Search results for "PROPAGATION"
showing 10 items of 676 documents
Effect of weak magnetic fields on the in vitro propagation of Genista aetnensis (Raf. Ex Biv.) Dc.
2017
Over the years, many studies have emphasized the importance of the magnetic fields (MF), used as a safe alternative choice to improve agricultural production. The induction effect of different magnetic fields varies depending on the species, explants typology, intensity of magnetic field and period of exposure. The aim of the present study was to investigate the application of a continuous magnetic field induction, at different exposure times, as a production enhancement for in vitro culture of Genista aetnensis, an endemic shrub commonly named 'Mount Etna broom'. An in vitro protocol has been settled for the conservation of the species. Plantlets cultured onto a solified Murashige and Skoo…
In vitro propagation of the relict laperinne’s olive (Olea europaea L. subsp. laperrinei)
2017
Olea europaea L. subsp. laperrinei (Oleaceae) is an endemic taxon of the mountainous regions of central Sahara, consisting of currently fragmented and small relict populations. The tree can propagate vegetatively or by seed, but no recent natural regeneration was observed in the Algerian massive populations, some of which are considered threatened with extinction. Sterile triploid individuals were also identified in some populations showing increasing vigour. As a result of its long persistence and despite its rarity, the Laperrine's olive is an iconic component of Saharan mountain ecosystems. The aim of this study is to develop an efficient micropropagation protocol for both diploid and tr…
Arbuscular mycorrhizal fungi and micropropagation of high value crops
2002
Micropropagation has established its position as a way of propagating large numbers of uniform plants. For some plant species that are difficult to propagate by seeds or by conventional cuttings, this technique provides the only possible way of producing high quality plants. Micropropagation is widely used for propagation of high value crops like ornamentals, fruits, vegetables, plantation crops and spices (Vestberg and Estaun 1994). The micropropagation industry was growing fast in Europe up to 1992 (O’Riordain 1992) but after that the micropropagation industry seems to have stabilized its position although a slight increase in production of microplants was still recorded for the period 19…
Is in vitro micrografting a possible valid alternative to traditional micropropagation in Cactaceae? Pelecyphora aselliformis as a case study
2016
Several taxa of Cactaceae are endangered by overcollection for commercial purposes, and most of the family is included in the Convention on International Trade in Endangered Species of Fauna and Flora (CITES). Micropropagation may play a key role to keep the pressure off wild populations and contribute to ex situ conservation of endangered taxa. One of the limits of micropropagation is the species-specific requirement of plant regulators for each taxon and sometimes even for different genotypes. With the micrografting technique the rootstock directly provides the scion with the necessary hormonal requirements. In this paper we present data on in vitro grafting of Pelecyphora aselliformis Eh…
Evaluation of some growth regulator effects on encapsulated in vitro-derived microcuttings of three Italian Ficus carica L. genotypes.
2017
In this study, the encapsulation technology based on the calcium alginate coating was applied to some Ficus carica L. genotypes. Uninodal microcuttings (3-4 mm long), excised from in vitro proliferating shoots of three Italian cultivars (‘Bifera’, ‘Palazzo’ and ‘Catalanisca’), were employed. The influence of three different plant growth regulators (PGRs): 6-benzylaminopurine (BAP), meta-topolin (MT) and zeatine (ZEA), added to the artificial endosperm, were evaluated. Particularly, the viability, regrowth and conversion parameters of the synthetic seeds were registered after 60 days from the sowing on a hormone-free medium. Results showed that the cultivars ‘Catalanisca’ and ‘Palazzo’ showe…
Different bacteria can enhance the micropropagation response of Cotoneaster lacteus (Rosaceae)
1998
C. MONIER, E. BOSSIS, C. CHABANET AND R. SAMSON. 1998. Cotoneaster species are widely used ornamental shrubs, and research is under way to select genotypes which are more resistant to fire blight by somaclonal variation. During the establishment of micropropagation, one genotype, Cotoneaster lacteus, showed the development of endogenous bacterial contaminants. Two strains were isolated from in vitro explants: Bacillus pumilus and Alcaligenes faecalis. These two strains, and some Pseudomonas fluorescens and Ps. putida, promoted in vitro multiplication and rooting of explants. In Pseudomonas strains, rooting enhancing capacity appeared to be related to indole-3-acetic acid (IAA) production, a…
Grafting affects yield and phenolic profile of Solanum melongena L. landraces
2016
Abstract The influence of grafting on agronomical and qualitative characteristics of four Sicilian eggplant landraces was investigated. Grafted and ungrafted plants were compared in the open field in the northern coast of Sicily. Solanum torvum seedlings were used as rootstock. Regardless of genotypes tested, grafting significantly increased total fruit production, marketable production, and number of marketable fruits, but did not affect weight of marketable fruits and waste production. Landrace 2 (Sciacca), with black epidermal tissue and pyriform fruit shape, when grafted onto S. torvum not only gave a higher yield performance than ungrafted plants, but also showed a higher phenolic anti…
Microbial inoculation for improving the growth and health of micropropagated strawberry
2004
Multimicrobial inoculation has been proposed as a way of protecting plants against environmental stress and increasing the sustainability of plant production. To study these possibilities in a micropropagation system, microplants of strawberry, Fragaria × ananssa, were inoculated or left uninoculated with five microorganisms ( Glomus mosseae BEG29, Bacillus subtilis M3, Trichoderma harzianumDB11, Pseudomonas fluorescensC7r12 and Gliocladium catenulatumGliomix ® ), used either singly or in dual mixtures in the presence or absence of the strawberry diseases crown rot ( Phytophthora cactorum) and red stele (P. fragariae). Finnish light Sphagnum peat was used as the growth substrate in the expe…
Nuclear rDNA instability in in vitro-generated plants is amplified after sexual reproduction with conspecific wild individuals
2016
Using micropropagation through tissue culture has become the most used approach worldwide for mass production for the conservation of endangered species. However, the screening of somaclonal variations generated using in vitro culture is usually restricted to the first generation of micropropagated plants, when they have not yet been released in the field. Accordingly, the fate of genetically modified regenerants after sexual reproduction is usually not assessed and changes in the genetic structures of species are unknown. In this work, we assess the cytogenetic stability of two rDNA gene families in the offspring of experimental crosses between accessions generated after in vitro culture a…
An eco-physiological and biotechnological approach to conservation of the world-wide rare and endangered aquatic liverwort Riella helicophylla (Bory …
2016
AbstractThe rare aquatic liverwortRiella helicophylla(Bory et Mont.) Mont., inhabitant of temporary shallow ponds around the Mediterranean basin, is considered threatened throughout its distribution range. In addition, little is known of its biology and ecology or of its role in such an important ecosystem where environmental conditions vary yearly in unpredictable ways. In these variable habitats, due to the seasonal fluctuation of water levels, there is no guarantee of yearly spore input into the spore bank. Spore germination rate and the effects of different culture media in an axenic culture establishment, as well as propagation procedures ofR. helicophylla,were tested. New insights int…