Search results for "Pasture"
showing 10 items of 85 documents
Increased Goodpasture antigen-binding protein expression induces type IV collagen disorganization and deposit of immunoglobulin A in glomerular basem…
2007
Increased expression of Goodpasture antigen-binding protein (GPBP), a protein that binds and phosphorylates basement membrane collagen, has been associated with immune complex-mediated pathogenesis. However, recent reports have questioned this biological function and proposed that GPBP serves as a cytosolic ceramide transporter (CERTL). Thus, the role of GPBP in vivo remains unknown. New Zealand White (NZW) mice are considered healthy animals although they convey a genetic predisposition for immune complex-mediated glomerulonephritis. Here we show that NZW mice developed age-dependent lupus-prone autoimmune response and immune complex-mediated glomerulonephritis characterized by elevated GP…
Absence of Anti-Glomerular Basement Membrane Antibodies in 200 Patients With Systemic Lupus Erythematosus With or Without Lupus Nephritis: Results of…
2020
IntroductionAnti-glomerular basement membrane (GBM) antibodies are pathogenic antibodies first detected in renal-limited anti-GBM disease and in Goodpasture disease, the latter characterized by rapidly progressive crescentic glomerulonephritis combined with intra-alveolar hemorrhage. Studies have suggested that anti-GBM antibody positivity may be of interest in lupus nephritis (LN). Moreover, severe anti-GBM vasculitis cases in patients with systemic lupus erythematosus (SLE) have been described in the literature, but few studies have assessed the incidence of anti-GBM antibodies in SLE patients.ObjectiveThe main study objective was to determine if positive anti-GBM antibodies were present …
Aseasonal sheep and goat milk production in the Mediterranean area: Physiological and technical insights
2015
Abstract Aseasonal or out-of-season milk production for small ruminants raised in Mediterranean areas refers generally to milk having to be produced at a time that corresponds to summer which is when conditions are generally unfavourable with respect to both physiological aspects and nutritional factors related to seasonal climatic trends. In fact, sheep and goat milk production in the Mediterranean basin is mostly based on pasture utilization and thus follows the pasture availability pattern. This causes a strong seasonal pattern to the amount of milk processed by cheese processes plants, with the peak being in the spring, a marked reduction in early summer and nil or low availability of m…
Feeding and management techniques to favour summer sheep milk and cheese production in the Mediterranean environment
2015
Sheep milk production in summer can lead to a diversification of milk products and to a more regular product distribution in the market throughout the year. However, in Mediterranean countries small ruminant production cycles are subjected to seasonal variations in forage resources. During summer, a key period for out of season production systems, nutrition plays a fundamental role in regulating the quantity and quality of milk production. In this review, feeding strategies and techniques that could be applied for out of season production in dairy ewes under different Mediterranean conditions are described for intensive, pasture-based and mixed systems. The most common feeding strategies us…
Comparing different methods to determine soil physical quality in a mediterranean forest and pasture land
2016
Soil physical quality (SPQ) can be assessed by different experimental methodologies and criteria and the optimal/critical values or ranges for SPQ indicators are still approximate. Sampling soils with minimal anthropic pressures should allow improvements in SPQ assessment. Different experimental methodologies and criteria were applied to sample a Mediterranean oak forest (Quercus ilex L.) and pasture land, in Sicily, with a varying degree of anthropic disturbance. Soil water retention was determined in the laboratory and the field, using the BEST (Beerkan Estimation of Soil Transfer parameters) procedure of soil hydraulic characterization. Capacity-based indicators, the S index, and locatio…
Dot-immunobinding assay with the globular domain of collagen type IV for antiglomerular basement membrane antibodies
1988
A dot-immunobinding assay for the detection of antiglomerular basement membrane antibodies has been developed. The globular domain NC1 of basement membrane collagen type IV was used as antigen. The assay proved to be specific, sensitive, and reproducible. Circulating antibodies in each of 12 sera from patients with florid Goodpasture's syndrome could be demonstrated, whereas sera from patients with Goodpasture's syndrome in clinical remission and various control sera showed no reactivity. The advantages of the dot-blot assay are: the usage of the purified Goodpasature target antigen NCI reduces unspecific binding of IgG; only minimal amounts of antigen are required to give a positive signal…
Nation-wide study of the occurrence of Listeria monocytogenes in French soils using culture-based and molecular detection methods
2013
Identifiant HAL : hal-01120618; International audience; Soil is a potential reservoir of human pathogens and a possible source of contamination of animals, crops and water. In order to study the distribution of Listeria monocytogenes in French soils, a real-time PCR TaqMan assay targeting the phosphoribosylpyrophosphate synthetase (prs) gene of L. monocytogenes was developed for the specific detection and quantification of this bacterium within a collection of 1315 soil DNAs originated from the French Soil Quality Monitoring Network. The prs real-time PCR TaqMan assay was specific for L. monocytogenes and could quantify accurately down to 104L. monocytogenes per gram of dry soil. Among the …
Occurrence of CTX-M Producing Escherichia coli in Soils, Cattle, and Farm Environment in France (Burgundy Region).
2012
Article en open access; International audience; CTX-M [a major type of extended-spectrum beta-lactamase (ESBU] producing Escherichia coli are increasingly involved in human infections worldwide. The aim of this study was to investigate potential reservoirs for such strains: soils, cattle, and farm environment. The prevalence of bla(CTX-M) genes was determined directly from soil DNA extracts obtained from 120 sites in Burgundy (France) using real-time PCR. bla(CTX-M) targets were found in 20% of the DNA extracts tested. Samples of cattle feces (n = 271) were collected from 182 farms in Burgundy. Thirteen ESBL-producing isolates were obtained from 12 farms and further characterized for the pr…
Relationships between dietary factors and milk urea nitrogen level in goats grazing herbaceous pasture
2008
This investigation aimed to individuate the dietary factors affecting the milk urea nitrogen (MUN) concentration in goats grazing herbaceous pasture and, particularly, to verify the relationship linking the diet crude protein (CP) content to MUN. A total of 205 individual observations regarding dietary and milk variables of 37 Girgentana goats involved in two experiments were used. Goats, averaging 154±14 days in milk and 38.1±5.4 kg of live weight, grazed on swards and received 500 g/d of barley meal. Sward biomass, herbage selected by goats and individual milk yield were measured and sampled weekly. The herbage intake and diet digestibility were estimated by the n-alkane met…