitaciones máximas en una determinada zona clim´atica, atendiendo a la distribuci´on temporal relativa de las intensidades m´aximas. A partir del an´alisis clim´atico del ´ındice “n”, en la Pen´ınsula Ib´erica se pueden distinguir grandes zonas caracterizadas por m´axim…

Curvas IMMUNESCO::CIENCIAS DE LA TIERRA Y DEL ESPACIO::ClimatologíaRegularity indexCurvas IDF:CIENCIAS DE LA TIERRA Y DEL ESPACIO::Climatología [UNESCO]Intensidad precipitaciónIDF curvesPrecipitation intensityÍndice regularidadMAI curves
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Amyloid Precursor-like Protein 1 Influences Endocytosis and Proteolytic Processing of the Amyloid Precursor Protein

2005

Ectodomain shedding of the amyloid precursor protein (APP) is a key regulatory step in the generation of the Alzheimer disease amyloid beta peptide (Abeta). The molecular mechanisms underlying the control of APP shedding remain little understood but are in part dependent on the low density lipoprotein receptor-related protein (LRP), which is involved in APP endocytosis. Here, we show that the APP homolog APLP1 (amyloid precursor-like protein 1) influences APP shedding. In human embryonic kidney 293 cells expression of APLP1 strongly activated APP shedding by alpha-secretase and slightly reduced beta-secretase cleavage. As revealed by domain deletion analysis, the increase in APP shedding re…

CytoplasmTime FactorsRecombinant Fusion ProteinsAmino Acid MotifsBlotting WesternGenetic VectorsEndocytic cycleCHO CellsTransfectionEndocytosisBiochemistryCell LineAmyloid beta-Protein PrecursorGenes ReporterCricetinaeChlorocebus aethiopsEndopeptidasesmental disordersAmyloid precursor proteinAnimalsAspartic Acid EndopeptidasesHumansImmunoprecipitationAPLP1Molecular BiologyModels GeneticbiologyChemistryHEK 293 cellsP3 peptideCell BiologyEndocytosisProtein Structure TertiaryMicroscopy FluorescenceBiochemistryAlpha secretaseEctodomainCOS Cellsbiology.proteinAmyloid Precursor Protein SecretasesPeptidesGene DeletionPlasmidsJournal of Biological Chemistry
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Nuclear Translocation of Papillomavirus Minor Capsid Protein L2 Requires Hsc70

2004

ABSTRACT Minor capsid protein L2 of papillomaviruses plays an essential role in virus assembly by recruiting viral components to PML bodies, the proposed sites of virus morphogenesis. We demonstrate here that the function of L2 in virus assembly requires the chaperone Hsc70. Hsc70 was found dispersed in naturally infected keratinocytes and cultured cells. A dramatic relocation of Hsc70 from the cytoplasm to PML bodies was induced in these cells by L2 expression. Hsc70-L2 complex formation was confirmed by coimmunoprecipitation. The complex was modulated by the cochaperones Hip and Bag-1, which stabilize and destabilize Hsc70-substrate complexes, respectively. Cytoplasmic depletion of Hsc70 …

Cytoplasmanimal structuresImmunoprecipitationvirusesImmunologyActive Transport Cell Nucleusmacromolecular substancesBiologyMicrobiologyVirusGreen fluorescent proteinCell Line TumorVirologyAnimalsHSP70 Heat-Shock ProteinsCOS cellsHSC70 Heat-Shock ProteinsVirionOncogene Proteins ViralMolecular biologyVirus-Cell InteractionsTransport proteinCell biologyProtein TransportCapsidCytoplasmInsect ScienceChaperone (protein)COS Cellsembryonic structuresbiology.proteinCapsid ProteinsJournal of Virology
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Cyclin-dependent kinase inhibitor p21 controls adult neural stem cell expansion by regulating Sox2 gene expression.

2012

Summary In the adult brain, continual neurogenesis of olfactory neurons is sustained by the existence of neural stem cells (NSCs) in the subependymal niche. Elimination of the cyclin-dependent kinase inhibitor 1A (p21) leads to premature exhaustion of the subependymal NSC pool, suggesting a relationship between cell cycle control and long-term self-renewal, but the molecular mechanisms underlying NSC maintenance by p21 remain unexplored. Here we identify a function of p21 in the direct regulation of the expression of pluripotency factor Sox2, a key regulator of the specification and maintenance of neural progenitors. We observe that p21 directly binds a Sox2 enhancer and negatively regulate…

Cèl·lules mare neuralsCyclin-Dependent Kinase Inhibitor p21Chromatin ImmunoprecipitationImmunoblottingArticle03 medical and health sciencesMice0302 clinical medicineSOX2Neural Stem CellsCyclin-dependent kinaseNeurosphereSubependymal zoneGeneticsExpressió genèticaAnimalsProgenitor cellCells Cultured030304 developmental biology0303 health sciencesbiologyCell growthReverse Transcriptase Polymerase Chain ReactionSOXB1 Transcription FactorsNeurogenesisCell BiologyImmunohistochemistryNeural stem cellMice Mutant Strains3. Good healthAdult Stem Cellsnervous systemCancer researchbiology.proteinMolecular Medicinebiological phenomena cell phenomena and immunity030217 neurology & neurosurgeryProtein BindingCell stem cell
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Transcription of genes in the biosynthetic pathway for fumonisin mycotoxins is epigenetically and differentially regulated in the fungal maize pathog…

2012

ABSTRACT When the fungal pathogen Gibberella moniliformis (anamorph, Fusarium verticillioides ) colonizes maize and maize-based products, it produces class B fumonisin (FB) mycotoxins, which are a significant threat to human and animal health. FB biosynthetic enzymes and accessory proteins are encoded by a set of clustered and cotranscribed genes collectively named FUM, whose molecular regulation is beginning to be unraveled by researchers. FB accumulation correlates with the amount of transcripts from the key FUM genes, FUM1 , FUM21 , and FUM8 . In fungi in general, gene expression is often partially controlled at the chromatin level in secondary metabolism; when this is the case, the deac…

DISRUPTIONTranscription GeneticFUM21[SDV]Life Sciences [q-bio]DIVERSITYPROTEINFusarium verticillioidesmaizeSECONDARY METABOLISMgene clusterEpigenesis GeneticHistonesFUM8FusariumGene Expression Regulation FungalASPERGILLUSPromoter Regions Genetic2. Zero hungerGenetics0303 health sciencesHistone deacetylase inhibitorhistone acetylationAcetylationArticlesGeneral MedicineChromatinChromatinGENOMEHistoneMultigene Family[SDE]Environmental SciencesTrichostatin AEpigenetics; Fusarium verticillioides; fmonisin synthesismedicine.drugCONIDIATIONChromatin Immunoprecipitationmedicine.drug_classGenes FungalChIPBiologyGFPZea maysMicrobiologyFumonisinsChromatin remodeling03 medical and health sciencesmedicineEpigeneticsMolecular Biology030304 developmental biologyepigenetics030306 microbiologyCLUSTERFumonisins; epigenetics; Fusarium verticillioides; maize; histone acetylation; histone deacetylases; ChIP; Trichostatin A; FUM1; FUM21; FUM8; GFP; gene clusterMycotoxinsChromatin Assembly and DisassemblyFUM1Histone Deacetylase InhibitorsTrichostatin AAcetylationbiology.proteinChromatin immunoprecipitationhistone deacetylases
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Parvovirus B19 nonstructural protein-induced damage of cellular DNA and resultant apoptosis.

2010

Parvovirus B19 is a widespread virus with diverse clinical presentations. The viral nonstructural protein, NS1, binds to and cleaves the viral genome, and induces apoptosis when transfected into nonpermissive cells, such as hepatocytes. We hypothesized that the cytotoxicity of NS1 in such cells results from chromosomal DNA damage caused by the DNA-nicking and DNA-attaching activities of NS1. Upon testing this hypothesis, we found that NS1 covalently binds to cellular DNA and is modified by PARP, an enzyme involved in repairing single-stranded DNA nicks. We furthermore discovered that the DNA nick repair pathway initiated by poly(ADPribose)polymerase and the DNA repair pathways initiated by …

DNA RepairDNA damageViral nonstructural proteinDNA repairPoly ADP ribose polymerasevirusesBlotting WesternParvovirus B19Viral Nonstructural ProteinsCell Linechemistry.chemical_compoundsystemic lupus erythematosusParvovirus B19 HumanHumansImmunoprecipitationPolymerasebiologyfulminant liver failureDNA damage and repairapoptosisvirus diseasesGeneral MedicineTransfectionMolecular biologyProliferating cell nuclear antigenchemistrybiology.proteinDNAautoantibodyDNA DamageResearch PaperInternational journal of medical sciences
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Influence of molecular weight of DNA on the determination of anti-DNA antibodies in systemic lupus erythematosus (SLE) sera by radioimmunoassay

1975

Using a radioimmunoassay (RIA) based on the Farr technique with radioactively labeled 3-H-DNA for quantitative measurements of anti-DNA antibodies in sera of patients with systemic lupus erythematosus (SLE), the influence of molecular weight of DNA (ranging from 0.1 times 10-6 to 22.0 times 10-6 daltons) on binding and precipitation in this system has been investigated. Comparing our results with mathematical models it follows that one antibody molecule is fixed on the average to a statistical DNA segment of 2 times 10-6 to 4 times 10-6 daltons. Furthermore binding capacity of the DNA was found to be independent of the molecular weight, as demonstrated in a double label experiment using 14-…

DNA BacterialRadioimmunoassayBiologyModels BiologicalAntibodieschemistry.chemical_compoundGeneticsmedicineChemical PrecipitationLupus Erythematosus SystemicBinding siteAnti dnaLupus erythematosusMolecular massRadioimmunoassayDNAmedicine.diseaseMolecular biologyMolecular WeightAntibody moleculechemistryBiochemistrybiology.proteinBinding Sites AntibodyAntibodyDNANucleic Acids Research
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Overexpression and functional characterization of kinin receptors reveal subtype-specific phosphorylation.

1999

G protein-coupled receptors such as the receptors for bradykinin are present in low copy numbers in most natural cells. To overcome the problems associated with the analysis of these receptors at the protein level, we used highly efficient expression systems such as the baculovirus/insect cell system. However, the structural and functional statuses of recombinant receptors have often remained elusive. We have expressed the two types of human kinin receptors, B1 and B2, in Sf9 cells. Both receptors are found on the surface of infected cells where they display the same pharmacological profiles as their cognate receptors of native cells. The functional analysis of kinin receptors coupled to th…

DNA ComplementaryReceptor Bradykinin B2ImmunoprecipitationSf9SpodopteraBradykininReceptor Bradykinin B1TransfectionBiochemistryAnimalsHumansBinding siteCloning MolecularPhosphorylationReceptorMicroscopy ConfocalKinaseChemistryReceptors BradykininCell MembraneKininMolecular biologyRecombinant ProteinsCell biologyKineticsPhosphorylationCalciumIntracellularBiochemistry
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Analysis of the linkages between rainfall and land surface conditions in the West African monsoon through CMAP, ERS-WSC, and NOAA-AVHRR data

2005

International audience; The European Remote Sensing Wind Scatterometer (ERS-WSC) backscattering coefficient, NOAA Advanced Very High Resolution Radiometer (NOAA-AVHRR) Normalized Difference Vegetation Index (NDVI), and Climate Prediction Center Merged Analysis Precipitation ( CMAP) precipitation data sets are studied over the period August 1991 to December 2000 to document ( 1) the interannual and intra-annual evolutions of vegetation photosynthetic activity and soil-vegetation water content over West Africa and ( 2) their two-way links with precipitation. Over the Sahel, at interannual timescales the strongest relationships between vegetation, soil moisture, and precipitation are observed …

DYNAMICSAtmospheric Science010504 meteorology & atmospheric sciencesAdvanced very-high-resolution radiometerDIFFERENCE VEGETATION INDEX0211 other engineering and technologiesSoil ScienceTIME-SERIES02 engineering and technologyWIND SCATTEROMETER DATAAquatic ScienceOceanographyMonsoonSOIL-MOISTURE01 natural sciencesNormalized Difference Vegetation Index[SDV.EE.ECO]Life Sciences [q-bio]/Ecology environment/EcosystemsGeochemistry and PetrologyCIRCULATIONSEarth and Planetary Sciences (miscellaneous)[ SDU.ENVI ] Sciences of the Universe [physics]/Continental interfaces environmentPrecipitation[SDU.ENVI]Sciences of the Universe [physics]/Continental interfaces environmentWater contentTEMPERATURE021101 geological & geomatics engineering0105 earth and related environmental sciencesEarth-Surface ProcessesWater Science and TechnologyEcologyMoisturePaleontologyForestry15. Life on landScatterometerVARIABILITYGeophysics13. Climate actionSpace and Planetary ScienceClimatologyPRECIPITATIONSoil waterEnvironmental scienceSAHEL RAINFALL
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Towards a new generation of high-resolution meteorological input data for small-scale hydrologic modeling

2011

Summary Current and future challenges of hydrologic sciences are to accurately predict and assess climate-driven impacts on water resources for the relevant scales of planning. However, process-based small-scale hydrologic modeling is data demanding and large uncertainties exist in data-sparse areas. The aim of our study was to test the applicability of the COSMO-DE analysis data (COSMO-DE-A) for hydrologic modeling. COSMO-DE-A data are a new meteorological data set with high temporal and spatial resolution that originates from the German Weather Service data assimilation system using the COSMO-DE weather prediction model. We collected field parameters in a small (10 km 2 ) mountainous catc…

Data setData assimilationMeteorologyHydrological modellingLatent heatReference data (financial markets)Data analysisEnvironmental scienceHydrographPrecipitationWater Science and TechnologyJournal of Hydrology
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