Search results for "PRECIPITATION"
showing 10 items of 826 documents
Hepatitis B Virus Large Envelope Protein Interacts with γ2-Adaptin, a Clathrin Adaptor-Related Protein
2001
ABSTRACT For the outcome of a hepatitis B virus (HBV) infection, the viral L envelope protein with its pre-S domain performs pivotal functions by mediating attachment of HBV to liver cells, envelopment of viral capsids, release of (sub)viral particles, regulation of supercoiled DNA amplification, and transcriptional transactivation. To assess its multiple functions and host-protein assistance involved, we initiated a two-hybrid screen using the L-specific pre-S1 domain as bait. With this approach, we have identified γ2-adaptin, a putative member of the clathrin adaptor proteins responsible for protein sorting and trafficking, as a specific binding partner of L protein. Evidence for a physic…
Evaluation of activated sludge model no.2 at high phosphorus concentrations
2001
This paper presents laboratory scale experimentation carried out to study enhanced biological phosphorus removal at high phosphorus concentrations in a sequencing batch reactor. Four series of data obtained in a sequencing batch reactor are examined in light of the Activated Sludge Model No. 2. This model was calibrated using data from the first and second series working at low phosphorus concentrations. The Activated Sludge Model No. 2 successfully characterised the enhanced biological phosphorus removal performance of the sequencing batch reactor at low phosphorus concentrations. The calibrated model was then used to adjust experimental results of the other series working at high phosphor…
Possible Ca 2+ -dependent mechanism of apical outer hair cell modulation within the cochlea of the guinea pig
1998
Calcium ions were precipitated with potassium antimonate after injection of the inorganic calcium channel blocker MnCl2 or the inorganic potassium channel blockers BaCl2 or CsCl into the perilymph of the scala vestibuli of the guinea pig. The spatial distribution of the formed histochemical reaction products within the organ of Corti was studied by energy-filtering transmission-electron microscopy. Compared with untreated control ears, the number of the formed precipitates drastically increased at the extracellular side of the lamina reticularis after application of the various inorganic channel blockers. The apical side of the outer hair cells and the intervening Deiter cells were covered …
Hsp70 localizes differently from chaperone Hsc70 in mouse mesoangioblasts under physiological growth conditions
2008
Mouse A6 mesoangioblasts express Hsp70 even in the absence of cellular stress. Its expression and its intracellular localization were investigated under normal growth conditions and under hyperthermic stress. Immunofluorescence assays indicated that without any stress a fraction of Hsp70 co-localized with actin microfilaments, in the cell cortex and in the contractile ring of dividing cells, while the Hsc70 chaperone did not. Hsp70 immunoprecipitation assays confirmed that a portion of Hsp70 binds actin. Immunoblot assays showed that both proteins were present in the nucleus. After heat treatment Hsp70 and actin continued to co-localize in the leading edge of A6 cells but not on microfilame…
Modification of the major tegument protein pp65 of human cytomegalovirus inhibits virus growth and leads to the enhancement of a protein complex with…
2010
The tegument protein pp65 of human cytomegalovirus (HCMV) is abundant in lytically infected human foreskin fibroblasts (HFF), as well as in virions and subviral dense bodies (DB). Despite this, we showed previously that pp65 is dispensable for growth in HFF. In the process of refining a DB-based vaccine candidate, different HCMV mutants were generated, expressing a dominant HLA-A2-presented peptide of the IE1 protein fused to pp65. One of the mutant viruses (RV-VM1) surprisingly showed marked impairment in virus release from HFF. We hypothesized that analysis of the phenotypic alterations of RV-VM1 would provide insight into the functions of pp65, poorly defined thus far. RV-VM1 infection r…
Long-term changes of precipitation in Latvia
2010
This study investigated long-term variability and trends in Latvia's annual, seasonal, monthly and daily precipitation using data from 10 meteorological stations for the period 1925–2006 and from station Riga University for the period 1850–2006. The obtained results indicate that during the 20th century a significant increase in precipitation has occurred in the cold season while the warm period showed a decreasing tendency. The annual precipitation totals showed a slight decrease, at half of the studied stations, due to opposite tendencies in cold season and warm season. The long-term trend in the annual precipitation in Riga (from 1850) was positive with large interannual and interdecadal…
Flash floods in Mediterranean ephemeral streams in Valencia Region (Spain)
2016
Summary Ephemeral streams are typical Mediterranean fluvial systems with high risk of flash flooding, and few data are available about these systems in most locales. However, the SAIH network (Automatic Hydrological Information Systems) of the Jucar River Water Authority has been providing detailed information about ephemeral streams in Spain every 5 min since 1988. Using these data, we evaluated the processes of rainfall–runoff conversion and flood generation in five Mediterranean ephemeral streams ranging in size from 25 to 450 km 2 . To provide a general framework for hydrological analysis, the study included 142 flash flood events registered between 1989 and 2007. A more detailed analys…
Climatic changes in yield index and soil water deficit trends in China
2000
Long-term trends of the combined effects of evapotranspiration and precipitation effect surface hydrology and soil water and consequently natural and agricultural ecosystems. This paper analyses yield index and soil water deficit time series derived from water balance calculations for multiple cropping systems with FAO methodology. The analysis shows that yield index values have increased and soil water deficits have consequently decreased over much of China during 1954‐1993. The likely parameters contributing to this trend are precipitation changes north of 35 N and maximum evapotranspiration as well as available soil water trends south of this line. Increasing the assumed maximum soil wat…
Tropical Andean forest derives calcium and magnesium from Saharan dust
2008
[1] We quantified base metal deposition to Amazonian montane rain forest in Ecuador between May 1998 and April 2003 and assessed the response of the base metal budget of three forested microcatchments (8–13 ha). There was a strong interannual variation in deposition of Ca [4.4–29 kg ha−1 a−1], Mg [1.6–12], and K [9.8–30]). High deposition changed the Ca and Mg budgets of the catchments from loss to retention, suggesting that the additionally available Ca and Mg was used by the ecosystem. Increased base metal deposition was related to dust outbursts of the Sahara and an Amazonian precipitation pattern with trans-regional dry spells allowing for dust transport to the Andes. The increased base…
Climate change impacts on river runoff in Latvia
2011
In order to assess climate change impacts on river runoff patterns at the end of this cen- tury, the hydrological model METQ2007BDOPT was applied to 8 river basins and sub-basins in Latvia, which is a part of the southeast Baltic Sea basin. The climate data we used originate from the PRUDENCE project and were prepared in a separate study. Changes in hydro-climate were analysed using one control run (1961-1990) and 2 IPCC scenario runs (A2 and B2; 2071-2100). For the A2 sce- nario, both annual and seasonal analysis predicted the major significant changes in most cases. For both scenarios, an increase of the mean annual climate data (air temperature, precipitation and evapo - transpiration) i…