Search results for "PTO"
showing 10 items of 28599 documents
Recombinant expression of the N-terminal domain of human T1R2 taste receptor: interaction with brazzein, a sweet-tasting protein
2014
Interaction of the n-terminal domain of human t1r2 taste receptor with brazzein, a sweet-tasting protein
2015
Brazzein is a small (6.5 kDa) sweet-tasting protein originating from the fruit of Pentadiplandra brazzeana, a plant found in West Africa. Brazzein like all classes of sweet compounds is perceived through the activation of the T1R2/T1R3 heterodimeric sweet-taste receptor. T1R2 and T1R3 subunits are members of the small family of class C G-protein coupled receptors (GPCRs). Class C GPCRs possess a large N-terminal domain (NTD) linked to seven transmembrane domain by a cysteine rich domain (CRD). The NTD of T1R2 (T1R2-NTD) has been shown to contain the primary binding site for most of the sweet ligands. However, brazzein has been shown to require CRD of human T1R3 for receptor activation [1]. …
Expression hétérologue, repliement in vitro et caractérisation biophysique du domaine N-terminal de la sous-unité T1R3 du récepteur humain au goût su…
2010
The sweet taste receptor is a heterodimer composed of two subunits called T1R2 and T1R3. Each subunit belongs to the class C of G protein-coupled receptors and is constituted by a large extracellular N-terminal domain (NTD) linked to the transmembrane domain by a cysteine-rich region. It has been shown that T1R2 and T1R3 NTDs are both able to bind natural sugars and sucralose with distinct affinities and undergo ligand-dependent conformational change (Nie et al., Curr Biol, 2005). However, the binding properties of T1R3 NTD and the relative contribution of the two subunits to the heterodimeric receptor function remained largely unknown. To characterize the binding properties of each subunit…
Role of odorant-binding proteins in Drosophila melanogaster chemosensory perception
2019
Chemoperception is used by animals to detect nutritive food and avoid toxic compounds. It also allows animals to identify suitable ecological niche and mating partners. Like many other insects, Drosophila melanogaster possesses a very sensitive chemosensory ability and can detect and discriminate a wide panel of semiochemicals. Chemosensory detection is mostly mediated by olfactory and gustatory systems involving several multigene chemoreceptor families. Volatile and non-volatile chemical compounds entering the sensory organ (sensillum) must be solubilized before being transported through the hydrophilic sensillum lymph bathing the dendrites of chemosensory neurons. These perireceptor event…
Chemicals and chemoreceptors: ecologically relevant signals driving behavior in Drosophila
2015
Insects encounter a vast repertoire of chemicals in their natural environment, which can signal positive stimuli like the presence of a food source, a potential mate, or a suitable oviposition site as well as negative stimuli such as competitors, predators, or toxic substances reflecting danger. The presence of specialized chemoreceptors like taste and olfactory receptors allows animals to detect chemicals at short and long distances and accordingly, trigger proper behaviors toward these stimuli. Since the first description of olfactory and taste receptors in Drosophila melanogaster 15 years ago, our knowledge on the identity, properties, and function of specific chemoreceptors has increase…
Taste perception: from molecule to eating behaviour
2020
The sense of taste has a role in human nutrition, it help to orient our food choices and avoid the ingestion of toxi compounds. Advances in molecular biology, cell biology an, biochemistry were used to identify and characterize the main taste receptors. The identification of these receptors allow a better understanding of the physiological processes tha govern taste perception. The presence of genetic polymorphisr in these receptors explains interindividual differences in tast perception.
Branching Ratios and Spectral Functions of $\tau$ Decays: final ALEPH measurements and physics implications
2005
The full LEP-1 data set collected with the ALEPH detector at the $Z$ pole during 1991-1995 is analysed in order to measure the $\tau$ decay branching fractions. Extensive systematic studies are performed, in order to match the large statistics of the data sample corresponding to over 300 000 measured and identified $\tau$ decays. Branching fractions are obtained for the two leptonic channels and eleven hadronic channels defined by their respective numbers of charged particles and $\pi^0$'s. Using previously published ALEPH results on final states with charged and neutral kaons, corrections are applied to the hadronic channels to derive branching ratios for exclusive final states without kao…
Identificación de TCRs reactivos contra neoantígenos tumorales en muestras de pacientes con CPNM mediante secuenciación de nueva generación. Implicac…
2021
A pesar del éxito de la inmunoterapia con los bloqueantes de los puntos del control inmune (ICBs) en el cáncer de pulmón, el porcentaje de pacientes que se beneficia de este tipo de tratamiento sigue siendo inferior al 30%, por lo que aún es necesario el desarrollo de nuevos posibles biomarcadores para una mejor selección de los pacientes. El objetivo principal del presente trabajo de tesis doctoral es caracterizar el repertorio de la cadena beta del receptor de células T (TCR-β) en sangre periférica y correlacionar con las variables demográficas, clínico-patológicas, predictivas y pronósticas en una cohorte de pacientes con cáncer de pulmón no microcítico (CPNM) en estadios avanzados trata…
Kriptogrāfisko valūtu ietekme uz starptautisko ekonomiku
2018
Mūsdienu pasaulē jau ir aptuveni tūkstotis dažādu kriptogrāfisko valūtu, no kurām populārākā ir bitkoins. Pieprasījums pēc šīs valūtas ir pieaudzis rekordlielā ātrumā un joprojām nav nokrities līdz tādiem skaitļiem, kādi bija pašā bitkoinu sākumā. Kriptogrāfiskās valūtas piesaista lielu uzmanību un lielākā daļa cilvēku mēģina izprast, kā tās darbojās. Pastāv daudzi viedokļi par kriptogrāfiskajām valūtām: 1. Kriptogrāfiskās valūtas noteikti mainīs pasauli. 2. Kriptogrāfiskajai valūtai piemīt burbuļa efekts un tuvākajā nākotnē tā pazudīs. 3. Kriptogrāfiskās valūtas ir jauns naudas veids, kurš sniedz dažādas priekšrocības tās lietotājiem. Gandrīz visas kriptogrāfiskās valūtas darbojās ar blokķ…
Revista electrónica de investigación y evaluación educativa
2018
La victimización en la escuela representa un fenómeno preocupante dado las consecuencias negativas que se le asocian, como son estados de depresión o ansiedad, los cuales guardan relación con los hábitos de ocio digital. Se realizó un estudio descriptivo de tipo transversal, realizado en una muestra de 1038 escolares de tercer ciclo de Educación Primaria (M = 11,33; DT = 1,27), el cual persigue como principal objetivo contrastar un modelo explicativo del uso de televisión y videojuegos en función de la victimización. Se emplearon como principales instrumentos la Escala de Victimización en la Escuela (Mynard y Joseph, 2000), el Cuestionario de Experiencias Relacionadas con Videojuegos (Chama…